Airila-Mansson, S

Jin, LJ

Klinge, B

Soder, B

Soder, PO

Journal of Dental Research

HKU Scholars Hub

Titleα-D-glucosidase, α-β-D-galactosidases, and host cells ingingival crevicular fluid from subjects with aggressiveperiodontitisAuthor(s) Airila-Mansson, S; Soder, B; Jin, LJ; Klinge, B; Soder, POCitationThe 81st General Session and Exhibition of the InternationalAssociation for Dental Research, Goteborg, Sweden, 25-28 June2003. In Journal of Dental Research, 2003, v. 82 Sp Iss B,abstract no. 0582Issued Date 2003URL http://hdl.handle.net/10722/53272Rights Creative Commons: Attribution 3.0 Hong Kong License0582 α-D-Glucosidase, α-β-D-Galactosidases, and Host Cells in Gingival Crevicular Fluid from Subjects with Aggressive Periodontitis S. AIRILA-MÅNSSON1, B. SÖDER1, L.J. JIN2, B. KLINGE1, and P.-Ö. SÖDER1, 1 Karolinska Institutet, Huddinge, Sweden, 2 University of Hong Kong, Hong Kong  Objectives: The aim was to study α-D-glucosidase, α-β-D-galactosidases, and host cells in gingival crevicular fluid (GCF) from subjects with aggressive periodontitis (AgP-group). Methods: The participants were 20 subjects and 5 healthy controls. Gingival crevicular fluid (GCF) was collected from the deepest sites, 4 sites/subject. GCF was collected in an intracrevicular washing system. The protein content was assayed by a protein-staining method. The activity of α-D-glucosidase, α-β-D-galactosidases were determined by measuring the release of 4-methylumbelliferone at 450 nm. Cell counts were performed in a Bürker chamber. Analysis of Variance (ANOVA) and regression analysis were the statistical methods used. Results: There were significant differences in all clinical parameters (p<0.05-p<0.001) between the groups except number of remaining teeth. The number of mononuclear cells was significantly higher (p<0.05) in the AgP-group compared to controls. The total protein content was in the AgP-group mean 267.1(± 25.1SD) µg/ml and in the controls 111.4 (± 29.1SD) (p<0.01). There were statistically significant differences between AgP-group and controls in α-β-D-galactosidase activities (p <0.05). In the AgP-group there was a correlation between α-D-glucosidase activity and α-β-D-galactosidase activity (r = 0.81, p<0.001). Conclusions: α-D-Glycosidase and α-β-D-galactosidases in the GCF seem to react as sensitive biomarkers for subclinical changes that may later lead to clinical disease.    Seq #67 - Diagnostics: Periodontal Pathogens 11:00 AM-12:15 PM, Thursday, 26 June 2003 Svenska Massan Exhibition Hall B   Back to the Periodontal Research - Diagnosis / Epidemiology Program  Back to the 81st General Session of the International Association for Dental Research (June 25-28, 2003)     

α-D-glucosidase, α-β-D-galactosidases, and host cells in gingival crevicular fluid from subjects with aggressive periodontitis

http://hub.hku.hk/bitstream/10722/53272/1/Content.pdf

α-D-glucosidase, α-β-D-galactosidases, and host cells in gingival crevicular fluid from subjects with aggressive periodontitis

Abstract

Similar works

Full text

Available Versions

HKU Scholars Hub