De Clerck, O.

Hollants, J.

Leliaert, F.

Leroux, O.

Willems, A.

Open Marine Archive

 - 38 -
THE ART NOUVEAU OF BIOLOGY: EXHIBITING SEAWEED-BACTERIAL 
SYMBIOSES 
Hollants Joke1,2, Olivier Leroux3, Frederik Leliaert2, Olivier De Clerck2 and Anne Willems1  
1 Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. 
Ledeganckstraat 35, 9000 Ghent, Belgium 
E-mail: Joke.Hollants@UGent.be   
2 Phycology Research Group, Department of Biology, Ghent University, Krijgslaan 281 (S8), 9000 
Ghent, Belgium 
3 Research Group Pteridology, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, 
9000 Ghent, Belgium 
 
Symbiosis highlights the beauty in biology. The symbiotic ‘two become one’ principle defines no 
longer a concept in biology; it basically is biology covering diverse forms of in nature widespread 
relationships among multiple partners and supporting fundamentally important processes. This 
essential significance of symbiosis is well outlined in the ‘endosymbiotic theory’ which claims that 
eukaryotic organelles like mitochondria and chloroplasts evolved by symbiogenesis. In the 
masterpiece of evolution algae and bacteria certainly take the leading part. As for their ancestors, 
marine macroalgae (seaweeds) form the modern-day canvas for heaps of bacterial symbioses 
ranging from beneficial, harmful or neutral, over obligate or facultative, to ecto- or endophytic 
interactions. Elaborating the latter, endobiotic associations between marine macroalgal hosts and 
bacteria have been reported over the past 40 years. Only in the siphonous (single celled, 
multinucleate) marine green alga Bryopsis, however, endogenous bacteria have been electron 
microscopically visualized in the cytoplasm at every stage of development, including the gametes, 
suggesting vertical transmission of the endosymbionts (Burr and West, 1970). This indicates a more 
stable and specific relationship between the algal host and its symbionts in which both partners 
may provide mutualistic ecological benefits. Although this remarkable algal-bacterial partnership 
was noticed in the early ‘70s, little or no research has been performed to explore the specificity and 
ecological significance of this alliance. To bring a new élan to this symbiosis-study, bacteria inside 
Bryopsis algae were visualized with fluorescent probes (FISH) and the endophytic bacterial diversity 
was analyzed by means of Denaturing Gradient Gel Electrophoresis (DGGE) and clone libraries. 
Sequencing results covered e.g. Rhizobiales, Rickettsiales, Rhodobacterales, and Bacteroides 
species with well-known symbiotic features. Moreover, when the identified endobacterial diversity 
was analyzed in regard to the Bryopsis samples’ phylogeny and geographic distribution, some 
patterns could be observed: Rhizobiales and Rhodobacterales species seem widespread in Bryopsis 
plants, whereas Rickettsiales and Bacteroides species are restricted to Bryopsis algae from, 
respectively, temperate and tropical seas. Also the phylogenies of the bacterial partners themselves 
indicate a more facultative life style of the Rhizobiales and Rhodobacterales species in contrast to a 
more obligate nature of the Bacteroides and Rickettsiales symbionts. These and additional results 
on the potential function and the host-, time- and site-specificity of the bacterial partners show 
Bryopsis algae harbor host-specific endobacterial communities which (1) are distinct from the 
epiphytic and surrounding seawater populations, (2) differ with geographic distribution of the algal 
samples, (3) have the potential to fix nitrogen in situ and (4) can vary over time indicating the 
presence of both facultative and obligate endosymbiotic bacteria. Future investigations will include 
quantitative PCR and FISH experiments with species-specific probes to fine-tune the bigger Bryopsis-
bacterial symbiosis picture.     
 
References  
Burr F.A. and J.A. West. 1970. Light and electron microscope observations on the vegetative and 
reproductive structures of Bryopsis hypnoides. Phycologia 9(1):17-37. 


The art nouveau of biology: exhibiting seaweed-bacterial symbioses

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