Nair, GR

Samaranayake, LP

Journal of Dental Research

HKU Scholars Hub

Title Variations in germ-tube formation amongst oral Candidaalbicans isolates from HIV-infected and uninfected individualsAuthor(s) Nair, GR; Samaranayake, LPCitation11th Annual Scientific Meeting of the International Associationfor Dental Research (Southeast Asian Division), Jakarta,Indonesia, 4-5 October 1996, v. 76 n. 5, p. 1210Issued Date 1997URL http://hdl.handle.net/10722/53853Rights Creative Commons: Attribution 3.0 Hong Kong License1210 Divisional Abstracts: Souitheast Asian Division J Dent Res 76 (5) 1997Variations inL germ-tube formnation amiongst oral Candid-a albican.r isolates7 from lIRV-infected and uninfected individuals.NAIR RGe, SAMARANAYAKE LP (Oral Biology Unit, Faculty ofDentistry, The University ofHong Kong, Hong Kong).There is no data on germ-tube (GT) formation in C. albican.s isolates associated with H-IV-infection. Hence a total of 47 oral C. albicans isolates (29 from l{tV-infected and 18 fromuninfected individuals) was investigated using a previously described method using bovineserm and TC 199 medium as GT inducers. Briefly, 0.5 ml of C. albicans suspension (107cells/tnl) was incubated at 370C for 90 tnin with an equal volume of bovine serum. Onehundred Candida celUs were counted for GT formation using a haemocytometer. under 400xmagnification. In addition, a selec group of 6 isolates both from HI-V-infected and unsnfectedindividuas were pre-exposed to apo-lactoferrin (20 jigIiml) and lysozyme (20 and 50 oig/ml)followed by serum to evaluate their effect on GT induction. Amongst the media tested, serumproduced more GT compared with TC 199 medium (mean 25.2 - 35.7% and 23.0 - 26.2%/.respectively), irrespective of the source of C. albicezss. When the GT forming ability of C.albicans isolates from HIV-infected and uninfected individuals was compared, a marginallyhigh rats was obsered amongst the former group (p > 0.05). Whereas apo-lactoferrin (20x.g/ml) and lysozyne (20 .SWml) had no effect, lysozyme at a concentration of 50 isW/mlsuppressed the C. albicanw GT formiation. The forestoijn indicates tha serum is a better Gidcrcmoared wis1h TC 199 medium. And isolgate from tHV-infectedt individuLis and thoseuninfected have no- significantdfeec in GT formaing ability.Funsdedbyt Resesrech Graau Council of Hong Kong.5Subgingival microflora and periodontal conditions in outpatients at9 Chulalongkorn University. H.NAPAWONGDEE J.KUWATANASUCHATIand R.MAHANONDA'(Faculty cf Dentistry, Chtulalongkom University, Thailand).The aims of the present study were to evaluate the periodontal conditions using the CPITN and thenew loss of attachment index of WHO and atso to Investigate the presence of Porphyromonasgingivalis in subgingival plaque of the outpatients at the Fwacuty of Dentistry, ChulalongkomrUniversity. 268 patients Were examiAned. By using the CPITN only, It was found that more than 55%lof the patients under the age of 25 had gingivts and thei minority had periodontit3s. In contrast, morethan 60% of the older age group (s26 year old) had peniodontitis. The use Of the two indices togethewshow that most of the younger age group with 4-5 mm probing depth did not have attachment loss.On the other hands, the patients above the age of 35 generally showed the measurement ofattachment loss greater than probing depth. That might imply that the use of fte CPITN hMM=raeOIUDsems to overestimnate the orevalance and sevedtv of ceniodontal destruction andvice versa in teokdr oaruThirty samples of subgingival plaque were taken from either upper first or second molars (one sampleper one patient). Fifteen -samples were from the pariodontitis sites (CPITN=4), whfile the rest werefrom the healthy sites (CPITN-0). P.plnghvais was identified by cutturing technique, Gram stairsmear and the illumination test under UV light (385nm). P.gingivalis was detected in 86.67% of thesites with advainced periodonbtiis, whie none was found in the healthy sites. The proportion of thisorganism to anaerobic bacteria in the diseased group ranged 0.18-18.67% with median of 4122%,hence supporting the role of P.ogovais in advanced oedodonfits In the T'hai popsulation.