A Simple and Rapid Method for Determination of Domoic Acid in Shellfish

Abstract

In this paper a rapid and simple high performance liquid chromatographic method with UV absorption detection at 242 nm for determining domoic acid in shellfish samples is reported. Domoic acid was extracted from shellfish tissue by using a mixture of methanol-water (50:50, v/v). The analyses were performed on a Water symmetry C-8 (4.6 x 250 mm, 5 num) achieving an excellent separation, more than 3 min, between the analyte and tryptophan (a well known interference) in a short time of analysis (15 min lasts the complete run). The in-house validation of the method provided good results with respect to repeatability (Relative Standard Deviation (RSD) minor or equal to 2.21 percent) and recovery (minor or equal to 91.2 per cent). Domoic acid was not found when the method was applied to shellfish products purchased on local markets.JRC.I.5-Physical and chemical exposure