Poster Presentation: no. P90BACKGROUND: Significantly higher pharyngeal shedding of Epstein-Barr virus (EBV) is observed during HIV infection. Increased EBV shedding in pharynx is not affected even during highly active antiretroviral theyrapy (HAART). EBV positive monocyte populations have been shown to carry EBV to pharyngeal mucosa. Human cytokine profiles are often altered to facilitate herpes virus infection. Thus pharyngeal cytokine profiles may influence EBV reactivation and shedding during HIV infection. Our objective was to compare 37 pharyngeal cytokine profiles of HIV-seropositive patients who were or were not receiving HAART therapy …published_or_final_versio

Lee, MP

Li, P

Miller, C

Perera, RAPM

Samaranayake, LP

Tsang, PCS

BMC Proceedings

PubMed

RAPM Perera

PCS Tsang

C Miller

P Li

MP Lee

LP Samaranayake

Crossref

English

Identification of differential pharyngeal cytokine profiles during HIV infection

Springer - Publisher Connector

POSTER PRESENTATION Open Access
Identification of differential pharyngeal cytokine
profiles during HIV infection
RAPM Perera1*, PCS Tsang1, C Miller2, P Li3, MP Lee3, LP Samaranayake1
From Institut Pasteur International Network Annual Scientific Meeting
Hong Kong. 22-23 November 2010
Background
Significantly higher pharyngeal shedding of Epstein-Barr
virus (EBV) is observed during HIV infection. Increased
EBV shedding in pharynx is not affected even during
highly active antiretroviral theyrapy (HAART). EBV
positive monocyte populations have been shown to
carry EBV to pharyngeal mucosa. Human cytokine pro-
files are often altered to facilitate herpes virus infection.
Thus pharyngeal cytokine profiles may influence EBV
reactivation and shedding during HIV infection. Our
objective was to compare 37 pharyngeal cytokine pro-
files of HIV-seropositive patients who were or were not
receiving HAART therapy.
Methods
120 HIV positive volunteers under HAART and 72 HIV
patients not undergoing antiviral therapy were investi-
gated. All Volunteers with oral pathologies and smoking
were excluded. Pharyngeal secretions were collected
using standard procedures and analyzed for 37 cytokine
profiles using human cytokine profiler array panels and
ELISA. Cytokine interactome maps generated using
Ingenuity database.
Results
Proteome profiler arrays demonstrated differential cyto-
kine expression among HIV infected individual under
HAART, HIV infected individuals without HAART and
the healthy control. HIV group consisted of up regu-
lated C5a, G-CSF, CXCL1, soluble ICAM1, IL-1a,
IL-1b, IL1-Ra, IL-6, IL-8, IL-10, IL-12a, IL-13, IL-16, IL-
25, IL-23a, IL-27, IL-32a, CXCL10, CXCL11, CCL-13,
MIF, CCL3, CCL4, SERPIN-E1, CCL5, CXCL12, TNFa
and soluble TREM1 and down regulated soluble sCD40,
eGM-CSF, I-309, IFN-g, IL-17A, IL-2, IL-4, IL-5. From
these C5a, sTREM1, TNF alpha, CXCL12, CCL5, IL-17E,
IL-23, IL-32a, IL-16, CCL3, IL-6 showed significantly
higher expression levels in both HIV groups compared to
healthy group (P < 0.05). TGF b1 levels in HIV patients
undergoing HAART (0.5 +/-0.1 ng/ml) and without
HAART (0.4 +/-0.1 ng/ml) were significantly increased
when compared with the healthy control group (0.3 +/-
0.07 ng/ml) (P = 0.0001). TGF b1 levels had a significant
positive correlation with higher CD4 counts in the group
receiving HAART (P = 0.006). Cytokine interactome
mapping revealed significantly increased immune cell
trafficking in pharynx during HIV infection.
Conclusion
Pharyngeal TGF b1 levels are significantly increased
during HIV infection. As TGF b1 is a known trigger of
Epstein Barr viral lytic gene promoters, may influence
pharyngeal reactivation of Epstein-Barr virus. Addition-
ally, increased tendency for immune cell trafficking may
facilitate EBV positive monocyte trafficking towards
pharyngeal mucosa during HIV infection.
Author details
1The University of Hong Kong, Hong Kong SAR. 2University of Kentucky,
Lexington, KY 40536, USA. 3Queen Elizabeth Hospital, Hong Kong, Hong
Kong SAR.
Published: 10 January 2011
doi:10.1186/1753-6561-5-S1-P90
Cite this article as: Perera et al.: Identification of differential pharyngeal
cytokine profiles during HIV infection. BMC Proceedings 2011 5(Suppl 1):
P90.
1The University of Hong Kong, Hong Kong SAR
Full list of author information is available at the end of the article
Perera et al. BMC Proceedings 2011, 5(Suppl 1):P90
http://www.biomedcentral.com/1753-6561/5/S1/P90
© 2011 Perera et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.