+IE POTBIrIAL EFFECT OF CATucHIN INa TSEA LEAkF ON SiFITEPI3l5AJ ,.JTAN6ii R. R. OEWEN' (PEDOOONTC DEPT. FACT3,LY OF 00(ISlWPADJADJARAN UNI1VEBS5TY, gANOiUNl-INDOCtIA)The objective of the study vm to determine the minimal ooncentration (NEC) ofcatechin In ten that could inhibit the growth of Streptococcus, mutans. This bacteriais predominant in ceriese formation. Based on the 141C it could be decided a ueeddilution cocanmtration (UDC) of catechin in tea.The ample was scraping of caries lesion taken froe children who visitedPesdodontic Clinic - The Faculty of Dentistry PadJadjaran University. The extrac-tion of catechin from green ten was done in The Ten Research Center Laboratory inQatisng-Ondonesia.A serial diluion method of NOCLS (leSM) wan. used to determine the minimalinhilbitnry concentration of catechin to Streptococcus mutana.The microscopic examination and the Identification of the cultures indicatedthat Streptococcus mutans is detected predominantly in caries. The serial dilu-tion test showed that the minimal inhibitory concentration (MOO) of catechin was0.6 mg/al.The statistical analysis using nTwo Ways Analysis of Variance followed byIlul tiple Comparison ahowed that the number of Streptococcus eutans decreasedsignificantly in 0.5 m agconcntration of catechin with the optimal contact time of3 to 5minuate. It ia anS dthsat labe used as a owth rle Aued dilutiulcusnoentration CUDCC of catechins In ten is 2 no/'ml (4j MOc). This study was sup-ptorted by The Sitf - Ace, Loan Nto. 1013-INtO.'1Strngh analysis of tixaklioe of usgilttal split ostoaotoy. B. ANUCUL5, P.D. WAMilt 3.13 E. LEMIONS and C. VLACHOS (Mahidot Univeraity, BIKKThaiIand andIUAB,Alabans,~USA)six fresh frozen human mandible wer used as three dietnotionaltiessig~specimcens. Bilatera sagilta spliasoensonmics were perfonned and 5-mm. advsncenments wecre nuade The specimens wver divided intoplate-fixed group and sicrew-fixd group. All specimens were subjected to repe-ated cmposnpresl loads of22.27N bv using Instron machine. Parameters of esrengh were analyzed bry elastic deformation, stiffnesratio, permanent deformation and breaking load. Data manalsis wvere done by means of two-sidematched pair t-lest. The mecat elaslic defonmation under 22.27N wan 0.36±.4 mmn in plate group and0.24±0.24 nmm in screw group which were not statistically different (0.60'tp<0.t0).Ther was statisticaldifferences betwen mean etifi&sem ratio of the two group (0.40cp<0.60t while tie plate group was1232.58±153.24 N/mm. the screw group was 342.91+195.89 N!nmn. The mean load for permanentdeformation %%vre statistically differen betweea two groups (p<0.000I).The plate. grouap was 16.43+16.39N, whereas the screw group was 34+5.79 N. The mean breejing load of tIhe plate group was 2-3.545.59N whil the screw group wAs 63.33+20.38 N. It was significanily three tiene higher than that of the plategroutp (0.0l'-p-- 0.05. lUis concluded that the bicosicat screw f esssai ±esss .bgl.e.osLsnVgngq.qkjVjis4e..f iMs.slsji)enitknQr aIosed load to cause fix-ation failure in the hicortica screw,ssiemJtan th_e.in.incories.pist nyi.mMinImum lahibltory Concentration (MIC) of Antibacterial Agents Agaiins-t8 Carliognlc OrgansmsMG Botelhoe & LP Samaranayake. Prince Philip Dental Hospital, Hong KongWhile some resarch hasinvestigated the MiCof antibacterial agents against bacteria associatedwith plaque, there has been little on the MICa of bacteria specifically isolated from cariouslesions. A total of thirty one bacteria: streptococci (9), lactobacilli (10) and actinoinyce (12);isolated from carious lesions were tested an to their minimum inhibitofy concentration (MIC)against eight antibacterial mouthwash agents. The antibacterial agents were: chlorhexiddinediacetate, chlorhexidine dihydrochloride, chlofhessdine' guconate, benzakoniium chloride,cetrimide, cetylpyridinium chloride, thymol and sodium hwohoie sn naceptedprotocol, serial dilutions of the antibaectrial ageats were prprdinmco itepate inconcentrations from 64.ug11n to 0.0625 AIegns. An inocalum othtetrgnseqvanttoi0sr5 colony forming units pr millilitre was dispensed into a mroelcontainin thioglycolatemedia and antibacterial agent. The tray was then incubated for 48 hours at 35'C. After this time,the lowest concentration at which no growth occurred was recrded to be the MIC.MTyntol andsodium hypochloTite did not show any antibacterial activty at the concentrations tested. TheMIC range for all the microorganism~s was 8.0 to 0.125 peg/mI. There was no statisticallysignificant difference between the MICa of the three chslorhexiddines and the remaining 3antibacterial agents. The reults from this atudy nicate- that while *isyold and sodiumnhvwlrt have. limikited antmicmbial effe,'A antibanierial seants commonnv nsed inmouthwashes bave similar MClvlaais sieicbacteriaBacterial nxorphotyp of SupragingVAl plaique in Chinese subjects.1 K.Y. ZEE', L.P. SAMARANAYAXJE (Department of Periodontology & Public, Healthand Oral Biology Unit, Faculty of Dentistry, The University of Hong Kong)The aim of this study was to estimate the percentag distnbiution