Using confocal microscopy, MEST-1-positive immunofluorescence was observed within various Trypanosoma cruzi forms, except in cell-derived trypomastigotes. Glycosylinositol phosphorylceramides were identified by thin-layer chromatography immunostaining as the antigens recognized by MEST-1 in these parasites. in epimastigotes, labeling of MEST-1 coincided with acidic vesicles, indicating an internal localization of these glycoconjugates.Universidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023900 São Paulo, BrazilWeb of Scienc

Mortara, Renato A.

Straus, Anita H.

Suzuki, Erika

Takahashi, Helio K.

Clinical and Diagnostic Laboratory Immunology

PubMed

Suzuki, Erika [UNIFESP]

Mortara, Renato A. [UNIFESP]

Takahashi, Helio K. [UNIFESP]

Straus, Anita H. [UNIFESP]

Repositório Institucional UNIFESP

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY,1071-412X/01/$04.000 DOI: 10.1128/CDLI.8.5.1031–1035.2001Sept. 2001, p. 1031–1035 Vol. 8, No. 5Copyright © 2001, American Society for Microbiology. All Rights Reserved.Reactivity of MEST-1 (Antigalactofuranose) with Trypanosomacruzi Glycosylinositol Phosphorylceramides (GIPCs):Immunolocalization of GIPCs in Acidic Vesiclesof EpimastigotesERIKA SUZUKI,1 RENATO A. MORTARA,2 HELIO K. TAKAHASHI,1 AND ANITA H. STRAUS1*Departamento de Bioquı´mica1 and Departamento de Microbiologia, Imunologia e Parasitologia,2Universidade Federal de Sa˜o Paulo, Escola Paulista de Medicina, Sa˜o Paulo, SP 04023-900, BrazilReceived 23 April 2001/Accepted 11 July 2001Using confocal microscopy, MEST-1-positive immunofluorescence was observed within various Trypanosomacruzi forms, except in cell-derived trypomastigotes. Glycosylinositol phosphorylceramides were identified bythin-layer chromatography immunostaining as the antigens recognized by MEST-1 in these parasites. Inepimastigotes, labeling of MEST-1 coincided with acidic vesicles, indicating an internal localization of theseglycoconjugates.Glycoconjugates containing galactofuranose (Gal-furanose)residues have been described in fungi, bacteria, and trypano-somatides such as Trypanosoma cruzi and Leishmania (4, 15–17). Although the biological role of Gal-furanose residues isstill unclear, the postulated absence of Gal-furanose and ga-lactofuranosidases in mammalian species suggests the intrigu-ing hypothesis that terminal Gal-furanose residues play a cen-tral role in survival of fungi and parasites by preventing theaction of the host’s glycosidases on their glycoconjugates. Ifthis hypothesis is correct, Gal-furanose residues are potentiallyuseful as specific target molecules for therapy of parasitic andfungal diseases.We recently characterized the mouse monoclonal antibody(MAb) MEST-1 (immunoglobulin G3 [IgG3]) (16), which rec-ognizes glycosylinositol phosphorylceramides (GIPCs) con-taining terminal residues of -D-Gal-furanose, in either thelinkage 1-6, present in the Pb-1 antigen of Paracoccidioidesbrasiliensis (6), or the linkage 1–3, present in GIPL-1 of Leish-mania major (8).In the present study, we analyzed the reactivity of MEST-1with different forms of T. cruzi. In the infected host, the intra-cellular dividing amastigotes give rise to trypomastigotes thatlyse the cell and spread the infection. Trypomastigotes may beingested by the triatomine vector and differentiate into divid-ing epimastigotes and then metacyclic trypomastigotes, theforms that are infectious to mammals. A lipopeptidophospho-glycan currently termed GIPC has been described as the majorglycoconjugate of the T. cruzi epimastigote surface (3). It is aGIPC with a well-known carbohydrate structure containingone or two terminal Gal-furanose residues (5, 10). We ana-lyzed the reactivity of MEST-1 with different forms of T. cruzi,and with GIPCs purified from the parasites, by indirect immu-nofluorescence (IFI), solid-phase radioimmunoassay (RIA),and high-performance thin-layer chromatography (HPTLC)immunostaining.Epimastigotes were grown in liver infusion tryptose mediumwith 10% fetal calf serum at 27°C (2). Trypomastigotes andextracellular amastigotes were isolated from culture superna-tants after 4 to 5 days infection of Vero cells with metacyclictrypomastigotes (1). Amastigote preparation contained lessthan 10% trypomastigotes, and trypomastigote preparationcontained less than 5% amastigotes. For IFI, formaldehyde-fixed parasites were incubated