Application of T-RFLP Analysis for Bacterial Community Structure of Colonies Grown on Agar Plates

Abstract

As a prompt and efficient method for analysis of community structure of bacterial colonies grown on agar plants, we investigated the application of terminal restriction fragment length polymorphism (T-RFLP) analysis. To deduce the community structure of colonies grown on agar olants, analysis of a number of colonies is necessary. In this analytical method, many colonies were collectively subjected to T-RFLP analysis to estimate the community structure of colonies. To analyze dominant bacterial soecies in test soils, 100 colonies were sufficient. Using this analytical method, we investigated the influence of planting method for colony count (pour-plate method vs. spread-plate method) on the constitution of bacterial species grown on the medium. Difference in plamting method did not markedly change the bacterial species grown, but it did affect the constitution ratio of some bacterial species