of different bacteral morphptypes ofsupragingival plaque in Chinese subJects by using the exermental gingivitis modeL Seve health dentalstudents were recruied froms Prince Philip Denta Hospital. All the subjects were provided with oncoe-a-wveek professonal prophsylaxis for 3 weeks in order to ensure giagival health. In the fourth week, afterprophyhxis, the aubjectsabegan a 21-day period without any mechanical or chemnical plaque control. Plaquealong thebuccal gingival msarginof 16, 15, 14, 24, 25, 26 weireremwoedon Day 1, 3,5, 7, 14,21 respec-tivelywi6th aster*iecrte and dispersed onto a drop ofdistiledwaterplacedon anucroscopic slide. Gramstainted snmeasrwere prepared for fight nicroscoy. At Il000Xmas'fcton, the singlecel seenwasdass-tied as Gram-positive or Gram-neative cordi, rod, filament, fiusiform organism, spirilla and spiroobwtte AdiffeetalO count of 200 organisms from 3-6 fields in the microscopewere performed. The figre obtainedforeachgroupofraicroorganismsweretlenextpressedas.aperctttaeofthetota nun*erofbwctsiacounted.Gingivl erevicular flud (GCF) flow were measured from 13-23 during the 21 days by umang Periotronemachine Atota of42 esmersrwere obtained and eammined. R.eultsashowed GCFloawiiscresse during the21-day period. Om-ve microorganisms were the predominant type of biacteria throuhout the 21 days (66-93%) while Gns+ve bacteria only constituted around (5-22%). The proportion of the shaped bacteria in-creased with the plaque age. Ths is inl conntra to result frm tuesuiathe same gnhool inCacsanM b n nwihC2"bctrawr h eoiatmWrl Furthe studies in theChinese population are needed to confirm or refute these fisdinpa12 Reltion of Plasma Albumin Level to Calculus12 epoivemi.Y. ndonesia,Z nstitA t PArtassan oorPuamlitbang Gizi, Indlonesia).Previous studies have ahown that a group of proline ricsproteins in human saliva can act as inhibitors of calciumphshtprecipitation in dental calculus formation. Sincacme of native Indonesiause stil consume diet werth a loneprotein level nowadays, and the fact that mesy Indonesisamhave high score of calculus index, the purpose of thispreliminary study wass to investigate thke cosrelation betweenplasna albumin level ( as a body protein index ) endcalculus deposit on the tooth surface. 34 people aged 22-50years, in Bogor, Indonesia, were subjected in this study.Plasma albumin level nwas measured usingj perohtceterIcalculus index wee used to assess calpulus deposit. Theresult showead that there was significant snegativecorrelation between the plasma albumin level and calculusindex ( r-- 0.43, P 0.01 ). It s nuh tathelone14 Blonhesmlcax Differences Between Eadochoadral Bone and IntramembranoesA. TWrTIMY, A.M. RABIE antI D. SHUM5(Departmsent of Children'sDentistry and Orthodontics, Departisset of Biochesnistry ,' The UnIverity ofHong Kong.)In a qualitative study, we reported that composite itmrmemnbrassou bone induced more new bonethan composte cstdocboontrl bone. In order to elucidate the factors behind the enhanced osteoenicactivity of itaebaosbone, biochemical analysis of the extraellular matrices of both typeof bone was carried out specifically for the presene of an angiogenti inhibitor. Endochondral bone(femur) and intramembranous bone (mandibles and paricta bone) were harvested fromn 3-4 monthold New Zealand white rabbits. T'he bone was cleuaned. defattod, dried and pulverised (particle tire;60.23mm) before being tdemineralized in O.5M HCI. Protein wan extracted dissoiatively using 4MGuHCI and ultrafiltration was used to give a 10-lOOkDa molecular weight range. HepariSepharose Chromatography further separated the proteisn before they were rns on 15% SDSpolyacrylamide gels undcr reducing conditions. Preliminary studies have showna that there isdiffernce in banding nastrn between fth two bowe tpe with rFssita soina the oresstecof a28-29kDa--" 1- I-.nrsti i hesoasBosMe -tO5Oe- oMLalA RmlelmJ.-A - U1Jn55fcssLthe guivalenLotcSiMKemranosQ boine..fractionl. Further investigations are being carried out todetermine the nanar of this protein in order to establish whether or not it is an angiogenic isnhibitor.This study was supported.byRCG Grant.#-312L2,.51/6425, Uriversity.f Hong Kong.a dMMI2qkDa protein in the bCodu bound frwtion of bome RMWM and absence- A.S.- .2- a &. -.0 46I.-

Variations in germ-tube formation amongst oral Candida albicans isolates from HIV-infected and uninfected individuals

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Variations in germ-tube formation amongst oral Candida albicans isolates from HIV-infected and uninfected individuals

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