Decostere, A.

Schaeck, M.

Van Den Broeck, W.

Open Marine Archive

 - 82 -  
Beneficial effects of probionts in marine fish larvae and their modes 
of action  
Schaeck Marlien, Annemie Decostere and Wim Van Den Broeck 
Department of Morphology, Faculty of veterinary medicine, Ghent University, Salisburylaan 133, 
9820 Merelbeke, Belgium 
E-mail: Marlien.schaeck@hotmail.com  
The persistent use of antibiotics in aquaculture has resulted in antibiotic resistance, comprising 
environmental and human health risks. The use of beneficial bacteria or probionts might be an 
effective alternative in disease prevention and production enhancement. The use of probionts in 
aquaculture is popular as a numerous amount of scientific studies have reported improved survival 
and growth of fish larvae when supplied with probionts. However, there is a lack of knowledge on 
the modes of action of probionts and on their interaction with the aquatic host, which is of 
paramount importance to utilize the right probiotic strain to prevent specific diseases. As such, 
aquaculture production will be improved and the side-effects associated with the use of antibiotics 
reduced. The objective of this study is twofold.  
 
First, we selected and optimized probiotic isolates. The potential probiotics were cultured from 
healthy larvae and adult fish. The predictive screening tools used to assess probiotic potential were: 
1) the ability to adhere to and colonize the fish intestine; 2) the ability to suppress the growth of 
several important marine fish pathogens and 3) a lack of invasiveness or toxicity.  
 
Secondly, we will unravel the modes of action of the probionts, selected in the first objective, with a 
focus on disease resistance in the host organisms, applying innovative techniques e.g. gnotobiotic 
larval model systems, immune priming and laser capture microdissection. Sea bass (Dicentrarchus 
labrax), the major marine fish cultured in southern Europe, was employed as a model for marine 
fish. 
 


Beneficial effects of probionts in marine fish larvae and their modes of action

Schaeck, Marlien

Decostere, Annemie

Van Den Broeck, Wim

Ghent University Academic Bibliography

INTRODUCTION Infectious diseases in early life stages of fish constitute a limiting factor in the development of the aquaculture production. Up till recently, disease control has principally concentrated on the use of antibiotics, leading to antibiotic resistance. With the increasing demand for an environment-friendly aquaculture approach, the use of probiotics in aquaculture is now widely gaining acceptance. However,  there is a lack of knowledge  on the modes of action of the probiotics  and on their interaction with  the aquatic host, especially during the larval stages. RESULTS  300 isolates were acquired from  larval and adult Seas bass (Dicentrarchus labrax) and screened for probiotic potential:   easy culturable    Non-invasive or toxic   inhibition of the growth of pathogens   adhesion to intestinal  mucus    adhesion to intestinal  epithelium   FURTHER RESEARCH   In vivo research will be performed to determine   the  beneficial  effects of the selected probionts. The modes of action of the selected probionts will be investigated, with a focus on disease resistance. Sea bass larvae will be cultured under gnotobiotic conditions, an important tool to unravel the complex host–microbe interactions. The generated data will assist in developing strategies for better larval management through the manipulation of the intestinal microbial flora.   AIMS   select, develop and optimize probiotic isolates, based on both in vitro and in vivo observations.  unravel the modes of action of the probionts 

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https://biblio.ugent.be/publication/4096628/file/4096641

Beneficial effects of probionts in marine fish larvae and their modes of action

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