CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
unknown
Extracellular signal-regulated kinase 1/2 activity is not required in mammalian cells during late G2 for timely entry into or exit from mitosis
Authors
Abbott D. W.
Aguirre Ghiso J. A.
+73 more
Aguirre Ghiso J. A.
Alexei V. Mikhailov
Alvarez B.
Bartkova J.
Chung E.
Conly L. Rieder
Crosio C.
Dangi S.
Davies H.
Davies S. P.
Edelmann H. M.
English J. M.
Ferrell J. E.
Giroux S.
Gomez-Cambronero J.
Gonzalez F. A.
Gotoh Y.
Grill C.
Guadagno T. M.
Hahn W. C.
Harding A.
Hayne C.
Hayne C.
Hendzel M. J.
Horne M. M.
Julio A. Aguirre-Ghiso
Khodjakov A.
Kiyokawa N.
Klein S.
Knauf J. A.
Laird A. D.
Laoukili J.
Liao H.
Liu X.
Lou Y.
Ma R. Y.
Mainiero F.
Mikhailov A.
Mikhailov A.
Minshull J.
Mio Shinohara
Mody N.
Newberry E. P.
Orna Cohen-Fix
Pages G.
Pearson G.
Pines J.
Reszka A. A.
Rieder C. L.
Roberts E. C.
Roovers K.
Saavedra H. I.
Schmid-Alliana A.
Sebolt-Leopold J. S.
Seger R.
Shapiro P. S.
Shapiro P. S.
Shaul Y. D.
Sif S.
Squires M. S.
Takenaka K.
Takenaka K.
Tamemoto H.
Tang D.
Uchiyama S.
Wang X. M.
Widmann C.
Willard F. S.
Wojnowski L.
Wright J. H.
Yan Y.
Yoon S.
Zecevic M.
Publication date
11 October 2006
Publisher
'American Society for Cell Biology (ASCB)'
Doi
Cite
View
on
PubMed
Abstract
Author Posting. © American Society for Cell Biology, 2006. This article is posted here by permission of American Society for Cell Biology for personal use, not for redistribution. The definitive version was published in Molecular Biology of the Cell 17 (2006): 5227-5240, doi:10.1091/mbc.E06-04-0284.Extracellular signal-regulated kinase (ERK)1/2 activity is reported to be required in mammalian cells for timely entry into and exit from mitosis (i.e., the G2-mitosis [G2/M] and metaphase-anaphase [M/A] transitions). However, it is unclear whether this involvement reflects a direct requirement for ERK1/2 activity during these transitions or for activating gene transcription programs at earlier stages of the cell cycle. To examine these possibilities, we followed live cells in which ERK1/2 activity was inhibited through late G2 and mitosis. We find that acute inhibition of ERK1/2 during late G2 and through mitosis does not affect the timing of the G2/M or M/A transitions in normal or transformed human cells, nor does it impede spindle assembly, inactivate the p38 stress-activated checkpoint during late G2 or the spindle assembly checkpoint during mitosis. Using CENP-F as a marker for progress through G2, we also show that sustained inhibition of ERK1/2 transiently delays the cell cycle in early/mid-G2 via a p53-dependent mechanism. Together, our data reveal that ERK1/2 activity is required in early G2 for a timely entry into mitosis but that it does not directly regulate cell cycle progression from late G2 through mitosis in normal or transformed mammalian cells.This research was supported by National Institutes of Health Grant GMS-40198 to C.L.R., by National Institutes of Health/National Cancer Institute Grant CA109182, and Samuel Waxman Cancer Research Foundation grants to J.A.A.-G
Similar works
Full text
Open in the Core reader
Download PDF
Available Versions
Woods Hole Open Access Server
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:darchive.mblwhoilibrary.or...
Last time updated on 08/06/2012
Crossref
See this paper in CORE
Go to the repository landing page
Download from data provider
Last time updated on 01/04/2019