Prémio de melhor poster científico.O uso dos serviços de urgência por utentes com problemas de saúde considerados não urgentes conduzem à sobrelotação destes serviços e consequente diminuição da satisfação do paciente e da qualidade dos serviços prestados. No entanto, a racionalização da utilização dos serviços de urgência depende da utilização adequada de outros serviços do sistema de saúde como os cuidados de saúde primários. Objetivo do estudo: Avaliar a importância dos Cuidados de Saúde Primários (CSP) na regulação dos serviços de urgência hospitalar.info:eu-repo/semantics/publishedVersio

Carvalho, Luciana R. de

Fiore, Marli F.

García, Carlos

Konno, Katsuhiro

Lagos Wilson, Néstor

Lorenzi, Adriana S.

Ríos, Fernanda R.

Sant'Anna, Célia L.

Silva-Stenico, María Estela

Rodrigues, Mariana Mira

Eiras, Margarida

RCIPL Repositório Científico do Instituto Politécnico de Lisboa

OS CUIDADOS DE SAÚDE PRIMÁRIOS COMO REGULADORES DO ACESSO ÀS URGÊNCIAS HOSPITALARESINTRODUÇÃOO uso dos serviços de urgência porutentes com problemas de saúdeconsiderados não urgentes conduzem àsobrelotação destes serviços e,consequente diminuição da satisfação dopaciente e da qualidade dos serviçosprestados. No entanto, a racionalizaçãoda utilização dos serviços de urgênciadepende da utilização adequada deoutros serviços do sistema de saúdecomo os cuidados de saúde primários.OBJETIVOAvaliar a importância dos Cuidados deSaúde Primários (CSP) na regulação dosserviços de urgência hospitalar.METODOLOGIAEstudo exploratório descritivo onde seutilizou um questionário para a recolhada informação, aplicado aos utentes compulseira verde do Serviço de Urgência doHospital de São José no dia 23 de Marçoe no dia 18 de Abril de 2016.Mariana Mira RodriguesDoutora Margarida Eiras, ESTeSL,CISPRESULTADOSCONCLUSÃOFigura 2 - Hospital de São JoséFonte: PAULO VAZ HENRIQUES (JornalExpresso)Na análise de resultados foi utilizadaestatística descritiva – percentagens efrequências, onde foi analisada umaamostra de 150 participantes.Figura 1 - Hospital de São JoséFonte: Jornal PúblicoAmostra45,3% com mais de 65 anos57,3% sexo feminino37,3% 1ºciclo do Ensino Básico44,7% ReformadosA maioria dos utentes releva que não tentou marcar consulta nocentro de saúde, apesar de terem médico de família atribuído. Defato, os inquiridos optam por escolher os serviços de urgência pelodesejo de receber cuidados no próprio dia e pela possibilidade derealizar qualquer tipo de exames de diagnóstico.Campanhas nacionais de sensibilização O questionário foi previamenteanalisado e validado por 2 peritos e,posteriormente submetido a um pré-teste. Frequência Percentagem  Não sei o que é a Linha de Saúde 24 60 44,8 A minha situação é urgente 38 28,3 Não me lembrei 26 19,4 Outra situação 10 7,5 Total 134 100,0   Frequência Percentagem  Atendimento no mesmo dia 45 36,9 Realização de exames de diagnóstico 28 23,0 Crença no serviço de urgência 11 9,0 Não tem médico de família 15 12,3 Outra 23 18,9 Total 122 81,3  É essencial reforçar os CSP para assegurar aos utentes o acesso a melhores cuidados de saúdeProlongamento de horas de serviço ou aumento da oferta de profissionais de saúdeEducação para uma utilização adequada dos serviços de saúde

Os cuidados de saúde primários como reguladores do acesso às urgências hospitalares

