Demidchik, V.

Sokolik, A.

Yurin, V. M.

English

Электронная библиотека БГУ

Ответ растительной клетки на абиотические стрессовые воздействия
85
PLANT PROGRAMMED CELL DEATH IS RELATED TO ROS-ACTIVATED
K+ EFFLUX, WHICH IS MEDIATED BY K+ OUTWARDLY RECTIFYING
CHANNEL GORK
Demidchik V. 1, Sokolik A.1, Yurin V.M.1
Department of Physiology and Biochemistry of Plants, Belarusian State University,
220030, Independence Avenue 4, Minsk, Belarus; dzemidchyk@bsu.by
In animal cells, KCNH2 channel is directly activated by reactive oxygen species (ROS)
and responsible for induction of programmed cell death (PCD) through the loss of K+ and
activation of K+-regulated endonucleases/cell death proteases. Here, it was demonstrated
that NaCl and pathogen induced PCD symptoms, such as loss of electric membrane poten-
tial, electrolyte leakage and membrane integrity can be delayed in gork1-1 (lacking major
K+ efflux channel) and after application of specific K+ channel and ROS scavengers an-
tagonists (wild type Arabidopsis thaliana WS plants). K+ channel blockers and HR scav-
engers prevented stress-induced PCD in the wild type. Using CaspACE™ FITC-VAD-
FMK In Situ Marker (Promega), it was found that cell death caspase-like protease activi-
ties (triggered by salt stress, elicitors and hydroxyl radicals) increase in K+-dependent
manner in Arabidopsis thaliana root cell cytoplasm. Moreover, DeadEnd™ Fluorometric
TUNEL tests showed that stress/ROS-inducible endonuclease activity is also regulated by
cytosolic K+. Gork1-1 plants demonstrated significant delay in activation of both caspase-
like activities and endonucleases. Overall, results of this study strongly suggest that plants
and animals share PCD cascade involving Shaker group of redox-sensitive K+ outwardly
rectifying channels.


Plant programmed cell death is related to ROS-activated K+ efflux, which is mediated by K+ outwardly rectifying channel GORK

http://elib.bsu.by/bitstream/123456789/33847/1/049.pdf

Plant programmed cell death is related to ROS-activated K+ efflux, which is mediated by K+ outwardly rectifying channel GORK

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