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Preliminary characterization of ssps (Seed Storage Proteins) in Argania spinosa L.

Abstract

4 páginas, 2 figuras, 1 tabla.-- Comunicación presentada al 3rd International SMBBM Congress, IUBMB Special Meeting & 6th FASBMB Congress, celebrado en Marrakech (Marruecos) del 20 al 24 de Abril de 2009.SSPs (Seed Storage Proteins) of the 11S type have been preliminary characterized in the seeds of the argan tree, an endemic species from Morocco. Protein extracts from mature seeds were prepared by using different solutions in order to assess the solubility of the major protein forms. SSPs of the 11S type were classified as albumins according to the further SDS-PAGE analysis of these extracts. The combination of both reducing- and non-reducing conditions for the SDS-PAGE analysis, together with immunoblot experiments allowed us to determine the presence of three precursor forms of these proteins (pro1, pro2 and pro3), which are composed of six individual peptides (p1 to p6) in different combinations.This work was funded by Spanish BFU2004-00601/BFI and BFU2008-00629 projects. M. Allach thanks the research bursary granted by UNESCO/L´ORÉAL and L´ORÉAL Morocco.Peer reviewe

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