Brown, Alistair J P

Klipp, Edda

Leach, Michelle D

Tyc, Katarzyna

PLoS ONE

English

PubMed

Leach Michelle D.

Tyc Katarzyna M.

Brown Alistair J. P.

Klipp Edda

Public Library of Science (PLOS)

, a Major Fungal Pathogen of Humans

Research Archive

Modelling the regulation of thermal adaptation in Candida albicans, a major fungal pathogen of humans

Eukaryotic cells have evolved mechanisms to sense and adapt to dynamic environmental changes. Adaptation to thermal insults, in particular, is essential for their survival. The major fungal pathogen of humans, Candida albicans, is obligately associated with warm-blooded animals and hence occupies thermally buffered niches. Yet during its evolution in the host it has retained a bona fide heat shock response whilst other stress responses have diverged significantly. Furthermore the heat shock response is essential for the virulence of C. albicans. With a view to understanding the relevance of this response to infection we have explored the dynamic regulation of thermal adaptation using an integrative systems biology approach. Our mathematical model of thermal regulation, which has been validated experimentally in C. albicans, describes the dynamic autoregulation of the heat shock transcription factor Hsf1 and the essential chaperone protein Hsp90. We have used this model to show that the thermal adaptation system displays perfect adaptation, that it retains a transient molecular memory, and that Hsf1 is activated during thermal transitions that mimic fever. In addition to providing explanations for the evolutionary conservation of the heat shock response in this pathogen and the relevant of this response to infection, our model provides a platform for the analysis of thermal adaptation in other eukaryotic cells

Michelle D Leach

Katarzyna M Tyc

Alistair J P Brown

Edda Klipp

Directory of Open Access Journals

Modelling the regulation of thermal adaptation in Candida albicans, a major fungal pathogen of humans.

A 70-kilodalton recombinant heat shock protein of Candida albicans is highly immunogenic and enhances systemic murine candidiasis.

A conserved stress-activated protein kinase regulates a core stress response in the human pathogen Candida albicans.

A drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene.

A model of excitation and adaptation in bacterial chemotaxis.

A molecular mechanism for sensory adaptation based on ligand-induced receptor modification.

A synthetic heat-shock promoter element confers heat-inducibility on the herpes simplex virus thymidine kinase gene.

Abnormal proteins serve as eukaryotic stress signals and trigger the activation of heat shock genes.

Activation of the heat shock transcription factor Hsf1 is essential for the full virulence of the fungal pathogen Candida albicans. Fungal Genet Biol.

Adaptive tolerance to oxidative stress and the induction of antioxidant enzymatic activities in Candida albicans are independent of the Hog1 and Cap1-mediated pathways.

Annotation and merging of SBML models with semanticSBML.

Attributable mortality of nosocomial candidemia, revisited.

Autoantibody to heat-shock protein 90 can mediate protection against systemic candidosis.

Candida and Candidiasis.

Candida and Candidosis. 2nd ed. London, United Kingdom: Bailliere Tindall.

Cells in stress: transcriptional activation of heat shock genes.

COPASI–a COmplex PAthway SImulator.

Correlation between glutathione oxidation and trimerization of heat shock factor 1, an early step in stress induction of the Hsp response.

Correlation between synthesis of heat shock proteins and development of thermotolerance in Chinese hamster fibroblasts.

Ecology and epidemiology of Candida species.

Engineered control of cell morphology in vivo reveals distinct roles for yeast and filamentous forms of Candida albicans during infection.

Epidemiology of Candida species infections in critically ill non-immunosuppressed patients.

Evidence for a mechanism of repression of heat shock factor 1 transcriptional activity by a multichaperone complex.

Evidence for a role of Hsp70 in the regulation of the heat shock response in mammalian cells.

Fungal heat-shock proteins in human disease.

Fungal morphogenesis and host invasion.

Genetic control of heat-shock protein synthesis and its bearing on growth and thermal resistance in Escherichia coli K12.

Getting started with yeast.

Global analysis of protein expression in yeast.

Heat shock factor and the heat shock response.

Heat shock gene regulation by nascent polypeptides and denatured proteins: Hsp70 as a potential autoregulatory factor.

Heat shock proteins and thermal resistance in yeast.

Heat shock response modulators as therapeutic tools for diseases of protein conformation.

Heat shock response of Dictyostelium.

