Azam, F

Jain, S

Unni, H N

Research Archive of Indian Institute of Technology Hyderabad

Advanced Microfluidic Mixing Device for the Study of Macromolecule DynamicsShubha Jain, F. Azam, Dr. H. N. UnniDepartment of Biomedical, IIT HyderabadIntroductionMicromixing is a crucial process within microfluidic systems such as micro total analysis systems. We havedemonstrated a microfluidic mixer device to characterized and study the macromolecule dynamics such as kinetics ofprotein folding, DNA/RNA sequencing, single molecule study and detection etc. Geometric variation such as length,height, geometric shape and number of channel helps to enhance the mixing efficiency without applying any externalforce or energy. Low Reynolds number is considered to control the viscous force, thus the flow would be laminar. TheComsol Multiphysics platform has been used to study the simulation.In particular we use Laminar flow (spf) and Transported diluted species (tds) for the simulation of microchannel fluidmixing by coupling of convective-diffusion and Navier-Stokes equation. We chose two fluid with different concentrationof 0 and 1 mol/m3 respectively. Ideally the last reservoir will give the average concentration of both fluids i.e. 0.5 mol/m3.MethodGeometry Velocity ConcentrationResult and DiscussionAs a result we achieved the complete mixing at the end reservoir. The significant mixing has been performed with±0.03 mol/m3 tolerances. The efficiency of mixing is 96 with the corresponding values of 0.47 and 0.53 mol/m3.As we achieved the complete mixing in microchannel. Using the information gathered from the above simulation, byintegrating the concept of microfluidic mixer with magnetic beads, make feasible to study the next-generationsequencing applications like genomic DNA sequencing of microbial species from single cell and protein RNAinteraction.ConclusionReferences1. Brian M Paegel et.al, Current Opinion in Biotech (2003),14: 42-50.2. T.N.T. Nguyen et.al, Sensors and Actuators B (2008), 132:172–181.3. Elmabruk A. Mansur et.al, Chem Eng Journal (2008),16: 4.4. Lorenzo Capretto et.al, Top Curr Chem (2011), 304: 27–68.5. Shakhawat Hossain et.al, Chem Eng Journal (2009), 150: 492–501.Advances in Next Generation SequencingConvection and Diffusion𝜌𝜕𝑢𝜕𝑡+ 𝑢 ∙ 𝛻𝑢 = −𝛻𝑝 + 𝛻 ∙ (𝜇 𝛻𝑢 + 𝛻𝑢 𝑇 − 23𝜇 𝛻.𝑢 𝐈) + 𝐅Navier-Stokes Equations𝛻 ⋅ u = 0

Advanced Microfluidic Mixing Device for the Study of Macromolecule Dynamics

Name not available

Advanced Microfluidic Mixing Device for the 
Study of Macromolecule Dynamics
Shubha Jain, F. Azam, Dr. H. N. Unni
Department of Biomedical, IIT Hyderabad
Introduction
Micromixing is a crucial process within microfluidic systems such as micro total analysis systems. We have
demonstrated a microfluidic mixer device to characterized and study the macromolecule dynamics such as kinetics of
protein folding, DNA/RNA sequencing, single molecule study and detection etc. Geometric variation such as length,
height, geometric shape and number of channel helps to enhance the mixing efficiency without applying any external
force or energy. Low Reynolds number is considered to control the viscous force, thus the flow would be laminar. The
Comsol Multiphysics platform has been used to study the simulation.
In particular we use Laminar flow (spf) and Transported diluted species (tds) for the simulation of microchannel fluid
mixing by coupling of convective-diffusion and Navier-Stokes equation. We chose two fluid with different concentration
of 0 and 1 mol/m3 respectively. Ideally the last reservoir will give the average concentration of both fluids i.e. 0.5 mol/m3.
Method
Geometry 
Velocity Concentration
Result and Discussion
As a result we achieved the complete mixing at the end reservoir. The significant mixing has been performed with
±0.03 mol/m3 tolerances. The efficiency of mixing is 96 with the corresponding values of 0.47 and 0.53 mol/m3.
As we achieved the complete mixing in microchannel. Using the information gathered from the above simulation, by
integrating the concept of microfluidic mixer with magnetic beads, make feasible to study the next-generation
sequencing applications like genomic DNA sequencing of microbial species from single cell and protein RNA
interaction.
Conclusion
References
1. Brian M Paegel et.al, Current Opinion in Biotech (2003),14: 42-50.
2. T.N.T. Nguyen et.al, Sensors and Actuators B (2008), 132:172–181.
3. Elmabruk A. Mansur et.al, Chem Eng Journal (2008),16: 4.
4. Lorenzo Capretto et.al, Top Curr Chem (2011), 304: 27–68.
5. Shakhawat Hossain et.al, Chem Eng Journal (2009), 150: 492–501.
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Convection and Diffusion
𝜌
𝜕𝑢
𝜕𝑡
+ 𝑢 ∙ 𝛻𝑢 = −𝛻𝑝 + 𝛻 ∙ (𝜇 𝛻𝑢 + 𝛻𝑢 𝑇 − 23𝜇 𝛻.𝑢 𝐈) + 𝐅
Navier-Stokes Equations
𝛻 ⋅ u = 0


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Advanced Microfluidic Mixing Device for the Study of Macromolecule Dynamics

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