Novel ligature methods for studying sublethal effects of sit-and-wait predators: test using Cordulegaster boltonii (Donovan, 1807) larvae (Anisoptera: Cordulegasteridae)

Abstract

A novel method of labial palp ligature was tested as a substitute for palp ablation for studying sublethal effects of larvae of C. boltonii on prey populations and their consequences for ecosystem functioning. Two alternative types of ligature were designed to test for neutral or aggressive, but non-lethal, predator-prey interaction effects. Ligature efficiency in preventing prey capture was very high and the effects on larval survival and emergence success were negligible. Potential advantages and drawbacks, compared to other methods, are discussed. The results indicate that this fully reversible method should be applied whenever possible, especially for naturally rare or endangered odon. spp