Duan, Wei-na

Jiang, Ying

Lei, Shao-qing

Liu, Hui-min

Meng, Qing-tao

Su, Wa-ting

Sun, Qian

Wu, Yang

Xia, Zheng-yuan

Xia, Zhong-yuan

PLoS ONE

PubMed

Ginsenoside Rb1 (RB1), the most clinically effective constituent of ginseng, possesses a variety of biological activities. The objectives of this study were to investigate the protective effects of RB1 and its underlying mechanism on renal injury induced by intestinal ischemia-reperfusion (IIR) in mice. RB1 was administered prior to inducing IIR achieved by occluding the superior mesenteric artery for 45 min followed by 120 min of reperfusion. All-trans-retinoic acid (ATRA) was used as an inhibitor of NF-E2-related factor-2 (Nrf2) signaling. Adult male C57BL/6J mice were randomly divided into six groups: (1) sham group, (2) IIR group, (3) RB1 group, (4) sham + ATRA group, (5) IIR + ATRA group, and (6) RB1 + ATRA group. Intestinal histology and pathological injury score were observed. Intestinal mucosal injury was also evaluated by measuring serum diamine oxidase (DAO). Renal injury induced by IIR was characterized by increased levels of histological severity score, blood urea nitrogen (BUN), serum creatinine (Scr) and neutrophil gelatinase-associated lipocalin (NGAL), which was accompanied with elevated renal TUNEL-positive cells and the Bcl-2/Bax expression ratio. RB1 significantly reduced renal injury and apoptosis as compared with IIR group, which was reversed by ATRA treatment. Immunohistochemistry and Western blot analysis demonstrated that RB1 significantly upregulated the protein expression of heme oxygenase-1 (HO-1) and Nrf2, which were attenuated by ATRA treatment. Taken together, these results suggest that the protective effects of RB1 pretreatment against renal injury induced by IIR are associated with activation of the Nrf2/ anti-oxidant response element (ARE) pathway

Qian Sun

Qing-tao Meng

Ying Jiang

Hui-min Liu

Shao-qing Lei

Wa-ting Su

Wei-na Duan

Yang Wu

Zheng-yuan Xia

Zhong-yuan Xia

Directory of Open Access Journals

Protective effect of ginsenoside Rb1 against intestinal ischemia-reperfusion induced acute renal injury in mice.

Crossref

Protective Effect of Ginsenoside Rb1 against Intestinal Ischemia-Reperfusion Induced Acute Renal Injury in Mice

Sun, Q

Meng, QT

Jiang, Y

Liu, H

Lei, S

Su, WT

Duan, WN

Wu, Y

Xia, Z

HKU Scholars Hub

Protective effect of ginsenoside Rb1 against intestinal ischemia-reperfusion induced acute renal injury in mice

http://hub.hku.hk/bitstream/10722/196780/1/Content.pdf

Protective effect of ginsenoside Rb1 against intestinal ischemia-reperfusion induced acute renal injury in mice

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Directory of Open Access Journals

Crossref

HKU Scholars Hub