Adult skeletal muscle possesses a resident stem cell population called satellite cells which are responsible
for tissue repair following damage. Satellite cell migration is crucial in promoting rapid tissue
regeneration but is a poorly understood process. Furthermore, the mechanisms facilitating satellite cell
movement have yet to be elucidated. Here the process of satellite cell migration has been investigated
revealing that they undergo two distinct phases of movement; firstly under the basal lamina and then
rapidly increasing their velocity when on the myofibre surface. Most significantly we show that satellite
cells move using a highly dynamic blebbing based mechanism and not via lamellopodia mediated
propulsion. We show that nitric oxide and non-canonical Wnt signalling pathways are necessary for
regulating the formation of blebs and the migration of satellite cells. In summary, we propose that the
formation of blebs and their necessity for satellite cell migration has significant implications in the future
development of therapeutic regimes aimed at promoting skeletal muscle regeneration
