Conference Theme: Translating Evidence into PracticeLate-Breaking Poster Session 3: abstract no. EFIC5-1288  / Poster board no. 508published_or_final_versio

Cheung, CW

Pan, Z

Qiu, Q

Sun, LT

Tai, WL

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TitleInhibition Of Spinal Endothelin Type A Receptor AmelioratesNeuropathic Pain Through Down-regulation Of Mapk And Nf--κappab P65 In RatsAuthor(s) Tai, WL; Sun, LT; Pan, Z; Qiu, Q; Cheung, CWCitation9th Congress of the European Pain Federation EFIC: Pain inEurope IX: Translating Evidence into Practice, Vienna, Austria, 2-5 September 2015Issued Date 2015URL http://hdl.handle.net/10722/216573Rights Creative Commons: Attribution 3.0 Hong Kong License  EFIC5-1288 LATE-BREAKING POSTER SESSION III  INHIBITION OF SPINAL ENDOTHELIN TYPE A RECEPTOR AMELIORATES NEUROPATHIC PAIN THROUGH DOWN-REGULATION OF MAPK AND NF-ΚAPPAB P65 IN RATS L. Tai1, L. Sun1, Z. Pan2, X. Luo1, Q. Qiu1, C. Cheung1 1ANAESTHESIOLOGY, THE UNIVERSITY OF HONG KONG, HONG KONG, Hong Kong China 2JIANGSU PROVINCE KEY LABORATORY OF ANESTHESIOLOGY, XUZHOU MEDICAL COLLEGE, XUZHOU, China Background and aims Endothelin type A receptor (ETAR) plays a critical role in Endothelin-1 (ET-1)-mediated neuropathic pain, a disease in which the mitogen activated protein kinases (MAPK) family and nuclear factor NF-κB p65 signaling take important parts. Results of our past study showed that inhibition of spinal ETAR alleviates neuropathic pain, but the underlying mechanism remains unknown. Therefore, we hypothesized that inhibition of central ETAR ameliorates neuropathic pain through modulation of the MAPK family and NF-κB p65. Methods Rats were subjected to sciatic nerve ligation (SNL) or sham operation with or without ETAR antagonist, BQ-123, administered intrathecally via implanted catheter at dosages 30μg, 60μg, or 90μg daily for 3 days respectively. Mechanical allodynia was assessed daily 30 minutes before-/after-injection, 1 hour after-injection of BQ-123 from post-SNL day 4 to 6, and once on day 7 (without BQ-123 administration) before rats were sacrificed.  Results  SNL sensitized animal’s pain response to von frey filaments applied to the ipsilateral hind paw. This observed mechanical allodynia was reduced by inhibition of spinal ETAR at all dosages significantly (P Conclusions Spinal ETAR inhibition alleviated neuropathic pain in part through inactivation of MAPK family and NF-κB p65.   

Inhibition Of Spinal Endothelin Type A Receptor Ameliorates Neuropathic Pain Through Down-regulation Of Mapk And Nf--κappab P65 In Rats

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Inhibition Of Spinal Endothelin Type A Receptor Ameliorates Neuropathic Pain Through Down-regulation Of Mapk And Nf--κappab P65 In Rats

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