Cheung, BPK

Dassanayake, RS

Jayatilake, JAMS

Samaranayake, LP

Samaranayake, YH

Yau, JYY

Journal of Dental Research

HKU Scholars Hub

Title The association of phospholipase C and virulence of C. albicansAuthor(s) Samaranayake, YH; Dassanayake, RS; Jayatilake, JAMS;Cheung, BPK; Yau, JYY; Samaranayake, LPCitationThe 19th Annual Scientific Meeting of the InternationalAssociation for Dental Research (Southeast Asia Division) &13th Annual Scientific Meeting of the Southeast AsiaAssociation for Dental Education, Koh Samui, 3-6 September2004. In Journal of Dental Research, 2004, v. 83  Sp Iss BIssued Date 2004URL http://hdl.handle.net/10722/53898Rights Creative Commons: Attribution 3.0 Hong Kong LicenseThe association of phospholipase C and virulence of C. albicans Y.H. SAMARANAYAKE1, R.S. DASSANAYAKE2, J.A.M.S. JAYATILAKE2, B.P.K. CHEUNG2, J.Y.Y. YAU2, and L.P. SAMARANAYAKE2, 1University of Hong Kong, Prince Philip Dental Hospital, Hong Kong, 2University of Hong Kong, Hong Kong  Although the significant roles played by phospholipase B in the initiation of cellular infection and, phospholipase D in the growth and dimorphic transition in C. albicans has been documented, the role of phospholipase C (PLC) is not yet well understood. Objective: To examine the role of phospholipase C in growth and tissue invasion of C. albicans. Methods: Phospholipase positive (two) and negative (six) C. albicans strains (detected by the egg-yolk-plate assay) were analysed for the regulation of PLC expression in YPD medium. The total RNA and genomic DNA was extracted using SV Total RNA Isolation System and Wizard Genomic DNA Purification Kit (Promega, Madison, USA). The transcriptional level of PLC gene was measured using RT-PCR. The primers used to amplify PLC gene expression (RNA and DNA) were; PLC1-1 & PLC-2 (PLC1-1 5'TTG TTC ACC GGA ATG TCA AA 3' (2187-2206); PLC1-2 5' CCC ATT GAA CAT CTT GAA CA 3' (3088-4990). The growth of yeast in RPMI was monitored using an automatic Spectrophotometer. Candidal virulence was examined using a tissue culture model based on reconstituted human oral epithelium (RHOE) and hyphal invasion detected by routine histopathology. Results: Significant differences in growth were observed between all C. albicans strains. Compared with the uninfected control there were marked histopathological alteration of the RHOE during the 48 hrs with PLC+ strains compared with PLC- C. albicans. Conclusion: Our results suggest that phospholipase C gene expression contributes to candidal tissue invasion, but not significantly to their growth in liquid media. (Supported by the Research Grants Council and the Committee of Research and Conference grants, University of Hong Kong, Hong Kong SAR, and the Outstanding Researcher Award of LPS).    Microbiology / Immunology and Infection Control  The Preliminary Program for Annual Scientific Meeting, 19th International Association for Dental Research-Southeast Asia Division and 13th Southeast Asia Association for Dental Education (September 3-6, 2004)  

The association of phospholipase C and virulence of C. albicans

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The association of phospholipase C and virulence of C. albicans

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