Miyazaki, T. et al.. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat. Commun. 3:1236 doi: 10.1038/ncomms2231 (2012)

Futaki, Sugiko

Hayashi, Maria

Kawasaki, Miwa

Kawase, Eihachiro

Kumagai, Hideaki

Miyazaki, Takamichi

Nakatsuji, Norio

Sekiguchi, Kiyotoshi

Suemori, Hirofumi

Taniguchi, Yukimasa

Yamada, Masashi

Nature Communications

English

PubMed

細胞接着タンパクを用い、 安全・高効率なヒトES / iPS細胞の培養法を開発 : 幹細胞実用化に必要な品質保証・大量生産に向けて前進. 京都大学プレスリリース. 2012-12-05.Human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have the potential to provide an infinite source of tissues for regenerative medicine. Although defined xeno-free media have been developed, culture conditions for reliable propagation of hESCs still require considerable improvement. Here we show that recombinant E8 fragments of laminin isoforms (LM-E8s), which are the minimum fragments conferring integrin-binding activity, promote greater adhesion of hESCs and hiPSCs than do Matrigel and intact laminin isoforms. Furthermore, LM-E8s sustain long-term self-renewal of hESCs and hiPSCs in defined xeno-free media with dissociated cell passaging. We successfully maintained three hESC and two hiPSC lines on LM-E8s in three defined media for 10 passages. hESCs maintained high level expression of pluripotency markers, had a normal karyotype after 30 passages and could differentiate into all three germ layers. This culture system allows robust proliferation of hESCs and hiPSCs for therapeutic applications

Kyoto University Research Information Repository

Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells

Human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have the potential to provide an infinite source of tissues for regenerative medicine. Although defined xeno-free media have been developed, culture conditions for reliable propagation of hESCs still require considerable improvement. Here we show that recombinant E8 fragments of laminin isoforms (LM-E8s), which are the minimum fragments conferring integrin-binding activity, promote greater adhesion of hESCs and hiPSCs than do Matrigel and intact laminin isoforms. Furthermore, LM-E8s sustain long-term self-renewal of hESCs and hiPSCs in defined xeno-free media with dissociated cell passaging. We successfully maintained three hESC and two hiPSC lines on LM-E8s in three defined media for 10 passages. hESCs maintained high level expression of pluripotency markers, had a normal karyotype after 30 passages and could differentiate into all three germ layers. This culture system allows robust proliferation of hESCs and hiPSCs for therapeutic applications.Miyazaki, T. et al.. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat. Commun. 3:1236 doi: 10.1038/ncomms2231 (2012)

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http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/164626/1/ncomms2231.pdf

Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells

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