CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
Prokaryotic community structure in algal photosynthetic biofilms from extreme acidic streams in Rio Tinto (Huelva, Spain)
Authors
Angeles Aguilera
Linda A. Amaral-Zettler
+4 more
Ricardo Amils
Elena Gonzalez-Toril
Virginia Souza-Egipsy
Erik R. Zettler
Publication date
1 December 2008
Publisher
Spanish Society for Microbiology (SEM)
Doi
Cite
Abstract
Author Posting. © Spanish Society for Microbiology (SEM), 2008. This article is posted here by permission of Spanish Society for Microbiology (SEM) for personal use, not for redistribution. The definitive version was published in International Microbiology 11 (2008): 251-260, doi:10.2436/20.1501.01.69.Four algal photosynthetic biofilms were collected from the Rio Tinto (SW Spain) at four localities: AG, Euglena and Pinnularia biofilms; ANG, Chlorella and Pinnularia biofilms; RI, Cyanidium and Dunaliella biofilms; and CEM, Cyanidium, Euglena and Pinnularia biofilms. Community composition and structure were studied by a polyphasic approach consisting of 16S rRNA analysis, scanning electron microscopy by back-scattered electron detection mode (SEM-BSE), and fluorescence in-situ hybridization (FISH). Acidophilic prokaryotes associated with algal photosynthetic biofilms included sequences related to the Alpha-, Beta-, and Gammaproteobacteria (phylum Proteobacteria) and to the phyla Nitrospira, Actinobacteria, Acidobacteria and Firmicutes. Sequences from the Archaea domain were also identified. No more than seven distinct lineages were detected in any biofilm, except for those from RI, which contained fewer groups of Bacteria. Prokaryotic communities of the thinnest algal photosynthetic biofilms (<100 μm) were more related to those in the water column, including Leptospirillum populations. In general, thick biofilms (>200 μm) generate microniches that could facilitate the development of less-adapted microorganisms (coming from the surrounding environment) to extreme conditions, thus resulting in a more diverse prokaryotic biofilm.V.SE. and A.A are supported by a Ramón y Cajal contract from the Ministerio de Educación y Ciencia. EG-T. is supported by Instituto Nacional de Técnica Aeroespacial (INTA). This work was supported by a grant from the MECSpain (CGL2005-05470/BOS) and by Centro de Astrobiología (CSICINTA) and a grant from NASA Astrobiology Institute (NC-1054 LAZ)
Similar works
Full text
Open in the Core reader
Download PDF
Available Versions
Digital.CSIC
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:digital.csic.es:10261/1302...
Last time updated on 25/05/2016
Woods Hole Open Access Server
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:darchive.mblwhoilibrary.or...
Last time updated on 08/06/2012