Ho, YW

Lau, CS

Man, YK

Annals of the Rheumatic Diseases

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TitleEffects of Ganoderma Lucidium (Lingzhi) on proliferation andcytokine production of synovial fibroblasts from rheumatoidarthritis patientsAuthor(s) Ho, YW; Man, YK; Lau, CSCitation The 4th Annual European League Against RheumatismCongress, Lisbon, 18-21 June 2003, v. 62 n. Suppl 1Issued Date 2003URL http://hdl.handle.net/10722/57317Rights Creative Commons: Attribution 3.0 Hong Kong LicenseResult Content View2003] [AB0158] EFFECTS OF GANODERMA LUCIDIUM (LINGZHI) ON PROLIFERATION AND CYTOKINE PRODUCTION OF SYNOVIAL FIBROBLASTS FROM RHEUMATOID ARTHRITIS PATIENTS  Y.W. Ho 1, Y.K. Man 1, C.S. Lau 2 1Pharmacology, 2Medicine, The University of Hong Kong, Hong Kong, Hong Kong Special Administrative  Background: Ganoderma Lucidium (Lingzhi, LZ), a medicinal mushroom, has been suggested to have immunomodulatory functions. The major chemicals associated with these effects appear to be polysaccharides and triterpenes. Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by the proliferation of synovial fibroblasts that produce inflammatory cytokines and chemokines. It has been suggested LZ may alleviate the underlying pathology of RA. Objectives: To investigate the effects of Lingzhi (LZ) on unstimulated or IL-1β or LPS stimulated human RA synovial fibroblast proliferation and the production of IL-6, IL-8, IL-10, tumor necrosis factor-alpha (TNF-α) and monocyte chemoattractant protein-1 (MCP-1). Methods: Tissue samples were collected from patients with RA. The primary culture of synovial fibroblasts were incubated with LZ polysaccharides (hot water extract) for 24 hours at various concentrations. Proliferation of synovial fibroblasts was measured by thymidine incorporation. Toxicity of LZ on synovial fibroblasts was measured by MTT viability test. Cytokine levels in the culture supernatant were measured by enzyme-linked immunosorbent assay (ELISA). Results: LZ had a slight dose-dependent positive effect on unstimulated RA synovial fibroblasts on IL-6 and MCP-1 production. LZ also inhibited the production of IL-8 in a dose-dependent manner but it had no effect on IL-1β, IL-10 and TNF-α production. Following stimulation with IL-1β or LPS, LZ had moderate inhibitory effects on RA synovial fibroblast proliferation and IL-8 and MCP-1 production. No significant effects on IL-6 production was observed.  Percentage inhibition of IL-8 and MCP-1 by LZConcentration of LZ (μg/ml) Inhibition of IL-8 Inhibition of MCP-1IL-1 stimulated LPS stimulated IL-1 stimulated LPS stimulated50 7.9 ± 3.7 1.4 ± 8.9 8.2 ± 0.1* 16.7 ± 7.8125 17 ± 15.2 11.3 ± 7.5 9.6 ± 5.3 19.0 ± 1.1 *250 28.1 ± 13.5 28.2 ± 2.5 * 16.3 ± 14.9 # 30.3 ± 6.2 #Vs baseline *p<0.05, #p<0.1  baseline *p<0.05, #p<0.1 Conclusion: Lingzhi has slight to moderate positive effects on un-stimulated RA synovial fibroblasts in the production of IL-6 and MCP-1 but inhibits IL-8 production. Lingzhi inhibited LPS or IL-1β stimulated synovial fibroblast proliferation and IL-8 and MCP-1 production.  Rheumatoid arthritis Treatmenthttp://www.abstracts2view.com/eular/view.php?nu=EULAR03L1_2003AB0158&terms= [9/26/2008 3:44:50 PM]

Effects of Ganoderma Lucidium (Lingzhi) on proliferation and cytokine production of synovial fibroblasts from rheumatoid arthritis patients

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Effects of Ganoderma Lucidium (Lingzhi) on proliferation and cytokine production of synovial fibroblasts from rheumatoid arthritis patients

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