Fernando, PHP

Panagoda, GJ

Samaranayake, LP

Journal of Dental Research

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TitleThe relationship between the acid and alkaline phosphataseactivity and the adherence of Candida parapsilosis to humanbuccal epithelial cellsAuthor(s) Samaranayake, LP; Fernando, PHP; Panagoda, GJCitationThe 47th Annual Meeting of the Japanese Association for DentalResearch, Kobe, Japan, 27-28 November 1999. In Journal ofDental Research, 2000, v. 79 n. 5, p. 1254, abstract no. 155Issued Date 2000URL http://hdl.handle.net/10722/53927Rights Creative Commons: Attribution 3.0 Hong Kong License1254 Divisional Abstracts: Japanese Division J Dent Res 79 (5) 20001 53 Expression of the Cell-Surface Heparan Sulfate Proteoglycan mRNA in 1 54 VAMP2-containing Complex at Secretory Granules in Parotid AcinarMonkey Submandibular Gland. E. YAMAGATA, A. KAMADA and Cells. J. YOSHIGAKIP, Y. DOHKE, M. HARA-YOKOYAMA, S.T. SAKAKI (Osaka Dental University, Osaka, Japan) FURUYAMA, H. SUGIYA (Dept. Physiol., Nihon Univ. Sch. ofCell-surface heparan sulfate proteoglycans belong primarily to two families of molecules, Dentistry at Matsudo, Chiba, Japan):synidecana and glypicanis, that differ significantly in core protein domain structure. They Amylase release from parotid acinar cells is mainly regulated by accumulation ofhave been shown to participate in both matrix recognition and growth factor binding intracellular cAMP. We previously reported that VAMP2, one of the SNAREand thus may participate in cell regulation. We investigated the mRNA expression of proteins is specifically localized at secretory granules in rat parotid acinar cells andcell-surface heparan sulfate proteoglycans in adult femalemonkey (Macaca fascicularis) has an essential role in cAMP-regulated amylase secretion. We also found thatsubmandibular glands using the RT-PCR technique. Agarose get electrophoresis of the VAMP2 makes complex with some unidentified protein(s) at secretory granules inPCR products of the cDNA generated frxm RNA wss carried out to demonstrate the the resting state. In the present study, we investigated whether the VAMP2-expression of mRNA in syndecan- l, syndecan-2, syndecan-4 and glypican in this study. containing complex is pre-existing SNARE complex before priming. AlthoughIn order to compare the mfRNA expression level among the cell-surface heparan sulfate botulinum neurotoxin B (BNT-B) is a specific protease of VAMP2, VAMP2 in theproteoglycans, we measured changes in the relative intensity of PCR products with SNARE complex is known to be resistant to BNT-B. We incubated solubilizedincreasing thermnal cycle number. The results demonstrated that the -expression levels granule membrane with activated BNT-B and performed immunoblotting analysiswere syndecan-4, syndecan-lI and syndecan-2, glypican in descending order. Hence, it with anti-VAMP2 antibody. As a result, VAMP2 in solubilized granule membranewas indicated that the control of the expression pattems of the cell-surface wss efficiently cleaved by B3NT-B. This result suggsests that the VAMP2-containingproteoglycans may regulate the cellular function and behavior in the submandibular complex is not pre-existinez SNARE, complex. This study was supported in part by aglan. Ths sudy asuppotedin prt y a rant(097191) fom te SientficGrant-in-aid for Scientific Research (No. 09771550) from the Ministry of Education,Research Fund of the Japanese Ministry of Education.SceeanCutrofJp.1 55 The relationship between the acid and alkaline phosphatase activity and the adherence 1 56 Oral infectivity of Candida species in a healthy and immunocompromised animal miodrl Y.of Candida parapsilosis to human buccal epithelial cells. *L P. SAMARANAYAKE,'I H. SAMARANAYAKE and L. P. SAMARANAYAKE (Oral Bin-sciences, Faculty ofP. H. P. FERNANDO2 & G. J. PANAGODA2 (t Faculty of Dentistry, University of Dentistry, University of Hong Kong, Hong Kong)Hong Kong, Hong Kong, 2 University of Peradeniya, Sri Lanka) Little is known of the pathogenic potential of different Candida species in healthy and compromined animalmodels. Therefore we investigated the oral cotonisation and infectivity of C. albirans and C. krusei inCandida parapsilosis is an emerging fungal pathogen implicated in many diseases, especially in healthy and immunocompromised