Conference Theme: Tomorrow’s medicines: pharmacology, patients and populationsINTRODUCTION: Perivascular adipose tissue (PVAT) exhibits brown adipose features and its dysfunction is implicated in cardiovascular diseases. SIRT1 is key to adipocyte phenotypes and metabolism. AIMS: In the present study, we aim to investigate the role of SIRT1 within PVAT in modulating obesity-evoked endothelial dysfunction. Methods. Wild type (WT) and adipocyte-specific SIRT1 knockout mice (AKO) were fed with standard chow or westernized diet for 12 weeks. Endothelium-dependent relaxation (EDR) in aortic rings with or without PVAT was assessed by wire myograph. DHE staining was …published_or_final_versio
