Bossier, P.

Defoirdt, T.

Vanmaele, S.

Open Marine Archive

Aquaculture and immunostimulation through the eyes of Artemia 
Vanmaele Sofie, Tom Defoirdt and Peter Bossier 
Laboratory of Aquaculture & Artemia Reference Center, Faculty of Bioscience Engineering, 
Department of Animal Production, Ghent University, Rozier 44, B-9000 Ghent, Belgium 
E-mail: sofie.vanmaele.sv@gmail.com  
Diseases still cause unpredictable and high mortalities in intensive crustacean culturing, leading to 
major economic losses. Controlling diseases has traditionally been done using antibiotics; however, 
resistance is now a major problem and they became a threat to human health and environment. To 
obtain a more sustainable production, new strategies for controlling pathogenic bacteria are 
necessary; for example immunostimulation and priming (the induction of resistance in the host 
organism using natural signaling molecules, i.e. immunostimulants). Immunostimulants, products 
derived from yeast or bacterial components, are widely used for aquaculture purposes. However, 
the efficacy of these compounds is not yet sufficiently validated, as are effects on the host organism 
and possible long-term effects (Smith et al., 2003). A simple model system using animals reared in 
a germ-free environment or with a known microorganism community (a gnotobiotic system), has 
been shown to be instrumental in studying immune effects. An excellent candidate for such a 
gnotobiotic model system is the crustacean Artemia franciscana, being a small and robust animal 
with a short life cycle. Larvae of Artemia are an important live-food in fish and shellfish larviculture 
and ingestion of pathogenic microbes may have dramatic consequences for both Artemia as 
animals that prey on them. For exploring the phenomenon of priming and immunostimulation a 
gnotobiotic system is being developed during this PhD project using axenic Artemia franciscana 
and a pathogen is being identified to perform challenge tests at different points in the life cycle. 
Vibrio campbellii LMG21363 is a known opportunistic pathogen for Artemia and is currently used as 
a standard strain for challenge tests using instar II nauplii of Artemia. Recently, there are newly 
identified pathogenic strains of Vibrio available of which our preliminary results showed at least one 
of them has a significantly higher virulence than the LMG21363 strain of Vibrio campbellii. Expected 
results from ongoing experiments will reveal more information about this strain, giving us a better 
understanding if it is better suited for challenge tests at different points in the life stage of Artemia 
compared to the standard Vibrio strain. It is anticipated that these experiments will also provide us 
information that will allow us to explore the immune response of gnotobiotic Artemia adults in 
future experiments, thus contributing to the discussion of immunostimulants for aquaculture 
purposes.  
 
References  
Smith V.J., J.H. Brown and C. Hauton. 2003. Immunostimulation in crustaceans: does it really protect 
against infection? Fish & Shellfish Immunology 15:71-90. 
 
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Aquaculture and immunostimulation through the eyes of <i>Artemia</i>

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