Ho, YW

Lau, WCS

Man, RYK

Hong Kong Medical Journal

HKU Scholars Hub

TitleEffects of Ganoderma lucidium (Lingzhi) on cell proliferation andcytokine production of synovial fibroblasts from rheumatoidarthritisAuthor(s) Ho, YW; Lau, WCS; Man, RYKCitationThe 8th Medical Research Conference Abstracts, Hong KongMedical Journal, Hong Kong, China, 25-26 January 2003, v. 9 n. 1Supp 1, p. 69Issued Date 2003URL http://hdl.handle.net/10722/46917Rights Creative Commons: Attribution 3.0 Hong Kong LicenseHKMJ Vol 9 No 1 February 2003 Supplement      69Effects of Ganoderma lucidium (Lingzhi) on cell proliferation and cytokine productionof synovial fibroblasts from rheumatoid arthritisYW Ho1, CS Lau2, RYK Man1, 1 Departments of Pharmacology and Medicine2, The University of Hong Kong, Hong Kong.Introduction: Ganoderma lucidium (Lingzhi), a medicinal mushroom, has been suggested to haveimmunomodulatory functions. The major chemicals with these effects appear to be polysaccharides and triterpenes.The purpose of this study was to investigate the effects of Lingzhi on RA synovial fibroblast proliferation andcytokine production including interleukin-1beta (IL-1beta), IL-6, IL-8, IL-10, tumor necrosis factor alpha (TNF-alpha) and monocyte chemotactic protein-1 (MCP-1).Method: Tissue samples were collected from patients with RA. The primary culture of synovial fibroblasts wasincubated with hot water extract of fresh Lingzhi polysaccharides for 24hr, 48hr 72hr and 96 hr at variousconcentrations. Proliferation of synovial fibroblasts was measured by thymidine incorporation and cytokine levelswere measured by enzyme-linked immunosorbent assay (ELISA).Results: Lingzhi had no effects on synovial fibroblast proliferation. RA synovial fibroblasts spontaneouslyproduced IL-6, IL-8 and MCP-1 but not IL-1 beta, IL-10 and TNF-alpha. Lingzhi had a slight dose and timedependent stimulatory effect on RA synovial fibroblast in IL-6 and MCP-1 production. Linghzhi also inhibited theproduction of IL-8 in a dose and time dependent manner but it exerted no effects on IL-1 beta, IL-10 and TNF-alphaproduction.Conclusion: Ganoderma lucidium has slight to moderate stimulating effects on un-stimulated RA synovial fibroblastin the production of IL-6 and MCP-1 and inhibitory effects on IL-8 production without stimulating synovial fibroblastproliferation. The effects of Lingzhi on cytokine synthesis by stimulated synovial fibroblasts, which mimic activedisease, are currently being studied.Use of health status measurement scales among arthritis patients with loweducational levelMY Chu1,BSc(OT), PKY Chiu2, RWS Wong1 and CS Lau11Department of Medicine, The University of Hong Kong, Hong Kong.1Department of Orthopaedics and Traumatology, The University of Hong Kong, Hong Kong.Purpose: Many researchers had advocated the use of self-administered health status measures such as ArthritisImpact Measurement Scales 2 for clinical evaluations in rheumatology. Frequently, illiteracy and patients’ age arefactors limiting the use of this form of assessment. The aim of this study was to evaluate the validity and reliabilityof using Chinese Arthritis Impact Measurement Scales 2 (CAIMS2) as a health assessment tool for patients withlow educational level.Methods: CAIMS2 was administered to 71 subjects [RA=14, OA=57] with arthritis using a standardizedinterviewing format. Reliability of scales, test-retest reliability and construct validity was evaluated and comparedto statistical results of a previous study on validity and reliability of CAIMS2 among literate clients.Results: Inter-item reliability of the CAIMS2 interviewed version [ICC: 0.6512 to 0.9825] was comparable to thatof the self-administered version [ICC: 0.8552 to 0.9594]. Test-retest reliability of the CAIMS2 interviewversion [r = 0.526 to 0.922] was, however, lower than the self-administered version [r = 0.77 to 0.95].Conclusion: Cautions have to be taken in administering CAIMS2 in an interviewed version. Further studies onhealth status measurement among illiterate patients is necessary to improve the feasibility of using such scales inclinical settings.NRI-09NRI-10

Effects of Ganoderma lucidium (Lingzhi) on cell proliferation and cytokine production of synovial fibroblasts from rheumatoid arthritis

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Effects of Ganoderma lucidium (Lingzhi) on cell proliferation and cytokine production of synovial fibroblasts from rheumatoid arthritis

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