An Hyperion hyperspectral image was used to investigate the spectral differentiation between several vegetation species. The region investigated was the Pirque zone, near Santiago, Chile, where vineyards can be identified in the image taken in 9 Feb. 2005. Fields with green and dried grass, and vineyards with several row orientations were selected, besides an area covered with sand. The shadow influence between rows in vineyards was investigated. Results show that the Hyperion image can effectively be used to differentiate between vegetation classes, and that shadow effects can influence the spectral response of soil with respect to the response of vegetation, in cases where there are mixture of information from these classes.Pages: 8524-852