Heat shock transcription factors: structure and regulation.

Heat-inducible ATP-binding proteins of Candida albicans are recognized by sera of infected patients.

Heat-shock protein protection.

Hospitalacquired candidemia. The attributable mortality and excess length of stay.

HSP90 interacts with and regulates the activity of heat shock factor 1 in Xenopus oocytes.

Hsp90 orchestrates temperature-dependent Candida albicans morphogenesis via Ras1-PKA signaling.

Hsp90 potentiates the rapid evolution of new traits: drug resistance in diverse fungi.

Identification of sumoylation targets, combined with inactivation of SMT3, reveals the impact of sumoylation upon growth, morphology, and stress resistance in the pathogen Candida albicans.

Induced thermal tolerance and heat shock protein synthesis in Chinese hamster ovary cells.

Mathematica edition.

Mathematical modeling of the eukaryotic heat-shock response: dynamics of the hsp70 promoter.

Model validation and robust stability analysis of the bacterial heat shock response using SOSTOOLS.

Model-based inference of biochemical parameters and dynamic properties of microbial signal transduction networks.

Modulebased analysis of robustness tradeoffs in the heat shock response system.

Molecular and proteomic analyses highlight the importance of ubiquitination for the stress resistance, metabolic adaptation, morphogenetic regulation and virulence of Candida albicans.

Nonfilamentous C. albicans mutants are avirulent.

On mechanisms that control heat shock transcription factor activity in metazoan cells.

On the path to the heat shock response: destabilization and formation of partially folded protein intermediates, a consequence of protein thiol modification.

Oudenaarden A

Pathways of chaperonemediated protein folding in the cytosol.

Positive regulatory gene for temperaturecontrolled proteins in Escherichia coli.

Preclinical assessment of the efficacy of mycograb, a human recombinant antibody against fungal HSP90.

Protein degradation and protection against misfolded or damaged proteins.

Protein denaturation in intact hepatocytes and isolated cellular organelles during heat shock.

Recovery of protein synthesis after heat shock: prior heat treatment affects the ability of cells to translate mRNA.

Repression of heat shock transcription factor HSF1 activation by HSP90 (HSP90 complex) that forms a stress-sensitive complex with HSF1.

Requirement for Hsp90 and a CyP40-type cyclophilin in negative regulation of the heat shock response.

Robust perfect adaptation in bacterial chemotaxis through integral feedback control.

Robustness in simple biochemical networks.

Role of the heat shock transcription factor, Hsf1, in a major fungal pathogen that is obligately associated with warm-blooded animals.

Sensitivity analysis of stoichiometric networks: an extension of metabolic control analysis to non-steady state trajectories.

Sequential fractionation and two-dimensional gel analysis unravels the complexity of the dimorphic fungus Candida albicans cell wall proteome.

Short circuiting stress protein expression via a tyrosine kinase inhibitor, herbimycin A.

Sorribas A

Specific protection from phenocopy induction by heat shock.

Stress-induced gene expression in Candida albicans: absence of a general stress response.

Structure and regulation of the HSP90 gene from the pathogenic fungus Candida albicans.

Systems biology - a textbook.

Tetracycline-regulatable system to tightly control gene expression in the pathogenic fungus Candida albicans.

The DNA-binding activity of the human heat shock transcription factor is regulated in vivo by Hsp70. Mol Cell Biol 13: 5427–5438.

The heat shock response is self-regulated at both the transcriptional and posttranscriptional levels.

The heat-shock response in plants: physiological considerations

The heat-shock response.

The highly immunogenic enolase and Hsp70p are adventitious Candida albicans cell wall proteins.

The human heat shock protein Hsp70 interacts with Hsf1, the transcription factor that regulates heat shock gene expression.

The role of heat-shock proteins in thermotolerance

Thermotolerance and trehalose accumulation induced by heat shock in yeast cells of Candida albicans.

Transcriptional profiling on all open reading frames of Saccharomyces cerevisiae.

Varying patterns of protein synthesis in Drosophila during heat shock: implications for regulation.

Xuong NH, Geiduschek EP

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3308945

Modelling the regulation of thermal adaptation in Candida albicans, a major fungal pathogen of humans

Abstract

Similar works

Full text

Available Versions

Public Library of Science (PLOS)

Research Archive

Directory of Open Access Journals