Sjogren's syndrome (SS), usually described as a chronic inflammation which results in xerostomia (dry mouth) and xerophthalmia (dry eyes). According to the theory of traditional Chinese medicine, body fluid impairment causes the dryness, inducing water secretion deficiency. Discovery of a family of water-specific membrane channel proteins, the aquaporins, provides an interesting molecular mechanism of water permeability and transportation which were found abnormal in tissues of SS patients. Thus, this dryness may lead to the dysfunction in organs as various systematic manifestations. We established an autoallergic mouse model in vivo, and human salivary gland cell line A-253 in vitro. Polysaccharides of Dendrobium officinale (DP) were administrated as treatment, which was described to nourish yin and promote the body fluid. Results showed that immunization with SG autoantigen induced decrease of body weight and increased water intake, decreased AQP5 expression in a series of organs related to body fluid. Sera from model mice induced apoptosis of A-253 cells with activation of caspase-3. Administration of DP could reverse these pathological changes in both the animal and cell model. Thus, DP may be a promising candidate for the treatment of SS by up-regulating the expression of AQP-5 and protecting cells from apoptosis. © 2010 The Berkeley Electronic Press. All rights reserved.published_or_final_versio

Sze, SCW

Shaw, PC

Ng, TB

Zhang, Z

Feng, Y

Chen, JP

Zhang, KY

Tong, Y

Lin, X

Sjogren's syndrome (SS), usually described as a chronic inflammation which results in xerostomia (dry mouth) and xerophthalmia (dry eyes). According to the theory of traditional Chinese medicine, body fluid impairment causes the dryness, inducing water secretion deficiency. Discovery of a family of water-specific membrane channel proteins, the aquaporins, provides an interesting molecular mechanism of water permeability and transportation which were found abnormal in tissues of SS patients. Thus, this dryness may lead to the dysfunction in organs as various systematic manifestations. We established an autoallergic mouse model in vivo, and human salivary gland cell line A-253 in vitro. Polysaccharides of Dendrobium officinale (DP) were administrated as treatment, which was described to nourish yin and promote the body fluid. Results showed that immunization with SG autoantigen induced decrease of body weight and increased water intake, decreased AQP5 expression in a series of organs related to body fluid. Sera from model mice induced apoptosis of A-253 cells with activation of caspase-3. Administration of DP could reverse these pathological changes in both the animal and cell model. Thus, DP may be a promising candidate for the treatment of SS by up-regulating the expression of AQP-5 and protecting cells from apoptosis.published_or_final_versio

HKU Scholars Hub

Journal of Complementary and Integrative Medicine

Protective effect of Dendrobium officinale polysaccharides on experimental Sjogren's syndrome

Besides providing comparable resolution as light microscope, acoustic microscope (AM) has its unique application in the area material characterization. Material characterization using AM is based on the measurement of leaky surface wave speed, which is extracted from the so-called V(z) curve[1]. In this V(z) technique, usually a tonebust mode of operation is required and relatively higher equipment cost is needed.</p

Yang, Che-Hua

Digital Repository @ Iowa State University (ISU)

