Samaranayake, LP

Tsang, PCS

Journal of Dental Research

HKU Scholars Hub

Title Predominant cultivable subgingival microbiota of HIV-infectedethnic ChineseAuthor(s) Tsang, PCS; Samaranayake, LPCitationThe 14th Annual Scientific Meeting of the InternationalAssociation for Dental Research (Southeast Asian Division), FortCanning Lodge, Singapore, 27-29 September 1999. In Journal ofDental Research, 2000, v. 79 n. 5, p. 1326, abstract no. 87Issued Date 2000URL http://hdl.handle.net/10722/53869Rights Creative Commons: Attribution 3.0 Hong Kong License1326 Divisional Abstracts: Southeast Asian Division J Dent Res 79 (5) 200081 of m asu~~a~for 3D na~ysisof doffpalate morhloy 2Jaw and tooth abnormalities detected on panoramic radiographs8 K.W..FOONG"A.SAND= SN.H.ONG .Y. WANG.A. KASSIM and C.W. WONG 8('Faculty of Denfistry and 2Faculty of Engineering, National University of Singapore) in New Zealand children aged 10-15 years. W.CHOLITGUL,* andB.K. DRUMMOND (Chulatongkomn University, Bangkok, Thailand andLandmark positioning on three-dimensional (3D) images, obtained fhroogh sorface laser scanning of a single Uiest fOao uei,NwZaadcleft palate plaster model, has been nalidated in a previous study by Foong at al. (1999). The aim of tee nvriyftg,Dudn,NwZan)present study is to determine the reliability of computer-derived measuremnents on 3D images of cleft palateTh aioftssud wsto sestepnrmcrdogpsofa fNwmodels wdth different paltafl morphology. Two duplicate plaster models of a complete unilateral cleft lip andTh ai ofhsstd wsto sestepnrmcrdorpsofagup fNwpalate infant, derived from alginate impressions taken at the third month prior to lip surgery and at the ninth Zealand children aged 10-15 years-of-age in order to determine the occurrence of jawmonth prior to palatal closure, were digistised wih the Cybarware 3030R-HIREZ surface laser scanner. Nine and tooth abnormalities. A survey of 1608 panoramic radiographs of a group of Newanthropological landmarks ware marked permanently on the models prior to scanning which served as visual Zealand children and adolescents aged 10 to 15 years (797 males and 81 1 females) wascues on the digitised image for landmark localisation. Each planter model was scanned ten times, and on-linelandmark digitisation was carried out for each of the ten scans to determine three linear and three angular carried out to determtne the occurrence of tooth and jaw abnormalitttes tn thts group whomeasurements. A single set of manual measurements for the linear and angular variables, verified by two were aftending the School of Dentistry clinics for treatment. 21% females and 17.3%investigators, was made with the original mfodels and served en a control for the computer-derived males, showed some abnormalities. Only seven (0.4%) radiographs showed bonymeasurements. Each laser-scanned image was calibrated against the original modal to eliminateabomlte.Te bnr liesdecd icued odyrdsubnes 3,measurement errors resulting from image magnification and distortion. Mea-suremnentsi were subjeldt bomlle. h bomltee cted inldd cndtrdsuracs ()independent f-lst with statistical significance set at p<0O01. The anterior cleft width, defined by the linear unidentified masses (2), bilateral cleft palate (1), dentigerous cyst (1).There were 879measurement AC-AC', showed the highest standard deviation for computer-derived measurements on both 30 teeth diagnosed with abnormalities on 331 radiographs. The proportion of teeth withimages at 10.5% and 20.6%, respectively. The standard deviations for the other two linear sod three angular abnormalities and the normal teeth was 1:51. The more common abnormalities detectedmeasurements were less than 1.5%. Computer-derived and manual hnear and angular measuremnents wereweemlo dteh.isnget,mssapdethndeth ihcr nhy paiacomparable for the nra-lip surgery plaster model l0.15opo0.81l. For the pre-palatal surgery model, theweem podteh, isnget,ms-aedethndeth ihcr nhy pai.difference beheeen manual and computer-derived measurements was