Structural rearrangements of chromosomes have played a decisive role in the karyotypic evolution of species. It is also known that inversions, translocations, fusions, fissions, heterochromatin variations and other chromosomal changes occur as transient events in natural populations. Herein we report the occurrence of a rare event of centric fission of a metacentric chromosome in a laboratory population of Drosophila, called Cytorace 1. This centric fission has been fixed in a sub-population of Cytorace 1, resulting in a new chromosomal lineage called Fissioncytorace-1