Sprague-Dawley rats. A total of 15 rats, were allocated into three groupscompromised hosts. Candidal colonization and infection depends on their initial ability to adhere to and were fed on a carbohydrate-rich and a tetracycline laced diet. These animals were orally inoculatedhost surfaces, which in tarn depends upon the host and the yeast cell wall components and the related using standard suspensions of clinical isolates of Candida. Fungal colonisation was then assessed bybiochemical mechanisms. Therefore we examined the potential pathogenic traits of 24 C. swabbing the full mouth in a standard manner at regular intervals and, infectivity was evaluated by routineparapsilosis isolates, from both superficial and deep infections by evaluating and correlating their histopatbologic techniques (Samaranayake et at., J Med Mirrobiol 1994; 41: 250-8). After a three weekintracellular phosphatase activity measured with para-nitrophenyl phosphate (Onishi et at. 1. Biol. period of intermittent oral inoculation of healthy rars, both species demonstrated variable oral colonisationChrem. 1979; 254: 11943.52) and their adhesion to human buccal epithelial cells (samarinayake et at. speiesinuchtedoandidCalbinfctins darigiing nthis praeriod. 0Subequnt Cimmunsupesioneoftheles ran,itbyJ Med Microbiol 1912; t5: 511-517) Significant intraspecies differences were seen in both the intramuscular cyclophosphamide initiated C. albicans infection of the dorsal lingual mucosa especiallyalkaline and acid phosphatase activity as well as in their adhesion to buccal epithetial cells (p < around the conical papillac after a few weeks in all animals in this group white similar lesions due to C.0.0001). Farther the acid phosphatase activity of the superficial isolates was significantly greater krusei were seen in only 37% of the Innter group. Histological changes resembling mucosal candidosis were(152%) than the systemic isolates (p = 0.0352). A highly significant positive coffelation was also observed in the lingual mucosa of rats infected with both Candida spp. Further, both species peoduced fungalestablished between the yeast adhesion to buccal epithelial cells and, both the acid (r = 0.18, p <hyphae that penetrated the epithelium, although C. albicans hyphae tended to be relatively more profuse. in0.0001) and alkaline (r = 0.9, p < 0.0001) phosphatase activity. These relationships- described for the conclusion these resuls cgjnfirm the clinical observation that C. kdAsei_ thoughancacouncmmensut isfirst time- imply that the alkaline and acid phosphattises of Candida speies may play a subst2antiveal ota%omit nivsy ahon nc n ute-teS nmlnierote in poetating their virulence- useful for further studies on the ralafive infectivity of differeut randida tpCeriesSuapportedby theCRCofthe Uiversity o Hong Kon and the RC grants.Supported by the CRCG of the University of Hong Kong (grant nos. 335/268/0004 and 335/268/0006).S. Biochemical Approach to Synovial Fluid associated with S-2 CarnicalAssessment forJoint Effusionofte MsJTemporomandibular Joint Disorders fDnityadK KfAYASHIB ( Department of Cra Radiclogy, TsurumiiTakanori SHIBATA1 and Masao NAGUM02(DepartmetoDnisrad Urniversity Schocl ofiDental Medtidne,Yokoharna,Japan)Oral Surgery, School of Medicine, Yamagata Universityl,Second Department I'h pups of this presentation was to find out a possible correlation between theof Oral & Maxillofacial Surgery,School of Dentistry,Showa University2,Japan) M mg ftetmooadblrjit(M sfeigfo ottefso nRecent improvements in techniques for detecting trace amounts of biologic molecules in M iagofthetesigsmptoromndiuiajointpannditvrnal suferinge fomjont.92Weffusionansmall volume of synovial rtuid have lead to analysis on various inflammatory and cartil- the exiostn sympetomtatic jointpainandionteral doeryangement.eI 1992, Wesntessonage degradation markers in the diseased temporomandibular joint(TMJ). Thentihe first HandvBroussgetdta hjoint effusionwscnieeocreasedoshery assmeofatedswthoFcorlpaininternational symposium on TMJ synovial fluid analysis, which was named "Biochemical Hwvr on fuini osdrdt orlt te oefcos o.Fr 2Changes in Synovial Fluid Associated With Pathology of the Temporomandibular joint", TMJs with dinticali symptonis of the TMJ disorders, sagittal and coronal T2* and T2was held in 75th general session of the international