HKU Scholars Hub

Title Identification of differential pharyngeal cytokine profiles duringHIV infectionAuthor(s) Perera, RAPM; Tsang, PCS; Miller, C; Li, P; Lee, MP;Samaranayake, LPCitationThe 2010 Institut Pasteur International Network Annual ScientificMeeting, Hong Kong, 22-23 November 2010. In BMCProceedings, 2011, v. 5 Suppl 1, no. P90Issued Date 2011URL http://hdl.handle.net/10722/164985Rights BMC Proceedings. Copyright © BioMed Central Ltd.POSTER PRESENTATION Open AccessIdentification of differential pharyngeal cytokineprofiles during HIV infectionRAPM Perera1*, PCS Tsang1, C Miller2, P Li3, MP Lee3, LP Samaranayake1From Institut Pasteur International Network Annual Scientific MeetingHong Kong. 22-23 November 2010BackgroundSignificantly higher pharyngeal shedding of Epstein-Barrvirus (EBV) is observed during HIV infection. IncreasedEBV shedding in pharynx is not affected even duringhighly active antiretroviral theyrapy (HAART). EBVpositive monocyte populations have been shown tocarry EBV to pharyngeal mucosa. Human cytokine pro-files are often altered to facilitate herpes virus infection.Thus pharyngeal cytokine profiles may influence EBVreactivation and shedding during HIV infection. Ourobjective was to compare 37 pharyngeal cytokine pro-files of HIV-seropositive patients who were or were notreceiving HAART therapy.Methods120 HIV positive volunteers under HAART and 72 HIVpatients not undergoing antiviral therapy were investi-gated. All Volunteers with oral pathologies and smokingwere excluded. Pharyngeal secretions were collectedusing standard procedures and analyzed for 37 cytokineprofiles using human cytokine profiler array panels andELISA. Cytokine interactome maps generated usingIngenuity database.ResultsProteome profiler arrays demonstrated differential cyto-kine expression among HIV infected individual underHAART, HIV infected individuals without HAART andthe healthy control. HIV group consisted of up regu-lated C5a, G-CSF, CXCL1, soluble ICAM1, IL-1a,IL-1b, IL1-Ra, IL-6, IL-8, IL-10, IL-12a, IL-13, IL-16, IL-25, IL-23a, IL-27, IL-32a, CXCL10, CXCL11, CCL-13,MIF, CCL3, CCL4, SERPIN-E1, CCL5, CXCL12, TNFaand soluble TREM1 and down regulated soluble sCD40,eGM-CSF, I-309, IFN-g, IL-17A, IL-2, IL-4, IL-5. Fromthese C5a, sTREM1, TNF alpha, CXCL12, CCL5, IL-17E,IL-23, IL-32a, IL-16, CCL3, IL-6 showed significantlyhigher expression levels in both HIV groups compared tohealthy group (P < 0.05). TGF b1 levels in HIV patientsundergoing HAART (0.5 +/-0.1 ng/ml) and withoutHAART (0.4 +/-0.1 ng/ml) were significantly increasedwhen compared with the healthy control group (0.3 +/-0.07 ng/ml) (P = 0.0001). TGF b1 levels had a significantpositive correlation with higher CD4 counts in the groupreceiving HAART (P = 0.006). Cytokine interactomemapping revealed significantly increased immune celltrafficking in pharynx during HIV infection.ConclusionPharyngeal TGF b1 levels are significantly increasedduring HIV infection. As TGF b1 is a known trigger ofEpstein Barr viral lytic gene promoters, may influencepharyngeal reactivation of Epstein-Barr virus. Addition-ally, increased tendency for immune cell trafficking mayfacilitate EBV positive monocyte trafficking towardspharyngeal mucosa during HIV infection.Author details1The University of Hong Kong, Hong Kong SAR. 2University of Kentucky,Lexington, KY 40536, USA. 3Queen Elizabeth Hospital, Hong Kong, HongKong SAR.Published: 10 January 2011doi:10.1186/1753-6561-5-S1-P90Cite this article as: Perera et al.: Identification of differential pharyngealcytokine profiles during HIV infection. BMC Proceedings 2011 5(Suppl 1):P90.1The University of Hong Kong, Hong Kong SARFull list of author information is available at the end of the articlePerera et al. BMC Proceedings 2011, 5(Suppl 1):P90http://www.biomedcentral.com/1753-6561/5/S1/P90© 2011 Perera et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative CommonsAttribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited.

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Identification of differential pharyngeal cytokine profiles during HIV infection

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