Session - Respiratory & Critical Care Medicine: no.  G-RC-8published_or_final_versio

Chan, KN

Chu, LW

Ho, JCM

Hu, W

Lam, WK

Leung, R

Sun, JZ

Tsang, KWT

HKU Scholars Hub

Title The impact of aging on ciliary function and ultrastructive inhuman respiratory epitheliumAuthor(s) Ho, JCM; Lam, WK; Hu, W; Leung, R; Sun, JZ; Chan, KN; Chu,LW; Tsang, KWTCitation The 5th Medical Research Conference, Hong Kong, China,January 2000, v. 22 n. Supp 2, p. 20Issued Date 2000URL http://hdl.handle.net/10722/46805Rights Creative Commons: Attribution 3.0 Hong Kong LicenseG-RC-8THE IMPACT OF AGING ON CILIARY FUNCTION AND ULTRASTRUCTURE IN HUMANRESPIRATORY EPITHELIUMJames CM Ho. WK Lam, W Hu, R Leung, J Sun, KN Chan, LW Chu, KWT Tsang University Department ofMedicine, The University of Hong Kong, Hong Kong SARThe elderly have increased susceptibility to lower respiratory tract infections which is partly attributed toconcomitant respiratory diseases, gastro-oesophageal reflux, swallowing difficulties, and immobility. Althoughmucociliary clearance plays a crucial role in the defence against inhaled microbes, little is known on the effectof aging on respiratory ciliary function and ultrastructure. We have studied the ciliary beat frequency andultrastructure of respiratory cilia on 100 volunteers (53 males; mean ± S.D. age 52.1 ±24 yrs; range 11-90 yrs).Respiratory cilia were obtained from the inferior turbinate for measurement of ciliary beat frequency using anestablished photometric method. The mean ± S.D. ciliary beat frequency were 12.9+ 1.7 Hz (range 9.1-17).Transmission electron microscopy (TEM) examination was performed on ultrathin sections using a morphometricprotocol on all subjects. TEM examination revealed a significant increase in the frequency of microtubulardisarrangement and presence of single tubules with aging (p=0.002 and 0.005 respectively). The mean SDderived from the angles formed between the central microtubules of neighboring cilia was 12.4° (± 3.7°).Ciliary beat frequency negatively correlate with age (r=-0.45, p<0.001) but bore no gender difference (p>0.05).Our finding of impaired ciliary function and the increased frequency of ciliary ultrastructural anomalies withaging could help explain the frequent occurrence of respiratory infections in the elderly.G-RC-9CHEMORADIOTHERAPY IN ADVANCED LYMPHOEPITHELIOMA-LIKE CARCINOMA (LELC)OF LUNGJames CM Ho. WK Lam, +GC Ooi, *MP Wong, B Lam, KWT Tsang University Departments of Medicine,+Diagnostic Radiology, and 'Pathology, The University of Hong Kong, Queen Mary Hospital, Hong KongSAR, ChinaLymphoepithelioma-like carcinoma (LELC) of the lung, an Epstein-Barr virus (EBV)-associated undifferentiatedcarcinoma, is a rare entity of pulmonary malignancy. It appears to show predilection for young non-smokingAsians and is often resectable. However, little is known on the treatment of the even rarer locally advanced ormetastatic cases. We have conducted a prospective study on the use of combination chemotherapy (5-fluorouracil,leucovorin, and cisplatin) and radiotherapy in the treatment of locally advanced or metastatic LELC of lung.The inclusion criteria included age between 18 and 70 years, good performance state (WHO 0 or 1), histologicallyproven stage III or IV unresectable tumours, chemotherapy or radiotherapy naive, and nasopharyngeal carcinomaruled out by endoscopic biopsy and magnetic resonance imaging, Exclusion criteria included previousmalignancies, major organ failure and active uncontrolled infections. In-situ hybridization (ISH) for EBV-encoded small nuclear RNA (EBER) was performed on the biopsy specimens. Serological tests for EBV viralcapsid antigen were serially performed during chemotherapy. Chemotherapy constituted four 4-weekly cyclesof 5-fluorouracil (5-FU, 1000mg/m2/day on day 1 to 4), leucovorin (200mg/m2 on day 1 to 4), and cisplatin(100mg/m2 on day 1). Sequential local radiotherapy to mediastinum, given as 16x 2.5Gy/fraction, was includedfor locally advanced disease. Three cases (two females, mean age 42 years) of lung LELC were recruitedbetween July 98 to June 99. Serum IgA titres to EBV were raised to the upper limit of detection of ourlaboratory at >1/640 in all three cases. These remained at the same level throughout the course of chemotherapy.Two of the patients had adequate biopsy specimens for detection of EBER which were positive. The tumourresponse rate to chemotherapy was 67% (2/3) partial response and 33% (1/3) stable disease. The latter wasgiven local radiotherapy with partial response. Severe toxicities (NCI grade 3 or 4) included grade 3 vomiting(1 case) and grade 3 stomatitis (1 case). Our experience with the chemoradiotherapy helps in better understandingof the combined modality treatment in advanced or metastatic LELC of lung. This warrants future research inthe treatment of this rather chemoradiosensitive malignancy.- 20 -

The impact of aging on ciliary function and ultrastructive in human respiratory epithelium

http://hub.hku.hk/bitstream/10722/46805/1/51537.pdf

The impact of aging on ciliary function and ultrastructive in human respiratory epithelium

Abstract

Similar works

Full text

Available Versions

HKU Scholars Hub