School systems across the United States have been required by federal laws to utilize scientific based interventions and instruction within the classroom to educate all students. Through the use of a multi-tiered model called Response to Intervention (RTI), school systems now have a model to implement the interventions within the environment. The purpose of this study was to investigate secondary teachers’ knowledge of RTI within a public school system in the southeastern United States. It is vital that the teachers and specialists who implement RTI be knowledgeable of the multi-tiered model. The teacher’s knowledge of RTI can help guide administrators and professional development personnel as they plan for future trainings and implementation of new procedures