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research
Asymmetric-detection time-stretch optical microscopy (ATOM) for high-contrast and high-speed microfluidic cellular imaging
Authors
KY Ho
KS Lau
+5 more
HC Shum
YH Tang
KKM Tsia
KKY Wong
TW Wong
Publication date
1 January 2014
Publisher
'SPIE-Intl Soc Optical Eng'
Doi
Cite
Abstract
High-throughput cellular imaging is acclaimed as captivating yet challenging in biomedical diagnostics. We have demonstrated a new imaging modality, asymmetric-detection time-stretch optical microscopy (ATOM), by incorporating a simple detection scheme which is a further advancement in time-stretch microscopy - a viable solution to achieve high-speed and high-throughput cellular imaging. Through the asymmetric-detection scheme in ATOM, the time-stretch image contrast is enhanced through accessing to the phase-gradient information. With the operation in the 1 μm wavelength range, we demonstrate high-resolution and high-contrast cellular imaging in ultrafast microfluidic flow (up to 10 m/s) by ATOM - achieving an imaging throughput equivalent to 100,000 cells/sec. © 2014 SPIE.published_or_final_versio
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Last time updated on 01/06/2016