statistically significant Ipo0t01l for only Hence we conclUde that this study has demonstrat-ed the value of goanoramicone angular variable, AC'-P'-P. Computer-derived linear and anuslar measurements on 30 imae ofra graRhy in detecting abnormalities in dental develoD2ment in particular. and wouldunilateral cleft lip and palate plaster modelsarer hiahly reseatabla ascent for the measurement definina the SuPRort the recommendations that are to be found in contemporary textbooks onanteriorletwichleft ewidth.rwr-Wh3RteIEtherfcCvbser3eareris a0acuraeH3stemfo surface detilaser tescanneranramisraangapaccuraten te sagstem f foradocumentina morsholog1caI chances of the cleft palate deformity. irnrove-ment is visual access for disiitsina oeiti etsr nteueo aoai aiqah oadi h igoi fdnalandmarks definina the anterior cfett width is needed. This study is supported by RP 950372 and RP950e25. development.ASuyoHedand Body Possture of Distal-End Edenstalous Patients. Effects' of Law-energy Laser Treatment on Enamel and Pentin Hardness. Cs.S83 P. SALMEE*, M. YINGTANOTAI, P. TAWEESAENGPANICH 84 HSIU, 'H SHI' and A YAP, (Faculty of Dentlietry, National University ot Singepore,(Dept. of Prosthodontics, Chulalongkorm University, Bangkok, Thailand) Singapore)There is now scientific evidence that laser treatment may inicrease the caries resistance of enamel.Apart from the importance of musculoskeletal system, the dental occlusion also takes part in Recently, a 98% protection against enamel mmnerl loss was reported with an artificial caries modelcontrol the postural balance. Since numerous teeth missing can cause the impainnent of muscle (Hsu end Wafst, 19981. The purpose of this study was Is inv.shgate the effect of the law-energyactivity, it might gradually effect the balance of posture caused by the difference of muscle tension C0u laser treatment on the hardness of enamel and dentin. Immediately after estrachon, a humanpremolar was dtstnfected, sectioned longifudinaNy, mounted, and polahad. Baseltne microhardnes,sbetween both sides. The objective of this study is to invesigate the relationship of the posture of readings (KHN) were taken every 200 gm along hen lines in enamel and destine with a Future-Techbody, head and neck in the patients who have posterior distal-end edentulous arch. Twenty subjects FM-7 digital microhardness fester (load = 100 gf, dwell time - 30 seconds). The central line ranwith full dentition and normnal class I occlusion were selected as a control group and fifteen subjects from the cusp tip to pulp horn, extending into dentin. The second (left) line was positioned 500 pmwith posterior distal-end edentulous participated in this study. The subjects were asked to stand in parallel to this line. After baseline readings, the tooth was then subiected to tour times of laserfront of the 5 cm2 mesh board for taking'the photograph of the frontal view (whole body). I-he body irradiation using the following parameters: power = 0.02 Watts, focal spot diameter = 1 mm, pulseaxis, head and neck axis, shoulders level, interpupillary tine were recorded to analyze the difference aithsie 010 seom sawa ryta thensiteprvosy= .9 /meaPsu-redawthintheasarmelne.Threbasreclndataof the axis compared to the vertical and horizontal axis.The results show that the heed and neck was analyzed with paired t-tests and showed no significant difference in hardness wihin 200 pmaxis of the posterior distal-end edentulous patients shift signif'icantly from the body axis than the divisions in enamel and dentin on both linies, before and after laser treatment (alt p > 0.05). Thecontrol group (1.971 and 0.82', respectively ,p<0.05). The axis of shoulder level compared to the hardness of enamel was significantly increased in the central and left lines with po0OtO1 (N=2t(,and p=0.031 (N=19), respectively. In dentin, laser treatment did net have a significant affect onhorizontal axis in the two groups were insignificantly different (1.270 and 1.350, respctively, P> hardniess in both tines (beth ps0O05). In