Cheung, GSP

Mak, YF

Journal of Dental Research

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Title Microbial-flora of root canals at the time of root filling and theoutcome of treatmentAuthor(s) Mak, YF; Cheung, GSPCitationThe 15th Annual Scientific Meeting of the InternationalAssociation for Dental Research (Southeast Asian Division),Taiwan, 2-4 October 2000. In Journal of Dental Research, 2001, v.80 n. 4, p. 1380, abstract no. P-4Issued Date 2001URL http://hdl.handle.net/10722/53870Rights Creative Commons: Attribution 3.0 Hong Kong License1380 Divisional Abstracts: Southeast Asian Division J Dent Res 80 (4) 20010-37 ~~~~Deterrlnadlon ofABO blood grouping uaing looth m#ADA Microbial-flora of root canals at the time of root filling and the outcome of0-37 ~~~~Supper'Jg kineramail for forensic kIderlllcatlen P- treatment.AuutoiEl Aae4e5 (~epL oOlOilop.FndiyfOatiby-UuYelkefladmee)Y.F. Mak*, G.S.P. Cheung (Conservative Dentistry, Faculty of Dentistry, TheDLnpt of Pwarneen Fwmnyof M*einie Uzdniv of bladoeeie) University ofHong Kong, Hong Kong SAR, China)Toothi tothe ost robuS end stabl part of VWthe numn body, end wtheefr potentially very usahu for bler*tylsevere, burned todles for foreneie purpowes. MHvwever co onel deno recorde mey not bte evegese or asinlera for The role of bacteria in the pathogenesis of pulpal and periapical diseases is wellsach purpose. ro etud Vie efleleincy end robuelnee of ASO blood grouping from toot meriel, gidected tDoot aelIM11e documented. The presence of bacteria in the root canal at the time of obturation may havegruigt rn a scoddo h146toothmOaWOWropPlee,aorcanoft 4 dw VW pp vf edW WO an impact on the outcome Of treatment. The presence study investigated the microflor a Ofwilt corleewer blod wmowed bnvrnedeily. mhe owher ofa ate 54 tear Vrat* eerIe, were stord et morn temprtureL root canal at the tune of root filling and the outcome of treatment. Samples were collected(2a+44C)foronOmorWs. in the student clinic from teeth undergoing root canal treatment. At the obturationThe reault aofrom enamel. the proporilon of corretlyADO blood group:ed tooth serrOple Yvwts earte Wee onl37 to 9% wi gWft* wn~ (p<001) W ftmderons pLipor cobV (boappointment, the root canal contentpomtmnt, wasrosampledconpriorasstoprootrirfillootgflandga culturedeto I t% ot VWe relsli vwer correct, end aterewe no elgnlteet diferene bebsee derine pul end cwe*o knrewael anaerobically. 31 tingle rooted teeth were sampled and bacterial growth was detected indW0*gcgw YM t excpoonof relibrly uneliebl blod groupin from enerneIEI ain0grior-eare teet for One~~wprabre~a toesarino ainiflest inluene in e mprlon wit ninmediat bodgupn tm 17 teeth (55%). A total of 15 species were recovered. 2 of which were strict anaerobese11r~acton. lHawoneram g"miderwgroun made telgnlerityleis$ ely*(p'O.01 for derdne end pul) toachiev correc and the remaining 13 were facultative anaerobic and aerobic organisms. All patientso wereblOod grouping from non.-cerlee tooth roatule in cornplaon with Imiinimelie blood groupn ite odUottoi or omW monoth invited for review in 6 months and 27 (response rate 87%) were examined.''13 teeth (48%)storege tmorntertenbakre. Fordentin and pup, onlaS70% ofbloodgroupig resub werecorct, forteovthwitheertleaciiclad.aiorphccrtri.NPEleulwerl aies pulp eppar to mwait cworrc blood grouping rrm tooth etrnlel (deoine end pul) signiicanOy leee were considered as successful unde titciia n aigahcciei.Nelgolfleartiferencein orrectblood groupingfrorigniicantdiffrenctwasdeteie&aoneerosete dewithern ou poriieeultureeanoe reeul cor4rvm toa emre"onela Isthmre11ie mateie for ADO blond 'ouing. mmw denselo , the time of obturation on the outcome Of treatment. The resul suggested that the presencetmM WO bwm mbem prwin M RISO c-11111161- 1 m mMmof bacteria at the time of root fillingt did not affect the outcome of root canal treatment in1115FM em m0111MC1ft. ro rwim m wnpymmawom ormawthe 6 months period. Long-term follow up is required to assess the impact of bacteria onuOrechoice topneale,te.t Mnier vlwtheee earlees poseelsielosedbeueed. the treatment outcome.The effect of rewening agents on in vitro recurrent caries. lothagarun A'; Tay FR';King"l~COMPARISON BETWEEN GTF 3-D MODELS PROVIDES POSSEItLITY-1 NM'; Wefel JSt; Pashley DH' ('University of Hong Kong, HKSAR; 'University of lows, P 5USA; 'Medical College of Georgia, USA) FOR VACCINE DEVELOPMENT' Y.-W. TSAI', Y.-Y. SHIAU. I.-S. CHIA, H.-C.This study examined the in vitro caries inhibiting potentisl of fluoride (FR) and non-fluoride-containing CHOU, Y.