on coverslips treated with 0.1%polylysine (19). The adsorbed parasites were blocked with 5%bovine serum albumin (BSA) in phosphate-buffered saline(PBS) and incubated with MAb culture supernatant and 1%BSA containing 0.01 mM 4,6-diamidino-2-phenylindole(DAPI) and anti-mouse IgG conjugated to fluorescein isothio-cyanate. The coverslips were examined on a Bio-Rad 1024-UVconfocal microscope (1). Controls performed using an irrele-vant MAb (IgG3) showed no fluorescence. Strong fluorescencewas observed in epimastigotes (Fig. 1B) and extracellularamastigotes (Fig. 1F), and to a lesser extent in metacyclictrypomastigotes (Fig. 1D) and intracellular amastigotes (Fig.1J). Epimastigotes showed strong labeling of vesicle-like com-ponents in the posterior region of T. cruzi. In view of previousreports of acidic vesicles, termed reservosomes, in the poste-rior region of T. cruzi epimastigotes (9, 12), we analyzed therelationship between MEST-1 labeling and these vesicles. As afirst step, epimastigotes were incubated with Lysotracker Red,a specific label of acidic vesicles in eukaryotic cells (Handbookof Fluorscent Probes and Research Chemicals, MolecularProbes, Inc., Eugene, Oreg.), fixed with 1% formaldehyde, andincubated with MEST-1. Colocalization of Lysotracker Redand MEST-1 was confirmed (Fig. 2).In contrast to our results, a study by Golgher et al. (3) usinganti-GIPC serum showed a homogeneous label on the epimas-tigote surface, suggesting the predominant presence of GIPCs.We therefore characterized the T. cruzi antigen recognized byMEST-1 and analyzed the glycolipid fractions. Epimastigotes,culture-derived trypomastigotes, and extracellular amastigotes* Corresponding author. Mailing address: Departamento de Bio-quı´mica, Universidade Federal de Sa˜o Paulo, Escola Paulista de Me-dicina, Rua Botucatu 862, Sa˜o Paulo, SP 04023-900, Brazil. Phone andfax: 55-11-5579-2509. E-mail: straus.bioq@epm.br.1031FIG. 1. MEST-1 reactivity with various T. cruzi forms by confocal IFI. (A and B) Epimastigotes; (C and D) metacyclic trypomastigotes; (E andF) extracellular amastigotes; (G and H) Vero cells infected with trypomastigotes; (I and J) Vero cells infected with amastigotes. (A, C, E, G, andI) Differential interference contrast (bars show the scale in micrometers); (B, D, F, H, and J) fluorescence. Blue color, MEST-1 reactivity; red color,nucleus and kinetoplast stained with DAPI.1032 NOTES CLIN. DIAGN. LAB. IMMUNOL.FIG. 2. Colocalization of MEST-1 with acidic vesicles. Confocal immunofluorescence microscopy images of T. cruzi epimastigotes labeled withMEST-1 and Lysotracker Red are shown. (A) Phase contrast (bar, 5 m); (B) Lysotracker Red fluorescence; (C) MEST-1 fluorescence; (D)overlaid image of DAPI (blue), Lysotracker Red (red), and MEST-1 (green) fluorescence.VOL. 8, 2001 NOTES 1033were added to 96-well plates (precoated with 0.1% poly-L-lysine; 2  106 parasites in the first well), doubly diluted insubsequent wells, and fixed for 15 min with 0.5% glutaralde-hyde in cold PBS. MEST-1 reactivity was analyzed by solid-phase RIA (14). Fixed parasites were delipidated (or not) witha mixture of isopropanol-hexane-water (IHW) (55:20:25 [vol/vol/vol], upper phase discarded). Plates were then washed withPBS and used for solid-phase RIA (13). MEST-1 showed highreactivity with epimastigotes and extracellular amastigotes andweak reactivity with cell-derived trypomastigotes (Fig. 3A),similar to results from IFI. Delipidation of parasites abolishedMEST-1 reactivity, and, as expected, most of the antigenicitywas recovered in the organic extract (Fig. 3B) when the ex-tracts were adsorbed onto 96-well plates and MEST-1 bindingwas detected as described above.To confirm that MEST-1 recognizes all GIPC molecules andnot only a subpopulation present in acidic vesicles (whichcould explain the lack of epimastigote surface labeling withMEST-1), GIPCs were extracted from epimastigotes, extracel-lular amastigotes, and cell-derived trypomastigotes by homog-enizing the parasites (3  108) with 10 ml of isopropanol-hexane-water. The extracts were evaporated and dialyzed, andcarbohydrate content was determined after densitometry ofHPTLC stained with orcinol-H2SO4 (17). The GIPC fractionof epimastigotes contained 2 to 3 times more carbohydratethan that of amastigotes and 7.5 times more than that oftrypomastigotes (data not shown). Aliquots