CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
unknown
Ena/VASP proteins have an anti-capping independent function in filopodia formation
Authors
Adams J. C.
Ahern-Djamali S. M.
+75 more
Albrecht-Buehler G.
Bachmann C.
Ballestrem C.
Bartles J. R.
Barzik M.
Bear J. E.
Bear J. E.
Bearer E. L.
Bohil A. B.
Carl U. D.
Chakraborty T.
Chereau D.
Co C.
David Drubin
Dent E. W.
Derek A. Applewhite
Dubin-Thaler B. J.
Ermekova K. S.
Evangelista M.
Fedorov A. A.
Frank B. Gertler
Gary G. Borisy
Gertler F. B.
Gertler F. B.
Han Y. H.
Harbeck B.
Hashimoto Y.
Haviv L.
Horstrup K.
Howe A. K.
Huttelmaier S.
Jacinto A.
Jacinto A.
Kojima S.
Kovar D. R.
Krause M.
Krause M.
Kuhnel K.
Kwiatkowski A. V.
Lambrechts A.
Lanier L. M.
Laurent V.
Lebrand C.
Loitto V. M.
Loomis P. A.
Mejillano M. R.
Melanie Barzik
Mitchison T. J.
Mogilner A.
Mogilner A.
Niebuhr K.
Pellegrin S.
Pollard T. D.
Pollard T. D.
Porter K. R.
Reinhard M.
Rottner K.
Samarin S.
Schafer D. A.
Schirenbeck A.
Schirenbeck A.
Shin-ichiro Kojima
Small J. V.
Svitkina T. M.
Svitkina T. M.
Tatyana M. Svitkina
Tilney L. G.
Vasioukhin V.
Vignjevic D.
Vignjevic D.
Walders-Harbeck B.
Waldmann R.
Zigmond S. H.
Zigmond S. H.
Zimmermann J.
Publication date
2 May 2007
Publisher
'American Society for Cell Biology (ASCB)'
Doi
Cite
View
on
PubMed
Abstract
Author Posting. © American Society for Cell Biology, 2007. This article is posted here by permission of American Society for Cell Biology for personal use, not for redistribution. The definitive version was published in Molecular Biology of the Cell 18 (2007): 2579-2591, doi:10.1091/mbc.E06-11-0990.Filopodia have been implicated in a number of diverse cellular processes including growth-cone path finding, wound healing, and metastasis. The Ena/VASP family of proteins has emerged as key to filopodia formation but the exact mechanism for how they function has yet to be fully elucidated. Using cell spreading as a model system in combination with small interfering RNA depletion of Capping Protein, we determined that Ena/VASP proteins have a role beyond anticapping activity in filopodia formation. Analysis of mutant Ena/VASP proteins demonstrated that the entire EVH2 domain was the minimal domain required for filopodia formation. Fluorescent recovery after photobleaching data indicate that Ena/VASP proteins rapidly exchange at the leading edge of lamellipodia, whereas virtually no exchange occurred at filopodial tips. Mutation of the G-actin–binding motif (GAB) partially compromised stabilization of Ena/VASP at filopodia tips. These observations led us to propose a model where the EVH2 domain of Ena/VASP induces and maintains clustering of the barbed ends of actin filaments, which putatively corresponds to a transition from lamellipodial to filopodial localization. Furthermore, the EVH1 domain, together with the GAB motif in the EVH2 domain, helps to maintain Ena/VASP at the growing barbed ends.This work was supported in part by National Institutes of Health Grants GM7542201 to D.A.A., GM58801 to F.B.G., and GM62431 to G.G.B. and by Cell Migration Consortium Grants GM64346 to D.A.A and G.G.B
Similar works
Full text
Open in the Core reader
Download PDF
Available Versions
Crossref
See this paper in CORE
Go to the repository landing page
Download from data provider
Last time updated on 01/04/2019
Woods Hole Open Access Server
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:darchive.mblwhoilibrary.or...
Last time updated on 08/06/2012