Metabolite profiling using a novel QqQ linear ion trap mass spectrometer

Abstract

A novel hybrid RF/DC quadrupole-linear ion trap mass spectrometer (QqQ linear ion trap) was developed for analizing metabolite profiling. Primary hepatocyte incubations were used to demonstrate the utility of the QqQ linear ion trap mass spectrometer for metabolite profiling. High sensitivity in full scan mode MS, MS 2 and MS 3 modes, was provided by the ion trap scan functions of the QqQ linear ion trap. The flexibility to simultaneously perform quantitative and qualitative analysis was provided by MRM scan function. The experimental results show that seven major metabolites were observed during incubations.link_to_subscribed_fulltex