This study aims to examine psychometric properties of Moore version of World Health Organization's Quality of Life assessment short instrument in HIV patients (WHOQOL HIV-BREF). A study was conducted on 100 persons living with HIV/AIDS in Ouagadougou, Burkina Faso. The internal consistency was evaluated using Cronbach's α. For the convergent validity of the Moore version WHOQOL-HIV BREF, the Satisfaction With Life Scale (SWLS) was used as a comparison instrument and cross-product correlations were calculated. The test-retest reliability was assessed using the interclass correlation coefficient. The study showed high internal consistency with Cronbach's α at 0.92 for the whole instrument. According to domains, Cronbach's α found was ranged from 0.58 to 0.87, showing acceptable internal consistency for all domains. Domains scores for test-retest reliability, using Interclass Correlation gave coefficients ranged from 0.40 (Spiritual domain) to 0.99 (Level of Independence domain) with p0.05). As a demonstrated cross-cultural instrument, the WHOQOL HIV-BREF in its Moore version can be used for quality of life assessment in a routine way or longitudinal studies in Burkina Faso with persons living with HIV/AID