CHARACTERIZATION OF PIEZOELECTRICS USING LINE-FOCUS TRANSDUCER Che-Hua Yang Department of Mechanical Engineering Chang Gung University 259 Wen-Hua 1 st Rd. Kwei-Shan, Taoyuan, Taiwan INTRODUCTION Besides providing comparable resolution as light microscope, acoustic microscope (AM) has its unique application in the area material characterization. Material characterization using AM is based on the measurement of leaky surface wave speed, which is extracted from the so-called Vez) curve[l]. In this V(z) technique, usually a tone-bust mode of operation is required and relatively higher equipment cost is needed. In 1980 Kushibiki et. a!. [2] developed a line-focus-bcam(LFB) transducer for the acoustic microscope system. The LFB transducer is polarization-sensitive and enables acoustic microscopists to measure surface wave speeds of anisotropic materials, such as composites, along different polarization directions. Recently, Xing et. a!. [3] developed a lens-less LFB transducer that is compatible with a pulscrlreceiver system common in nondestructive applications, but trading off the high spatial resolution which is not always necessary in all material characterizations. With larger dimension ofthe LFB transducer, a short pulse time-resolved measurement on the leaky surface wave speed is possible. In the current research, the lens-less LFB transducer is used to characterize piezoelectric material. First of all, the time-resolved technique is extended to measure the wave speeds of leaky Lamb modes for thin isotropic specimens. At the same time, a double fast Fourier transform(FFT) signal processing procedure is developed to convert the time-domain data to V(z) information. Finally, these two techniques are used simultaneously to characterize piezoelcctic lithium niobate (LiNb01) plate. EXPERIMENTAL SETUP The experimental setup is very common in most standard C-scan NDE systems. As shown in Figure I, the system consists of a line-focus transducer, an X-Y-Z scanner, a motion controller for the scanner, a pulserlreceiver, a digital oscilloscope, and a personal Review of Progress in Quantitative Nondestructive Evaluation. Vol 17 Edited by D.O. Thompson and D.E. Chimenti, Plenwn Press, New York, 1998 177 computer. The line-focus transducer with a PVDF film as active element is manufactured in NISI. Focal length of the transducer is 25.4 mm. The transducer has a center frequency of 10 MHz. A Panametrics 5800PR pulser/receiver is used to generate pulse to drive the line-focus transducer, and then serve as receiver to detect the signal returning from the transducer. The pulser/receiver and the transducer work in a pulse/echo mode. The detected signal is then recorded with a LeCroy 931 OA digital oscilloscope, and then transferred to a personal computer through GPIB bus for further data processing. Motion of the transducer is based on a mechanical scanner controlled by the computer through the motion controller. MEASUREMENTS After the transducer is precisely aligned, the transducer is first moved vertically, or in the z direction as shown in Figure 1, relative to the sample surface such that the focal line of the transducer lies on the upper sample surface. At the same time, the reflected signal is recorded and stored in a time series of voltage signal. In the acquisition of the signal, the digital oscilloscope is triggered at the direct reflection signal from the upper surface of the sample. After the focal line is well adjusted, the transducer is then scanned towards the sample to obtain a set of data corresponding to various levels of defocus. The whole set of data is then further analyzed. The first sample is an aluminum plate with a thickness of20.0 mm. Figure 2 is the recorded signal recorded signal corresponding to various levels of defocus of the line-focus transducer on a 20.0 mm thick aluminum plate. To represent the recorded signal as a flmction of defocus more clearly, we introduce an image representation format similar to the traditional B-scan as show in Figure 2. In Figure 2, the horizontal axis is the time delay from the directly reflected signal; while the vertical axis is the defocus. In this image representation, we see very clearly the directly reflected signal from the upper surface forming the vertical line sitting in the left-hand side of the image, and the leaky Rayleigh wave with positive slope. As [1] discovered, the slope