conduasion. the low-eneray laser treatmenvt that inhibited0.05). It can he concluded from the study that a change of the head and body RLosture can be enamel demineralizafion also can increase the hardneas of enamel. The affset of laser treatment onexpected in Patients with Posterior distal-end edentulous. Supported by Dental research Fund, dentin hardness was not statisticalty sianificant.Faculty of Dentistry, Chulalongkom University85 Preliminary comparis onfa Loase-Induced Fluorescence end Mlcroradlography in 86 Factors associated wish unfavorable periodontal conditions in treated periodontal patients.85 valuationfa Enamel Demineralzastlon. CS HSU, CK Chlonh', YK Song, CK Chng, K.C. Ng*. LJ. Jin, W.K. Leung, EPF. CorbelKM Choy, TE Sash, and H SKI (Faculty at Dentistey, National University of Singapore, (Faculty of Dentistry, University of Hong Kong)Singapore)Carics diagnosis baa become morn criticul clan quality control emerged us the keystone or Health Carc ReCorm. A retrospective study was conducted so identify possible risk factors associated with further sooth loss and/orTraditional diagnostic systems used Cor caeies detection have Caced tremiendous challenges us the demand for higher relatively greaser proportions of sites with deep probing pocket depths (PPD) in treated periodontal patients.reliability and validity surCaced scrently. T'he search hor a better systcnsimnihod Cur caries diagnosis has become anThstdircueabalnatheocuinofcivprootlteay,aqsinarendalnclreimportant research issue. The purpose of this study was to invetigate the in-vinyo reliability and nalidity of a newly eafiainThestd pnlddartbiaslnets thre 0Coinclsio ofbjactive 4peidntale,6theffspy, agqedio4atr and yarclnialnedexcelptdwonwindowivcarresdtco.Tlet window teodCoth laswere sclecteetd with 211reNitanid-0.334st/cwt, vandithe 50.3±9.4 years), who showed initially favorable responses to periodontal therapy provided 3 to 12 yearseight windows servrd us ihe non-lasing conirvl (Hsu and Wefel, 19981. Allt hr tooth halves wnre then submitted to a previously. Data collected through the questionnaire included backgroued informnation, general health status,pH cycling model to create artificial caries-like lesions, as stated ie a previous study. All the tooth halves wier thee tobacco smoking, denture wearing habits, current orul hygiene habits, any follow-up dental care and furthercut longitudinally to obtain thin sections from each lesion Coe quantification of lesion depth and mineral toss by tooth loss after previous periodontal treatment. Clinical exarmination included teeth present and PPD at sinusing microwadiography IMRGI. After the MRG quantification ofCdemineralization, the lesions on teeth were Cartber sites around each tooth. Statistical analysis was performed using a general linear miodel. I11% of the subjectsa.ssessed hy five dental students using a new caries deiector lDiagnodentTm, KaVo, Germany). Each tooth half was were smiokers. 36% had primary education or tess, 53% had secondary school and 11% had tertiary. 258 teethfirst tested oare by each esamieer successively. This peroedure was repeated two more times before proceeding to in these 100 subjects had been loss, of which 195 )75.6%) were lost due to periodontal disease. Proportion ofthe nest tooth. This was done until all the tooth halves were tested. The reading of lusee-induced fluorescence ILIFI sites with PPD> 6mm varied among the subjects from 0 to 26.8% (rmean 3.04±4-58%). There wasabDindgpnodentsvwiabls use uscthedpnetvriabNVle.tabohenaminer and sequence oC measurementidwereth significant positive correlation between further tooth loss due to all reasons and age (pw0.00l), smioking-pack-significantly affeci tha reading oC LtF (boih p > 0.1151. Itits indicated that the lesions treated with uaser has tess years ),.nc0.002) and denture wearing (peo0.028) and a negative correlation with higher education levelsdemineralizaiion, and the contoxls base greater mineral toss lpa0.0011. For tesed lesions, the Pearson