containing about 3g of carbohydrate of the various GIPC fractions were ana-lyzed by HPTLC on silica gel 60 plates (Whatman, Inc.,Clifton, N.J.), using as solvents chloroform-methanol-H2O at60:40:8 and 25:21:7 (vol/vol/vol). Upon staining with orcinol-H2SO4 (Fig. 4A), at least five glycolipid components with mi-gration expected for GIPCs were visualized for epimastigotes,and six components were visualized for cell-derived trypomas-tigotes and amastigotes. For HPTLC immunostaining, plateswere soaked in 0.5% polymethacrylate in hexane, dried,blocked for 2 h with 1% BSA in PBS, incubated overnight withMAb MEST-1, and incubated with rabbit anti-mouse IgG and125I-labeled protein A (2  107 cpm per 50 ml of BSA-PBS).GIPCs were reactive with MEST-1 (Fig. 4B), indicating thateven though GIPC levels vary among different T. cruzi forms(epimastigotes  amastigotes  trypomastigotes), all GIPCsexpress terminal Gal-furanose residues that are recognized byMEST-1. In order to better understand the role of GIPCs inthe T. cruzi life cycle, we are currently investigating GIPCmetabolic pathways. The relationship between GIPCs and epi-mastigote reservosomes is still unclear. Soares and DeSouza(11) showed by cytochemical studies that the main componentsof the reservosome matrix are proteins and lipid inclusions.The data presented here suggest that these inclusions containGIPCs, among other lipids.We thank Stephen Anderson for editing of the manuscript.This work was supported by FAPESP, CNPq, and PRONEX.FIG. 4. HPTLC of glycolipids from different forms of T. cruzi. (A)Staining with orcinol-H2SO4; (B) immunostaining with MAb MEST-1.Lane S, ganglioside standard mixture containing GM3, GM2, GM1,and GD1; lane 1, glycolipids from epimastigote forms; lane 2, amas-tigotes; lane 3, cell-derived trypomastigotes.FIG. 3. Reactivity of MAb MEST-1 with different T. cruzi forms. (A) Parasites (2 106) were serially diluted and adsorbed onto 96-well plates.Plates were treated with IHW (55:20:25, vol/vol/vol) (,E, and‚) or left untreated (,F, andŒ) and incubated with MEST-1. (B) After treatmentof parasites with IHW, the solvent was transferred to another 96-well plate and evaporated, and adsorbed glycolipid fractions were incubated withMEST-1.  and , epimastigotes; E and F, cell-derived trypomastigotes; ‚ and Œ, extracellular amastigotes.1034 NOTES CLIN. DIAGN. LAB. IMMUNOL.REFERENCES1. Barros, H. C., N. V. Verbisck, S. Da Silva, M. F. Araguth, and R. A. Mortara.1997. Distribution of epitopes of Trypanosoma cruzi amastigotes during theintracellular life cycle within mammalian cells. J. Euk. Microbiol. 44:332–344.2. Camargo, E. P. 1964. Growth and differentiation in Trypanosoma cruzi:origin of metacyclic trypomastigotes in liquid media. Rev. Inst. Med. Trop.Sao Paulo 6:93–100.3. Golgher, D. B., W. Colli, T. Souto-Padron, and B. Zingales. 1993. 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Amonoclonal antibody directed to terminal residue of -galactofuranose of aglycolipid antigen isolated from Paracoccidioides brasiliensis: cross-reactivitywith Leishmania major and Trypanosoma cruzi. Glycobiology 7:463–468.17. Toledo, M. S., E. Suzuki, A. H. Straus, and H. K. Takahashi. 1995. Glyco-lipids from Paracoccidioides brasiliensis. Isolation of a galactofuranose-con-taining glycolipid reactive with sera of patients with paracoccidioidomycosis.J. Med. Vet. Mycol. 33:247–251.18. Toledo, M. S., S. B. Levery, J. Glushka, A. H. Straus, and H. K. Takahashi.2001. Structure elucidation of sphingolipids from the mycopathogen Sporo-thrix schenkii: identification of novel glycosylinositol phosphorylceramideswith core Man1-6Ins linkage. Biochem. Biophys. Res. Commun. 280:9–24.19. Toledo, M. S., E. Suzuki, S. B. Levery, A. H. Straus, and H. K. Takahashi.2001. Characterization of monoclonal antibody MEST-2 specific to glucosyl-ceramide of fungi and plants. Glycobiology 11:105–112.VOL. 8, 2001 NOTES 1035

Reactivity of MEST-1 (antigalactofuranose) with Trypanosoma cruzi, glycosylinositol phosphorylceramides (GIPCs): Immunolocalization of GIPCs in acidic vesicles of epimastigotes

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Reactivity of MEST-1 (antigalactofuranose) with Trypanosoma cruzi, glycosylinositol phosphorylceramides (GIPCs): Immunolocalization of GIPCs in acidic vesicles of epimastigotes

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