While evaluating several laboratory-cultured cyanobacteria strains for the presence of paralytic shellfish poison neurotoxins, the hydrophilic extract of Microcystis aeruginosa strain SPC777-isolated from Billings`s reservoir, So Paulo, Brazil-was found to exhibit lethal neurotoxic effect in mouse bioassay. The in vivo test showed symptoms that unambiguously were those produced by PSP. In order to identify the presence of neurotoxins, cells were lyophilized, and the extracts were analyzed by HPLC-FLD and HPLC-MS. HPLC-FLD analysis revealed four main Gonyautoxins: GTX4(47.6%), GTX2(29.5%), GTX1(21.9%), and GTX3(1.0%). HPLC-MS analysis, on other hand, confirmed both epimers, with positive Zwitterions M(+) 395.9 m/z for GTX3/GTX2 and M(+) 411 m/z for GTX4/GTX1 epimers. The hepatotoxins (Microcystins) were also evaluated by ELISA and HPLC-MS analyses. Positive immunoreaction was observed by ELISA assay. Alongside, the HPLC-MS analyses revealed the presence of [l-ser(7)] MCYST-RR. The N-methyltransferase (NMT) domain of the microcystin synthetase gene mcyA was chosen as the target sequence to detect the presence of the mcy gene cluster. PCR amplification of the NMT domain, using the genomic DNA of the SPC777 strain and the MSF/MSR primer set, resulted in the expected 1,369 bp product. The phylogenetic analyses grouped the NMT sequence with the NMT sequences of other known Microcystis with high bootstrap support. The taxonomical position of M. aeruginosa SPC777 was confirmed by a detailed morphological description and a phylogenetic analysis of 16S rRNA gene sequence. Therefore, co-production of PSP neurotoxins and microcystins by an isolated M. aeruginosa strain is hereby reported for the first time.FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)[2005/56303-5]FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)[2004/16042-5]CNPq (Conselho Nacional de Pesquisa)[308086/2004-0]CNPq (Conselho Nacional de Pesquisa)[140327/2004-5]FONDECYT (Fondo Nacional de Ciencia y Tecnologia-Chile)[1070706]FONDECYT (Fondo Nacional de Ciencia y Tecnologia-Chile)[1090058

SANT`ANNA, Celia L.

CARVALHO, Luciana R. de

FIORE, Marli F.

SILVA-STENICO, Maria Estela

LORENZI, Adriana S.

RIOS, Fernanda R.

KONNO, Katsuhiro

GARCIA, Carlos

LAGOS, Nestor

Universidade de São Paulo

Neurotoxicity Research

Highly Toxic Microcystis aeruginosa Strain, Isolated from So Paulo-Brazil, Produce Hepatotoxins and Paralytic Shellfish Poison Neurotoxins

Artículo de publicación ISIWhile evaluating several laboratory-cultured
cyanobacteria strains for the presence of paralytic shellfish
poison neurotoxins, the hydrophilic extract of Microcystis
aeruginosa strain SPC777—isolated from Billings’s reservoir,
Sa˜o Paulo, Brazil—was found to exhibit lethal
neurotoxic effect in mouse bioassay. The in vivo test
showed symptoms that unambiguously were those produced
by PSP. In order to identify the presence of neurotoxins,
cells were lyophilized, and the extracts were
analyzed by HPLC–FLD and HPLC–MS. HPLC–FLD
analysis revealed four main Gonyautoxins: GTX4(47.6%),
GTX2(29.5%), GTX1(21.9%), and GTX3(1.0%). HPLC–
MS analysis, on other hand, confirmed both epimers, with
positive Zwitterions M? 395.9 m/z for GTX3/GTX2 and
M? 411 m/z for GTX4/GTX1 epimers.
The hepatotoxins (Microcystins) were also evaluated by
ELISA and HPLC–MS analyses. Positive immunoreaction
was observed by ELISA assay. Alongside, the HPLC–MS
analyses revealed the presence of [L-ser7] MCYST-RR. The
N-methyltransferase (NMT) domain of the microcystin synthetase gene mcyA was chosen as the target sequence to
detect the presence of the mcy gene cluster. PCR amplification
of the NMT domain, using the genomic DNA of the
SPC777 strain and the MSF/MSR primer set, resulted in the
expected 1,369 bp product. The phylogenetic analyses
grouped the NMT sequence with the NMT sequences of
other known Microcystis with high bootstrap support. The
taxonomical position of M. aeruginosa SPC777 was confirmed
by a detailed morphological description and a phylogenetic
analysis of 16S rRNA gene sequence. Therefore,
co-production of PSP neurotoxins and microcystins by an
isolated M. aeruginosa strain is hereby reported for the first
time.This study was supported by grants from the
FAPESP (Fundac¸a˜o de Amparo a` Pesquisa do Estado de Sa˜o Paulo—
Grant N  2005/56303-5), from the CNPq (Conselho Nacional de
Pesquisa—Grant N  308086/2004-0), and from FONDECYT
N 1070706 and N 1090058 (Fondo Nacional de Ciencia y Tecnologia—
Chile). A.S. Lorenzi was supported by CNPq (Grant 140327/
2004-5) graduate scholarship, and M.E. Silva-Stenico was the recipient
of post-doctoral fellowship from FAPESP (Grant 2004/16042-5)