ofthis positive line can be used to determine the wave speed of leaky Rayleigh wave with very high accuracy. We will discuss this so-called time-resolved technique in the next section. y ~~x Motion k ~\ - Contl"ollcr r--z 5800 PR -Pul erlReccivcr -I Per onal ~ J - omputcr 9310 I Oi ital OscillosCODC = I Figure 1. A schematic showing the experimental setup 178 It is interesting when the thickness of the plate sample is reduced. To see the line-focus transducer interacting with a thin specimen, we test another aluminum plate sample of a thickness of 1.48 mm. The recorded signal as a function of defocus representing in a image format is seen in Figure 2(b). As shown, this image is more complicated than that in Figure 2(a), which corresponds to a thick aluminum specimen of20.0 mm thick. There are more line features in this image. First of all, we notice that in Figure 2(b) there are extra vertical line which are absent in the thick specimen. These vertical lines are the back echoes from the lower surface of the thin plate. They go beyond the horizontal range of in the case of thick specimen. Same as the thick specimen, we see very clearly the lealy Rayleigh wave. Besides the Rayleigh wave, there are extra modes showing in the image, which is currently under investigation. THE TIME-RESOLVED TECHNIQUE Figure 2 is ready for the determination of the wave speed of leaky guided waves, or leaky Rayleigh wave for the highfd regime. As [1] suggested, the wave speed of the leaky guided wave can be determined by the following equation: (1) where Vw is the wave speed in the coupling media(water), and dz / dt is the slope of the line feature corresponding to the leaky guided wave in Figure 2. To calculate the wave speed, first of all, we find the peak positions of the line feature corresponding to the leaky guided mode. Then the slope can be determined by a linear regression of the peak positions. For the case of thick aluminum specimen as in Figure 2(a), we can easily determine the wave speed of the leaky Ralyeigh wave by equation (I) to be 2975 m/sec. For the case of thinner specimen as 1.48 mm thick aluminum plate, however, we are detecting the group Z=8mm t= 2 us Alummum(2ot) z=10mm t= 2 us Alum (1.48t) Figure 2. (a) an image representation format showing the recorded signal in subsequent defocus for an aluminum plate of20.0 mm thick, (b) aluminum plate of 1.48 mm thick. 179 velocity of leaky Lamb modes traveling at the group velocity of the frequency near the center frequency of the transducer to the particular defocus. In the case of the current experiment, the dominant leaky Lamb mode is the zero-th order anti-symmetric mode, or the AI) mode. In the measurement of the leaky Lamb wave speed based on the time-resolved technique, one needs to be a little bit more careful. The risk comes from the coupling of the dispersion characteristics of Lamb wave and non-uniform response of the line-focus transducer in the course of defocusing. First of all, group velocity for the All mode leaky Lamb wave is dispersive, particularly in the low(d regime. On the other hand, as pointed by Kushibiki in [5], the response of an acoustic microscope system tends to bias to lower frequency as defocus increasing. For the time-resolve technique, essentially we measure the slope the line feature corresponding to the leaky Lamb wave as the defocus increases. THE V(z) TECHNIQUE BASED ON PULSE DATA In acoustic microscopy, V(z) curve is very useful for material characterization. Very often in acoustic microscopy, tone-burst signals are used to drive the transducer to obtain V(z) curve. The equipment to generate and detect tone-bust signals are much more complicated than a pulserlreceiver system which are being used in the current research. Here, we propose a method to obtain V(z) curves for the use of material characterization by using handy NDE equipment such as pulserlreceiver. In this method, short pulse signals are generated and detected using the pulserlreceiver. Then, the detected pulsed signals are Fourier transformed with respected to time for all levels of defocus. After then, frequency spectra versus defocus can be obtained. By the selection of a working frequency within the Figure 3. Relative signal amplitude as functions of both frequency (f) and defocus(z) after Fourier transforms with respect to time. Horizontal axis is frequency ranging from 0 to 20 MHz. Vertical