Nikbakhtzadeh, Marjan

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,.5(,i; gi.3lX*.r,,i.rf .#Li.lls'C'h* lnU Olr-b":ollEO[J/U jl ,.Eest: PTSD Gtgf J*o ;c cs.sf rlny , cb orc t e.rgoclj e.aq e& Ot+y : Jr*l.dq.i Apg ifc t Loill; ch.rl(.5J€**, tAy)t ;,-o : .)9tia ch.rtlflt :o{aaO JL,eResponse to acute and chronic facial pain in rat model ofPTSD : Study the effect of naloxoneA thesisPresented toTire Graduate StudiesBy)Iarjan \ikbakht ZadehIn par-tial fulfillmentof requii'ernents for the degreeMaster of Science in:Human PhysiologyKerman University of medical sciencesJun 2015:o-tl.}cr. 0l)65 ),,) :,V(,; U (POst traumatic stress disorder ) PTSD ,"J)t' of jia) s' Jt;i ,r<'Jt oUlt' :''i'uLAJIt 11 ..is.:,,lj5 r"9l|;i t;U-1 9':lrrL-r1 6l;v3 r;tUil 1) 6'e9Gl";*o)'*;s1y L"o"f'-'")I-l 'u:,!.:r.: p r.: ll,-5,ru ;\ 4)iy-1 1; o9)t-,;-l PTSD .lli*' Jr" .t) rtvo c)"r-, )E ))) 4 !*i -^) &t' u\'r: "-'N. 4f ,lf Lru)N ))9, P iYf g )Vrb.,1 ;l vr.r;i o:Uil ,"f \"-YA' i rL-'-9 ':l; u'),2- "if :ro c-'r'o 9,'r I r- ) fV all't' 1r:l-a t'1l'91 9119"J-.r, s "e)Q ;L slst,s\.)),-iss 4. SPS rl"r r--U e (Single Prolong Stress) SPS ;" o, PTSDEye .srli, ,Jl^ orjl L,i c,t-:r: , SPS .t.F r (Ctl) JrS 'gf u'.1s2" dG.r"r ''''b\ er" o'-#$y. a-r..i.li; $lr (r mg/kg) ,u.tdu ur-ri ! "l)jq .,uf ),t c.du-j)l))r;Jtl ,r;1 e,sf oee..,9"J 'wiping,-fu ))r,"J /i o9f 9t ;))) dJ &-,.:.r*,L^" ;19 ;-1; o- 9 YL !.r).srb l-j Jfu o' .*Jt'p;y;1 'l 'fp a;iill.,*f )pd,.,u.^ 9,c.sl,- .511r *j-.-,blt Ctl ,r; e + SPS .rf 1) o9)t-;I.;lq;li! 1) o$ "52 o ,tG) g sltg r[; :6y-hi;1-ol-(.511: ,:* t*q Ctl+Dexa o925 ! a--u;,: SPS*Dexa ogss 2: ';9;['1fr '-ytv )\ t* '-'si-#$ olt6p a99.-EG uL)lir Jt'l il ,l 6-[ zr:Ll-1 ,;]t- ,;"o15 Ctl ! a--U" 1l SPS ol,5 csla ..r"r,r) rt;? 'lL ll-r': 'c"iL-:r:.,1 ,r,y-l ,SPS "9f ,:.c^sL- Ctl "9f .Jt6 d,f ! *-1" i ,1tb G* r,i""lPl 'SPS ogf 's'\F- dG dr otf tto.t i,, J; \l:..- JL., L.-lp r: ,iLJ,rU &-r.p .r r .jlK f., u;r- \OJtl .r-,i,'1 ,r;L;'"i 9 rL :': o*;U 'o$11r ;,* ..,r-1.p1 '.- r1*5ru J,)i ,SPS ;l *;U ,,;-l 4.lt- i a(JE 2r l; Ctl oe)t ! n'--UL P cs"f e rE lls 2r rlr.r,-,:rf Ctl !.rp ! a*,"u" ;) uzee );)9rLrl:Jlrll 9[i-ry'1 .,;L.J Sr>,na;c*;c-Lo'SPSr*iU.-'"1-] t'-l/,:a(:1:;L::';alit"e'-1 :6f tao;.ri I rL rrr Jlr:l .,) .,,,F1 b-lf .,) "Jl"' il , JL,; t'-1p )) ci))) -r"; ol,jl d)u;;'U o9)Q '+U- t'" 'Jblt ot' c>'y1r;j9J1 .sGrJXl YL:>l at *,SF a''i in+ r';E aJIt" r'Llr' 'c-l a^il:;!t'i 'tv")\ 'i a'\g- AG Js" otv'.rllr pq oJlt + )V ^< ,5 *. '1^' Jti t -l).j { 4 ;rur' Jtu + PTSD oule-G.\*- d;r e SPS ,JLJrU,PTSD,.y4 )tr:1::.5"r*l'l oUAbstractIntroduction: Clinical studies indicate that the synptoms of post-traumatic stress disorder(PTSD) are associated with pain problem. Previously study reporled that chronic stress caninduce horrnonal and neural changes which can affect the pain behavior and threshold. Thepurpose of this study was to evaluate the response to acute and chronic facialpain and the effectof naloxone on this pain in animal model of PTSD.Method and matherials: One hundred and thirty seven male wistar rat were used in this study.After PTSD induced by single prolong stress (SPS) method and confirmed by Elevated PlusMaze (EPN{) and plasma cofiicostrone assa}'in control (Ctl) and SPS groups. Facial pain wereevaluated by behavioral assessment such as. eye wiping test, von frey test and fonnalin test.Furthurmore, nociceptive sensitivitl,' ur these two groups was measured by injection of naloxone(3mg/kg ,i.p), 30 minutes before fon-nalu-r irlection (50 macroliter, ll59/o,s.c) in right side of theupper lip.Result: Frequency of entries and tlme spent in open arms of elevated plus maze weresignificantly decreased ur SPS group compared to Ctl Ard also, cofiicostrone levels alterdexamethasone injection decreased rn SPS-Dexa group compared to Ctl+Dexa. Nurnber of eyewiping decreased in SPS group compared to Ct1. The response threshold of applying von freyfilaments increased in SPS group colrpa:eC ro Ct1. In SPS group, first and second phase of paindecreased after formalin injection. In non-na, condition, naloxone injection were decreased thefirst and secondphase of paincompared to Ctl, while naloxone injectionin stress conditioq wereincreased tlie facial pain compared to Ct1.Coclusion: The result showed that the sensitivity to chemrcal irritant, allodynia and theperception of acute and chronic facial pain uere decreased, in SPS stress condition. Furthunnore,naloxone 1-iad an anti-nociceptive effect on acute and chronic facial pain perception in normalcondition but had an opposite effect rn stress condition. According to this study, it rvas concludedthat endogenous opioids may probably acts iiifferently in PTSD animals compared to normal, thatneeds fu rther investigation.LIc,d#fldtKeywords: Acute and chronic paint PTSDI Naloxonel SPSI rat

Response to acute & chronic facial pain in rat model of PTSD : Study the effect of naloxone

http://eprints.kmu.ac.ir/21982/1/5126.pdf

Response to acute & chronic facial pain in rat model of PTSD : Study the effect of naloxone

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