Repositorio Académico de la Universidad de Chile

Highly Toxic Microcystis aeruginosa Strain, Isolated from Sao Paulo—Brazil, Produce Hepatotoxins and Paralytic Shellfish Poison Neurotoxins

Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP)

Name not available

While evaluating several laboratory-cultured cyanobacteria strains for the presence of paralytic shellfish poison neurotoxins, the hydrophilic extract of Microcystis aeruginosa strain SPC777-isolated from Billings's reservoir, São Paulo, Brazil-was found to exhibit lethal neurotoxic effect in mouse bioassay. The in vivo test showed symptoms that unambiguously were those produced by PSP. In order to identify the presence of neurotoxins, cells were lyophilized, and the extracts were analyzed by HPLC-FLD and HPLC-MS. HPLC-FLD analysis revealed four main Gonyautoxins: GTX4(47.6%), GTX2(29.5%), GTX1(21.9%), and GTX3(1.0%). HPLC-MS analysis, on other hand, confirmed both epimers, with positive Zwitterions M + 395.9 m/z for GTX3/GTX2 and M + 411 m/z for GTX4/GTX1 epimers. The hepatotoxins (Microcystins) were also evaluated by ELISA and HPLC-MS analyses. Positive immunoreaction was observed by ELISA assay. Alongside, the HPLC-MS analyses revealed the presence of [l-ser 7] MCYST-RR. The N-meth

De Carvalho, Luciana R.

Silva-Stenico, Maria Estela

Lagos, Nestor

Highly toxic microcystis aeruginosa strain, isolated from São Paulo-Brazil,produce hepatotoxins and paralytic shellfish poison neurotoxinsSant'Anna, Célia L.De Carvalho, Luciana R.Fiore, Marli F.Silva-Stenico, Maria EstelaLorenzi, Adriana S.Rios, Fernanda R.Konno, KatsuhiroGarcia, CarlosLagos, NestorWhile evaluating several laboratory-cultured cyanobacteria strains for the presence of paralyticshellfish poison neurotoxins, the hydrophilic extract of Microcystis aeruginosa strainSPC777-isolated from Billings's reservoir, São Paulo, Brazil-was found to exhibit lethal neurotoxiceffect in mouse bioassay. The in vivo test showed symptoms that unambiguously were thoseproduced by PSP. In order to identify the presence of neurotoxins, cells were lyophilized, and theextracts were analyzed by HPLC-FLD and HPLC-MS. HPLC-FLD analysis revealed four mainGonyautoxins: GTX4(47.6%), GTX2(29.5%), GTX1(21.9%), and GTX3(1.0%). HPLC-MS analysis,on other hand, confirmed both epimers, with positive Zwitterions M + 395.9 m/z for GTX3/GTX2 andM + 411 m/z for GTX4/GTX1 epimers. The hepatotoxins (Microcystins) were also evaluated byELISA and HPLC-MS analyses. Positive immunoreaction was observed by ELISA assay. Alongside,the HPLC-MS analyses revealed the presence of [l-ser 7] MCYST-RR. The N-methy

Highly toxic microcystis aeruginosa strain, isolated from São Paulo-Brazil, produce hepatotoxins and paralytic shellfish poison neurotoxins

http://repositorio.ipl.pt/bitstream/10400.21/7203/1/Os%20cuidados%20de%20sa%c3%bade%20prim%c3%a1rios%20como%20reguladores%20do%20acesso%20%c3%a0s%20urg%c3%aancias%20hospitalares.pdf

Os cuidados de saúde primários como reguladores do acesso às urgências hospitalares

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RCIPL Repositório Científico do Instituto Politécnico de Lisboa

Universidade de São Paulo

Repositorio Académico de la Universidad de Chile

Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP)

Name not available

Repositorio Académico de la Universidad de Chile