Association for Dental research at weighted images were obtainved The imiage ftnding for joint effusion were correlatedOrlando in 1997. After this symposium, in Japan, a lot of newly research groups have with disc positioni, disc configulation and pain. The cases were dassified into threegrown in this field. They have been focusing and analyzing on various molecules in TMJ groups by the extent of anterior disc displacement. The TM.Js with anterior discsynovial fluid e.g. interleukin 1- ,tumnor necrosis factor- o, Interferon- -r. matrix displasement showed a higher frequency of joint effusion than the TN-ts with themetalloproteinase 1 and 3, tissue inhibitor matrix metaloproteinase I and 3 As inflamm- disc normally positioned. (p<0.05) Among the joints with the anterior discatory markers and Chondroitin sulfate, Hyaluronic acid, Keratan sulfate, procollagen 11 displacement without reductioni, the biconicave group showed a higher frequency ofpropeptide, pyridinoline and deoxypyrodinolitne as cartilage degradation markers. joint effusion than the disc deformity group. (p<0.05 by chi- square test) InThe purpose of this symposium is to report on the current state of knowledge regarding conclusion, the MR imae evidence for ioint effusion in the TMJ was assodated withthe biochemical chanaes in the synovial fluid associated with TisI disorders, to discuss the presence of disc displacement . disc configulation and TM] pain, for it wastheir role in the jloit pathologty, to provide data regarding the possibility as molecular unspefc±itpi.sgss.markers of disease, to assess the advance of research in this field duringt the last twoyears, and to accelerate the advance of research in this field.S-3 Analysis of proinflamnmatory mediators in synovial fluids of the TMJ. S-4 Assay of cartilage metabolite in synovial fluid of TMJT. TAKAHASHI* (Division of Dentistry and Oral Surgery, Akita Kenji KAKUDO' ( Second Deparment of Oral and Maxillofacia Surgery,University School of Medicine, Akita, Japan) OaaDna nvriy aaVarious inflammatory mediators, including arachidonic acid metabolites, cytokinesocarf h OehnsakaDenalrUivaeriy Jegapatin)o eprmniuajit(M) eivsiaeglucosaminoglycan components, protenases, neuropeptides. and free radicals are foundin SF fromTocaiytemhnssofarlgedrdtonfsmpoadburonrTM)wenvtgtdpatients with TMD. In this study, to investigate how these inediators are involved in the pathology of the molecular size profile of hyaluronic arid ( HA ) and the activity of its degradation enzyme, N-acetylsynovitis and cartilaginous degeneration and in clinical features such as pain, a proinflammator-y-,3 -glucosaminidase (NAG ), examined in the synovial fluid (SF) collected from patients with intemalrytokine. IL-I P and a gasseous free radical, nitric oxide (NO). were analyzed using synovial lavage derangement of TMJ ( ID group ) or osteoarthritis of TMJ (.OA group ) diagnosed by NMR and X-rayfluid samples (SF) and compared with clinical signs and symptoms as well as arthroscopic findings, examination and normal subjects useing by indirect aspiration technique. Secondly, the joint effusionThe levels of nitrites (NO2) 1>0.22 prnot/LI were measured by chemiluminescence assay andwadip dbyM anmtrxmalortnseMP)evleeasedyEISadisexpressd as N, and I- I 0>1.5 pgml) wa assaye by ELSA. MeaurwaseldiagnosedL byP Mer aandvmatrixee meterineprosenznagaeyManWest leveltweresia nsSedsmbyesLotaAnandy itsfound in F from paients, iit in SF fom healty asymptonatic conroactivitieswawereecdeterminedpibytoenzymograpySandaaWesterna:blot5analysisleinar SFisamples obtainedmo bythdirectTMJs with severe synovitis and/or cartilaginous degeneration. Furthermore, significantly higherasitoneciq(ShbaTera:19)Temlcursze fHAdfrdaogtetrelevels of NO were seen in the patients with TMD. In addition. the levels of NO were higher in painful groups. It was greatest for she normal group, followed by the ID group and the OA group, in that

The relationship between the acid and alkaline phosphatase activity and the adherence of Candida parapsilosis to human buccal epithelial cells

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The relationship between the acid and alkaline phosphatase activity and the adherence of Candida parapsilosis to human buccal epithelial cells

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