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research
Fabrication of uniform multi-compartment particles using microfludic electrospray technology for cell co-culture studies
Authors
Z Liu
HC Shum
Publication date
1 January 2013
Publisher
'AIP Publishing'
Doi
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on
PubMed
Abstract
Footnote in article: Paper submitted as part of the 3rd European Conference on Microfluidics ... 2012In this work, we demonstrate a robust and reliable approach to fabricate multi-compartment particles for cell co-culture studies. By taking advantage of the laminar flow within our microfluidic nozzle, multiple parallel streams of liquids flow towards the nozzle without significant mixing. Afterwards, the multiple parallel streams merge into a single stream, which is sprayed into air, forming monodisperse droplets under an electric field with a high field strength. The resultant multi-compartment droplets are subsequently cross-linked in a calcium chloride solution to form calcium alginate micro-particles with multiple compartments. Each compartment of the particles can be used for encapsulating different types of cells or biological cell factors. These hydrogel particles with cross-linked alginate chains show similarity in the physical and mechanical environment as the extracellular matrix of biological cells. Thus, the multi-compartment particles provide a promising platform for cell studies and co-culture of different cells. In our study, cells are encapsulated in the multi-compartment particles and the viability of cells is quantified using a fluorescence microscope after the cells are stained for a live/dead assay. The high cell viability after encapsulation indicates the cytocompatibility and feasibility of our technique. Our multi-compartment particles have great potential as a platform for studying cell-cell interactions as well as interactions of cells with extracellular factors. © 2013 AIP Publishing LLC.published_or_final_versio
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oai:hub.hku.hk:10722/190009
Last time updated on 01/06/2016
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info:doi/10.1063%2F1.4817769
Last time updated on 01/05/2021