axis is proportional to defocus, 0 at the bottom and 8 mm at the top. 180 sensitivity of transducer, the magnitude is plotted against defocus, z. V(z) curve is just the magnitude-defocus relation in the plot. Take the aluminum sample of20.0 mm thickness for example, previously we have shown the time-domain data in Figure 2(a). After Fourier transforms with respect to time, we obtain the relative signal amplitude as a function of both frequency (f) and defocus(z) as shown in Figure 3. Simply by selecting an arbitrary working frequency, say 7 MHz for example, we plot the relative magnitude of the frequency spectra at 7 MHz versus the defocus based on the results in Figure 3 to obtain the V(z) curve. The result is shown in Figure 4(a). As one can recognize very easily, Figure 4(a) is a typical V(z) curve, which is familiar to all the society of acoustic microscopy. The spatial period of z, known as L1Z , in the V(z) curve can be used to calculate the wave speed of leaky Rayleigh wave, or even leaky Lamb modes, through the following equation: 30 05 00 Defocus (om) < (llmm) Figure 4. (a) V(z) curve corresponding to a working frequency of7 MHz for the 20 mm thick aluminum plate. (b) V(k) curve after Fourier transform w.r.t. z from Figure 2(a). A LUMI N UM , t- 20 .0 mm • 0 4 0 0- 38 I Time-Resolved Technique I 38 OJ en V(z) Technique E 36 36 ~ -' 3. 3 • W > 32 3 2 W ~ 30 3.0 . " . I 2 8 28 G W 26 26 -' >-« 2' 2 • a:: 22 2.2 20 2 0 2 10 12 Frequency (M Hz) Figure 5. The measurements of leaky Rayleigh wave velocity as a function of frequency based on the time-resolved technique and the V(z) technique. 181 2f (1 - cos e(! ) (2) Here, /',.z is the period in the V(z) curve. w and ,10 are the wave speed and wavelength in the fluid. e(! is the critical angle contains the information about the wave velocity of the guided wave, (! , through the Snell's Law: . e Vw Slll (! = - (3) , Va Equation (2), together with equation (3) are used to calculate the wave speed of leaky guided waves from the V(z) curve. Sometimes the determination of the defocus period, /',.z , is difficult, especially in the presence of dispersion nature of the guided wave. To attack this difficulty, a spatial Fourier transform with respect to z is taken to obtain the V(k) curve. Again taking 20 mm thick aluminum plate for example, the V(k) curve in Figure 5(b) is after a Fourier transform with respect to z from Figure 5(a). For the thick specimen measured with the line-focus transducer around 10 MHz, we are working in a highfd regime, in which the Olh order Lamb modes are already converged to Rayleigh wave. It is interesting to check this assumption from the measured leaky guided wave speed as a function of the selected working frequency. The results are plotted in Figure 6. Shown in Figure 6 are the measurements ofleaky guided wave velocity as a function of working frequency based on time-resolved technique and V(z) technique. The non-dispersive assumption on Rayleigh wave is verified by the measurement based on the V(z) technique. Sine the time-resolved technique contains no dispersion information, the Rayleigh wave speed in Figure 5 ought to be a horizontal line. CHARACTERIZATION OF PIEZOELECTRIC PLATE An X-cut lithium niobate (LiNb03) piezoelectic plate of 1.0 mm thickness is characterized using the line-focus transducer. Figure 6 shows the relation between the crystal axis and the curvature plane of the line-focus transducer. As shown in Figure 6, the Z-axis of the line-focus transducer is parallel to the -X crystal direction. The azimuthal angle, ~, is used to described the angle between the crystal Z direction and the transducer's curvature plane. Since the piezoelectric plate interacts with the surrounding fluid not only through mechanical boundary conditions, but also trough electrical boundary conditions, it is very important to control the electrical property of the coupling fluid. In the current experiment, we use de-ionized pure water with conductivity less than 8 f..lS/cm. Following the same experimental procedure described above, reflected signal as a function of defocus is represented in an image format as shown in Figure 7. In the test, we make a scan for an increment in ~ of 5 degrees, starting form 0 to 180 degrees. Figure 7(a) is a scan for ¢ = 0° , and 8(b) for ~ of 105 degree. For the measurements in LiNb03 plate, the total defocus is 6 mm, and the time period