Source and Description of Primers. Primers were designed from published sheep epidermal growth factor ( EGF) sequence (GenBank accession no. U36428) and were used to amplify across intron 3 of the EGF gene from porcine genomic DNA. Of sequences produced, 84 bp showed 82.1% identity to sheep EGF exon 3 and 81.0% to human EFG exon 3. For exon 4, the 75 bp showed 82.3% identity to sheep EGF exon 4 and 77.3% identity to human EGF exon 4. These sequences were further used to design pigspecific primers and have been submitted to GenBank (accession nos. AF079768 and AF079769)