-C. LIAW, K.-L. LOU. (Graduate Institute of Oral Biology, College of Medicine,(NFR) rewetning agents that ore applied to acid-etched enamel and dentin before the one of water-free, dentinNainlTwnUivrtyTipiI0,AWN.adhesives. Twelve caries-free bicospids were divided into three groups. 2 a 3 x 1.5 mm cavities were prepared Ntoa awnUiest,Tie 0,TIA.on the menial and distal surfaces of each tooth, with half of the cavonurface margin in enamel and half incementum. In group I (control), One-Step (Bisco, Schamburg, USA) was applied without etching or rewetting Glucosyltransferases (GtfB/C/D) of S. mutans, a pathogen for human dental caries,agents. In group II, cavities were arid-etched, air-dried for 2s, rewetted with a NFR (Aqua-Prep, Bisco) for synthesize water-insoluble glucan through hydrolysis of sucrose. Genetic and biochemical20s, and then bonded with One-Step. Treatment for group III was similar to group II, except that a FR (Aqua-Prep F, Bisco) was used. Bonded cavities were restored with a non-fluoride-containing compost (&iel, approaches have identified several active sites of these enzymes, but no three-dimensionalBisco). Artificial carious lesions were induced in these specimens, from which 100±20 gm thick longitudinal structural evidence is yet available to elucidate the subdomain arirangement and molecularsections were subsequently prepared; yielding 16 specimens per group for evaluation with polarizing light mechanism of catalysis. Based on a combined sequence and secondary structure alignmentmicroscopy and microradiography. Representative sections were processed for transmission electronmicroscopy (TEM). Undemineralized ultrathin sections were examined unntained. Results: The outer lesion against known crystal structure of segments from closely related proteins, we propose herec thedepths (gm) were 116±5, 114±5 and 113±7, and the lesion areas ((tro) were 21,562±2,035, 14,966±1,819, 3-D models of the N-terminal domains essential for the sucrose binding and splitting in all10,829±2,302 for groups 1, 11 and Ill respectively. The differences were not statistically significant for lesiondepth (p>0.5, ANOVA, Duncan's test), but highly significant for lesion area (p<0.001). Wall lesions were three GTFs. Tim-barrel of (a/p),, structural characteristics is revealed and the structuralconsistently present in group I, while inhibition zones were invariably observed in group III. 87.5% of group correlation for two peptides Gtf-PI and Gtf-P2 (active sites) is described. Functional analysiswidth of 52.80±18gm. TEM showed that remnant dentin crystallites within the inhibition zones in group III acrigt h eonto fatbd gis I-Ib euigteezmtcatvt awere larger and denser than the corresponding wall lesions. They were of the same density and size slong the also been accomplished. Conclusion: Monoclonal antibody against Gtf-PI, which thensame lesion depth in group II specimens. It is hynothesized that a fluoride-containing rewettine sgent inhibitsrecurrent caries in vitro by altering so2atite dissolution. (Supported by University of Hong Kong CRC grant influences Gtf-P2, can be good candidates for developing vaccines to prevent human dental10202354) caries via disturbing GTF enzyme function. isupponed in pant by grant NSC 89-2314-B-002-258)The First Report ofCandida dublinienss from Human Root aries Lesions. SIiotomcSi\lol'F deorieooHitttirt PulpPupelllCultureres m ..inK.K. W. Tail. YP-2 Th is eoto add ulnessfo ua olCre ein.S P-6 "hn,(StIo o'IDetiristrv. Chung Shari Mtedical arid Derttal Cc'llege. Taichung.Dentistry. The University of Hong Kong. Hong Kong BAR, China). I""hc tser ofr,avs-tistnomtt cemsentts in resto'rative dentistrv hias iitcrrased ci'nsiderabl\v. dLii til thleitCfnrdrdyr drrhniriensis is a newly described fuingal species generally isolated from HIV-infected i\CrIIrsit CihCIitiril jIi'0p7eilten \itttterOLun stuidie' base resealed that contvenrtiontal "Iass-ttonorterpatients and considered an emerging opportunistic oral pathogen. However there are recent reports cLilictit litiii.milw~ Iltiti'ltl ii tir anr aqucetiisu environnrsentn. Hiosiever, thle 'ernrsnitivitt(If ill1.redof its carriage in healthy individuals as well as in non-oral sites. As little is known about the hutars 'Ii'il :ilk it, flstottle Iitis mist beers adeqtiatte studied The objective i'I Isis studs sirs tioprevalence of C'e,rdide dtibliniens.is in root caries lesions, we characterized 29 yeast isolates from i\,ittittie mise efetle