Studio preliminare di utlizzo di seme criopreservato di Sparus aurata (L., 1758) in saggi ecotossicologici

Abstract

Feasibility to use cryopreserved Sparus aurata sperm, in ecotoxicological tests was evaluated. The reference toxicant was Cadmium, and as evaluation parameters considered were spermatozoa motility at activation and after 5 min. The EC50 level ranged from 41,8 to 1,0 ppm in relation to different incubation times (30min - 1h - 2h) and time from activation considere