Session 8 - Epithelial cells, Infection, Carcinoma: abstract no. 55postprintThe 14th Biennial Symposium of the International Association for Research on Epstein-Barr Virus and Associated Diseases (EBV 2010), Birmingham, U.K., 4-7 September 2010

Cheung, AKL

Chua, D

Ko, JMY

Kwong, DLW

Lung, HL

Lung, ML

Stanbridge, EJ

Tsao, SW

Zabarovsky, ER

HKU Scholars Hub

TitleFunctional investigation of tumor and angiogenesis suppressivecandidate tumor suppressor, cysteine-rich intestinal protein 2 innasopharyngeal carcinomaAuthor(s) Cheung, AKL; Ko, JMY; Lung, HL; Stanbridge, EJ; Zabarovsky,ER; Kwong, DLW; Chua, D; Tsao, SW; Lung, MLCitationThe 14th Biennial Symposium of the International Associationfor Research on Epstein-Barr Virus and Associated Diseases(EBV 2010), Birmingham, UK., 4-7 September 2010.Issued Date 2010URL http://hdl.handle.net/10722/136034Rights Creative Commons: Attribution 3.0 Hong Kong LicenseEBV CONFERENCE 2010, BIRMINGHAM, UK    97                 FUNCTIONAL INVESTIGATION OF TUMOR AND ANGIOGENESIS SUPPRESSIVE CANDIDATE TUMOR SUPPRESSOR, CYSTEINE-RICH INTESTINE PROTEIN 2 IN NASOPHARYNGEAL CARCINOMA  Arthur Kwok Leung Cheung 1, Josephine Mun Yee Ko 1, Hong Lok Lung 1, Eric J. Stanbridge 2, Eugene R. Zabarovsky 3, Dora Li-wan Kwong 1, Daniel Chua 1, Sai Wah Tsao 4, and Maria Li Lung 1   1Department of Clinical Oncology and 4Anatomy, University of Hong Kong, Hong Kong (SAR), People’s Republic of China; 2Department of Microbiology and Molecular Genetics, University of California, Irvine, California 92697, USA; 3Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden   Abstract A novel candidate tumor suppressor gene (TSG), Cysteine-rich intestine protein 2 (CRIP2), was identified in NPC. This gene has previously been reported to be expressed in the heart endothelium; its possible relationship with tumor development is unknown. Therefore, its contribution and functional role in NPC was investigated. Methods/Principal findings: CRIP2 is down-regulated in 5/7 NPC cell lines and 42/60 (70%) patient biopsies. CRIP2 re-expression suppresses colony formation in vitro and tumor growth in vivo. Functional studies such as invasion, HUVEC tube formation, and in vivo matrigel plug angiogenesis assays were used to investigate CRIP2 gene function. Expression of CRIP2 inhibits angiogenesis both in vitro and in vivo. Using an angiogenesis protein array, several angiogenesis-related proteins were found to be down-regulated by CRIP2 re-expression. Analysis of conditioned media and gene expression in CRIP2-expressing clones validated protein array analysis results. Conclusions and Significance: An interesting candidate TSG, CRIP2, was identified. Functional studies confirm that CRIP2 can suppress tumor growth in vivo and inhibit angiogenesis. Angiogenesis is important for cancer development; inhibition of angiogenesis by CRIP2, which down-regulates the angiogenesis and VEGF signaling pathway proteins further confirms its importance in tumor development in NPC. CRIP2 is a potential candidate for future NPC therapeutic treatment.  Presenting author Email: mlilung@hku.hk                 Epithelial Cells, Infection, Carcinoma      Abstract 55 

Functional investigation of tumor and angiogenesis suppressive candidate tumor suppressor, cysteine-rich intestinal protein 2 in nasopharyngeal carcinoma

http://hub.hku.hk/bitstream/10722/136034/1/Abstract.pdf

Functional investigation of tumor and angiogenesis suppressive candidate tumor suppressor, cysteine-rich intestinal protein 2 in nasopharyngeal carcinoma

Abstract

Similar works

Full text

Available Versions

HKU Scholars Hub