tit'-I tltii'li s'ti (sulitsiai pilp cells, it' 1-113.3255 flut-irreiceiice cell priliferatiOri.root caries lesions for the presence of this potential pathogen.Attlo 9Cniaioae ee 'i ss-nh-iii ind sisii'tochondri i,atn i i' tsv mecte sed tot in\vestrr.s,te rise pa,thoirhilngicalobtained from 19 root caries lesions in elderly ethnic Chinese (12 patients, mean age 81.67 ± 6.30, 3 Clei,~tinl'I tlii'sdkiii si tilltitresI IrIitlstliplp ieron lu1.'riile sihiowed CvotrrtIc\ etlIecit oil rirititii Pull)males and 9 females) an described in our previous communication (Shen et al. J Dent Res 2000;79 ikItii a4i tsitisi tsadiardrie-eedetisrrieis(i Lntityi'special issue:395). Carrdida species wvere identified using the "germ tube' test, API 20C AUX yeast 1sd ctitimtss (ltsulrstisslelnrlhiiielIssitvmindttt" itstiuridentifiction kitwithannwlyupdaed identiication atabase bioMericu as. Mary-dEsoile 'ii .1 Inhtist iirlttilileitiil vnilieerisnitihens is iraIrisihisihirercsrrrcerrtt aatiss itInt iudse idispisdenrFrance) and growth at 455C for 48 bin (Jabra-Rizk et al.JChi, AMicrobiol 1999;37:1464). All yeasts masinitl II's :" II 'Iddnniriritio.il .newrrarionms sit'- irii-trsirussi 5 rnAl. tltsisride urisihiislbt 2'swere biotyped using the method of Williamson el al. (Microb.ios 1987;5t:195). Among 29 yeast issititiLl is' l 0e,i1iviisrii',r ameir ochorirale sriact\ tisuiV) .05cells pT ire tsroseig stuyecr if tioeide wsirisolates, three snere identified an Carrdida drrlslirriensis (10.34%), two were Carrdida glabrata isstrssstll sail -eLsee Isi ris exisrestle rqtes rd irtsr(6.90%~) and the remainder were Ca,rdida albicancs 1 (82.76%). The biotypes of all isolates varied hna tl,clsdeeddolteepSie(ts.feuny iddlailconsiderably.Our study reports, for the first time, the presence of Ca,odida dtrblirrie,rsis in root carieslesions. The presence of this rather virulent breast pathogen in root caries lesions of elderly isdisconcerting, as it may cause systemic morbidity in compromised situations. (Supported by aCRCG gmant of the University of Hong Kong, Hong Kongz BAR, China.)Inr-adInter-species Coaggregation of Bacterial Isolates from Root Surface SALIVARY CONCENTRATIONS OF CHLORIDE AND THIOCYANATELetins.S SHN0.L.PAMAANAY.KEand .K.YIP Faclty f~AFTER THE CHEWING OF MESWAK. R.A. JALILI, K. SUSHIL and L.A.Dentistry. The Uninversity of Hong Kong, Hong Kong SAR, China). SITI. (Faculty of Dentistry, University of Malaya, Kuala Lumpur, MALAYSIA).Bacterial coauggregation reactions between different species and the autoaggregation of the samespecies are associated with the initiation and development of dental plaque and biofilms ks no such The most widely used chewing stick is the meswak which is obtained from the plant,data is anailable on bacterial isolates from root caries lesions, we evaluated the coaggregation of 22 Salvadora persica that mainly grows in the Middle East. Meswak is believed to containdifferent bacrerral species comprising 10 different genera, from human root caries lesions. Bacteria substances of value for the prevention of caries and periodontal disease. T'he objctlive ofnvere isolated from 30 root caries lesions in elderly Chinese and identified using standard this study was to determine the effect chewing of two differently sized commerciallymicrobiological criteria (Shen ei crl. J Denti Re.v 2000:-79 special issue:395). Intra- and inter-species available meswak may have on levels of chloride and thiocyanate in whole saliva ascoaggregaruon wnas evaluated both by a qualitative visual scoring system (Cisar el al. Infect Immii,r opposed to the chewing of an inert material i.e. cotton roll. Twenty subjects participated1979:24 742) and a quantitarrve spectrophotometric assay (McIntire et al. I?!fect Imnrrrrr in this study. They were distributed into two groups (A and B). Subjects in both groups Ai978.2 1.978). The quantitative coaggregation assay we used proved to be a more sensitive method an Bfischwdome ak( mad10m imtrrsptvlyfloedbteiiiierrnediurrand 9diffeent pecie, and5) Fi.sabcterirnraitd.Bndf6rtherspecis.dTesersults meswk5cheingdcmpard toiottonrollinebohegropseA(33.6fmM l±5.78 d11.4 mMetml eexseco-utpl neacin ewentecteekio nuin atrilseis-.7-ndB(202mt.2,1.2 M±10) tpOtl lhug ihr eeso

Microbial-flora of root canals at the time of root filling and the outcome of treatment

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Microbial-flora of root canals at the time of root filling and the outcome of treatment

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