May 10, 1947, a I-year old Duroc gilt was brought to the Stange Memorial Clinic with an abscess of the right scapular region, with a fistulous tract discharging pus over the right shoulder area. The temperature of the gilt was elevated to 104.8° F

Kilpatrick, W. J.

English

May 10, 1947, a I-year old Duroc gilt was brought to the Stange Memorial Clinic with an abscess of the right scapular region, with a fistulous tract discharging pus over the right shoulder area. The temperature of the gilt was elevated to 104.8° F.</p

Kilpatrick, W.

Digital Repository @ Iowa State University (ISU)

Corynebacterium Psuedotuberculosis in a Gilt W. J. Kilpatrick, V. M. 3 May 10, 1947, a I-year old Duroc gilt was brought to the Stange Memorial Clinic with an abscess of the right scapu-lar region, with a fistulous tract discharg-ing pus over the right shoulder area. The temperature of the gilt was elevated to 104.8° F. May 12, 1947, the right shoulder area was cleaned and shaved, the skin in the area was infiltrated with 2 percent procaine hydrochloride. The fistulous tract was probed and an opening was made at the lower extent of the abscess to es-tablish drainage. A seton was put in place and the wound was irrigated with liquid Bipp. Following the original treatment, the wound was irrigated daily with potasium permanganatel: 3000, and Bipp was injected into the abscess. May 31, 1947, the gilt was brought to the post mortem laboratory and de-stroyed. Post mortem examination re-vealed caseous lymphadenitis, a healing abscess in the scapular region, purulent pneumonia, pericardial adhesions, and chronic purulent perivulvitis. Most of the peripheral lymph nodes showed chronic purulent lymphadenitis. Ascarids and lungworms were also found. May 12, 1947, following the external cleaning of the right shoulder area, a ster-ile swab was inserted into the abscess to obtain samples of the exudate for bacter-iological cultures. By streaking the exu-date on blood agar plates, the organism Corynebacterium pseudotuberculosis was isolated and identified. A gram negative rod, resembling Pasteurella hemolytica, was also isolated, but was regarded as be-ing of secondary importance. During the post mortem examination, smears from the prefemoral lymph nodes were made on blood agar plates from these. Corynebacterium psuedotuberculo-sis was again isolated and identified. Direct Summe1', 1948 tissue smeaci from the prefcmoral lymph nodes, made on slides during the post mortem examination, showed gram posi-tive rods with distinct metachromatic granules. Colonies of Corynebacterium psuedotu-berculosis on the blood agar plates were dry, crumbly, and orange colored. The organism isolated from the exudate of the abscess on May 12, produced acid without gas from dextrose and maltose, did not ferment sucrose, salicin, mannite, raffinose, inulin, trehalose, or sorbitol. IF1dol was not produced and nitrates were not re-duced. Growth extended up the sides of the tubes from the surface of fluid media; some of the surface growth settled to the bottom of the tube, forming a coarse, granular sediment. When this organism was examined again two weeks later, it did not ferment dextrose or maltose dur-ing an observation period of two weeks. The organism isolated from the prefemoral lymph nodes during the post mortem ex-amination did not ferment dextrose or maltose, and was in every other respect identical with the organism isolated from the abscess. Although the organism did not ferment carbohydrates in the manner attributed to it in classical descriptions, it was re-garded as Corynebacterium psuedotuber-culosis because of its staining properties, orange colored dry colonies, tube growth, sedimentation, and other growth charac-teristics. The presence of Corynebacterium psue-dotuberculosis in swine is rare. No pre-vious cases are believed to have been re-ported. The post mortem picture was similar to that presented by caseous lym-phadenitis in sheep, a disease also caused by Corynebacterium psuedotuberculosis, and characterized by caseation necrosis in the lymph nodes. Corynebacterium psue-dotuberculosis also is the etiological agent of ulcerative lymphangitis of horses. In deer, the organism causes subcutaneous abscesses on the extremities. References: 1. KeIser, Raymond A.: Manual of Veterinary Bacteriolo!Zy, 1938. 2. Merchant, I. A.: Veterinary Bacteriology, 1946. 141 

Corynebacterium Psuedotuberculosis in a Gilt

https://dr.lib.iastate.edu/handle/20.500.12876/45522

Corynebacterium Psuedotuberculosis in a Gilt

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