Development and optimization of high-throughput vertebrae screening platform

Abstract

Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2010.Cataloged from PDF version of thesis.Includes bibliographical references (p. 67-68).The high-throughput zebrafish screening platform is a revolutionary tool that enables subcellular precision in vivo whole animal screening of Danio Rerio. It can perform laser surgery and/or imaging in less than twenty seconds per fish. This is a two orders of magnitude improvement from the existing manual method, which takes 10 to 15 minutes per fish. The platform also has the ability to rotate the fish in any angle for imaging or laser surgery. There has never been a platform that could align and position a zebrafish consistently and efficiently in such manner before. This crucial functionality was realized through a design in which zebrafish was screened in a capillary tube.by Bryan Kyo Koo.M.Eng