Cheung, BPK

Corbet, EF

Leung, WK

Ngai, VKS

Tsang, PWK

Yau, JYY

Journal of Dental Research

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Title Actinobacillus actinomycetemcomitans isolated from youngChinese adults with aggressive periodontitisAuthor(s) Leung, WK; Ngai, VKS; Yau, JYY; Cheung, BPK; Tsang, PWK;Corbet, EFCitationThe 19th Annual Scientific Meeting of the InternationalAssociation for Dental Research (Southeast Asia Division), KohSamui, Thailand, 3-6 September 2004. In Journal of DentalResearch, 2004, v. 83 Sp Iss BIssued Date 2004URL http://hdl.handle.net/10722/53754Rights Creative Commons: Attribution 3.0 Hong Kong LicenseActinobacillus actinomycetemcomitans isolated from young Chinese adults with aggressive periodontitis W.K. LEUNG1, V.K.S. NGAI2, J.Y.Y. YAU3, B.P.K. CHEUNG3, P.W.K. TSANG4, and E.F. CORBET2, 1University of Hong Kong, Hong Kong SAR, China, 2University of Hong Kong, Hong Kong, 3University of Hong Kong, Oral Bio-Sciences, China, 4Chinese University of Hong Kong, Hong Kong SAR, China  Objectives: To characterize A. actinomycetemcomitans isolates from young Chinese aggressive periodontitis patients. Methods: Subgingival plaque samples (2/subject) were collected from untreated diseased subjects < 25 yr (n = 9, 21.1 ± 1.6 yr) and age- and sex-matched periodontitis-free controls (n = 47, 22.0 ± 1.1 yr). Selective (TSBV agar) and anaerobic culture were used. The serotype, leukotoxin gene (ltx) operon promoter, and the cytolethal distending toxin (cdt) genes complex of the A. actinomycetemcomitans isolates were studied. Results: Diseased subjects had significantly higher full-mouth bleeding scores (p=0.002) and total viable counts (7.2x106 vs 2.1x105 c.f.u., p<0.005). A. actinomycetemcomitans was isolated from 67%/56% or 6%/4% of diseased or control subjects/sites, respectively (p<0.001). The proportion of A. actinomycetemcomitans isolatable from both diseased and periodontitis-free was low (0.7% vs 0.1%, p<0.02). Among the 10 disease and 4 control A. actinomycetemcomitans isolates characterized, 8 were serotype c, 3 serotype f, 2 serotype a and 1 serotype b, and all possessed 652-like ltx gene promoter; furthermore all but one from diseased patients had intact cdtABC genes. Conclusion: This study confirmed increased prevalence and quantity of A. actinomycetemcomitans in aggressive periodontitis Chinese patients. The ltx promoter and cdt characteristics of the A. actinomycetemcomitans isolates, however, were similar among the diseased and control groups.    Microbiology / Immunology and Infection Control  The Preliminary Program for Annual Scientific Meeting, 19th International Association for Dental Research-Southeast Asia Division and 13th Southeast Asia Association for Dental Education (September 3-6, 2004)  

Actinobacillus actinomycetemcomitans isolated from young Chinese adults with aggressive periodontitis

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Actinobacillus actinomycetemcomitans isolated from young Chinese adults with aggressive periodontitis

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