correlation (p=0.035). Tooth loss due to periodontal disease was also positively correlated with smoking-pack-yearscoefficient ( r ) was 0.k97 and 0.708. in the comparison of LIF osth enumeration of mineral loss sand lesion depth ie (p.c0.001) and age (p=O0.002). Percentage of sites with PPD> 6mm was significantly positively correlated withMEG, respectively (beth p<0.05I. For non-lased lesions, the r was 0.499 and 0.149. in the comparison of LIP with percentage of sites with plaque (.pe0.003) and denture wearing (p-0.O341. This studs confirms-that tobaccoenUmeration of mineral loss aed lesion depth in MEG, respectively (both p >0.05). It is suazaested is this study thal smnoking is a risk factor for, and that dentr wern is associate with, further tooth toss in traethe new non-invasive caries detector may he used validly to detect the iaciRient enamfel smooth caries, bat ant(for 98xjodotal pgaients. This project was supponted by thie University of Hong Kong (LURC and CRCG)-the advanced lesions. Diagenodentrel can also be reliably used for enumieration of LIP by different examiners.87 Predominant cultivable subgingival microbiota of HIV-isfected ethnic 88 Microbial colonization of spent Minocycline Stripo - A Preliminary Report.Chinese C.S.P. Tsang* & L.P. Samaranayake (Faculty of Dentistry, W,tK. LEUNG*, Q. SUN, J.Y.Y. YAU, Li. JIN, E.F. CORBET.University of Hong Kong) (Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China)This study investigated the colonization patters of oral microbes on Minocycline strips used as anThe obj.ectives of the study were to characterize the predominant cultivable subgingival adjunct in son-surgical periodontal therapy. Minocycline (1.4 mg/strip) and control strips weremicrobiota in a group of HIV-infected ethnic Chinese and to compare it with a HIV-free group. applied into all residual pockets (PD . 5 mm, > 4 pockets/subject) of adult periodontitis patients oneThe subgingival microflora of 21 ItIV-infected and II1 HtV-free individuals were studied using month after a coarse of sos-surgical periodontal therapy. The clinical experiment was conducted inthe direct microscopic and anaerobic culture methods. The major organisms found using phase a double-blind randomised parallel fashion. Strips were inserted into she pockets for 3 days eachcontrast microscopy were non-motile rods, in both HIV-infected and HIV-free groups (49.1% time on 2 occasions. Chlorhexidine mouthrinses were used during the week of strip placement.and 40.9%, respectively), followed by cocci (24.1% and 30.5%, respectively). Motile curved Spent strips were randomly recovered from 10 of the 32 participants as Days 3 and 6, and wererodis were almost thrcec times higher in the NWV-infected group. Gram slain examination yielded subjected to i) anaerobic culture using ETSA, ii) Coliforms culture using MacConkey agar, iii) yeastsimiarsl whrGrmngtvmdweetemjrmrhypobevdbtinteHVculture using Sabouraud's dextrose agar. The mean proportion of gram-positive species (% totalinfected and HIV-free groups (33.6% and 27.1%, respectively) followed by Gram-positive cocci cfu/strip) among all groups (teal vs control; 3 vs 6 days) ranges from 65-89% with gram-positive(I19.9% and 21.1I%, respectively). Is both groups, about 40% of the cultivable microflora were cocci being the most predominant species isolated (52-74%). The corresponding values for gram-strict anacrobes. Strictty anaerobic Gram-negative rods were the major bacterial type isolated negative rods were 10-29%. Minimal amounts of gram-negative cocci were recovered and one yeast(33.9% and 39.6% is healthy and diseased, respectively) followed by facultatively anaerobic was isolated on only one occasion. No difference could be found regarding the prevalence and

Predominant cultivable subgingival microbiota of HIV-infected ethnic Chinese

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Predominant cultivable subgingival microbiota of HIV-infected ethnic Chinese

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