of recording is 2 f..ls. Again, from Figure 7, sitting in the left side of the image. The line with positive slope corresponds to the leaky Lamb mode. It is readily observed that the slope for ¢ = 105() is apparently higher than that ¢ = 0° , which implies higher wave velocity for the Olh order Lamb mode for ¢ = 10511 • 182 z 1 c====J Transducer X Z ~ eakyG uidedWave E Propagation Direction LiNb03 ~~~==~ ~ y Figure 6. A schematic showing the crystal direction and the propagation direction of the leaky guided wave in test with the line-focus transducer. z-6mm t- 2 US >< Lit 03 Phl- 0 Figure 7. Record the reflected signal with progressive defocusing for Imm thick LiNbO] plate represented in image format (a) ¢ = 0° ,(b) ¢ = 1050 0-Q) Vl 3700 E 3600 z: '0 o Q; 3500 > Q) > ~ 3400 Q) o ~ 3300 ::l (/) y 00 o '0 0 0 <> 0 0 0 0 () o 0 X-cut LiNb03 (1mm) z a - - Campbell's Short·Clrcult Model 0- Time-Resolved Techrllque o V(:) Technique o 3200 ~~~~~~~~~~~~~~~~~~~~~~~~ o 20 W ~ ~ 100 lW lW 160 1 ~ Direction of Propagation (q, ) Figure 8. Leaky surface wave speed of an X-cut LiNbO] piezoelectic plate measured by time-resolved and V(z) techniques. 183 To calculate the wave velocity of leaky Lamb wave, we use both the time-resolved technique and V(z) technique as described earlier. The results are shown in Figure 8, in which the measured leaky surface wave velocity using the time-resolved technique and V(z) technique is plotted as function of $. At the same time, theory developed by Campbell et. al. [7] for the predictions of surface wave velocity for the short-circuit boundary condition is plotted for comparison. As seen in Figure 8, measurements of leaky surface wave based on both time-resolved technique and V(z) techniques demonstrate the anisotropic nature of the X-cut LiNb03 piezoelectic plate following the same trend as Cmapbell's theory predicted. However, the time-resolved technique has more fluctuations, and the V(z) technique constantly measuring lower surface wave velocity. CONCLUSIONS In this research, a line-focus PVDF transducer driven by a simple pulser/receiver system is used to measure the wave speeds of leaky surface wave in a thick isotropic plate and leaky surface wave in a thin plate. An image format is adopted for the representation of the reflected signal in progressive defocus scanning. With this image representation, we observed complicated features in the time-domain in thin specimen. Furthermore, we develop a method to measure the V(z) curve using the pulse/echo signal from the line-focus transducer without using tone-bust systems. Finally, a X -cut LiNb03 piezoelectic plate is tested. Measurements on leaky surface wave based on both time-resolved and V(z) techniques demonstrate the same anisotropic nature of the X-cut LiNb03 piezoelectic plate same as theoretical predictions. ACKNOWLEDGMENTS Thanks for Dr. Xiang and Dr. Blessing at NIST for supplying the transducer. This research is partially supported by NSC Taiwan through the grant No. NSC86-2314-B-I82-I05-M08. REFERENCES 1. R. D. W glein and R. G. Wilson, "Characteristic material signatures by acooustic microscopy, " Electron Lett., Vo1.14, 1978. 2. D. Xiang, N.N. Hsu, and G.Y. Blessing, "The design, construction and applications ofa large aperture lens-less line-focus PVDF transducer, "Ultrasonics 34, 1996. 3. D. Xiang, N.N. Hsu, and G.Y. Blessing, "Materials characterization by a time-resolved and polarization-sensitive ultrasonic technique, " Review of Progress in Quantitative Nondestrutive Evaluation, vol. 15, 1997. 4. AD. Briggs, Acoustic Microscopy, Clarrendon Press, Oxford,I992. 5. B.A. Auld, Acoustic Fields and Waves in Solids, Rober E. Krieger Publishing Company, Florida, 1990. 6. 1. Kushibiki and N. Chubachi, "Material characterization by line-focus-beam acoustic microscope, " IEEE Transactions on Sonics and Ultrasonics, Vol. SU-32, 1985. 7. P. Mutti, A. Briggs, and D. Bowler, "Oscillations in V9z) curves of thin samples, "IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control, Vol. 42, No. 4,1985. 184 

Characterization of Piezoelectrics Using Line-Focus Transducer

Che-Hua Yang

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http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=3614&context=qnde

Protective effect of Dendrobium officinale polysaccharides on experimental Sjogren's syndrome

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