PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN PEGAGAN (Centella asiatica L. Urban) TERHADAP KERUSAKAN STRUKTUR HISTOLOGIS SEL HEPAR MENCIT (Mus musculus) YANG DIINDUKSI PARASETAMOL DOSIS TOKSIK

Abstract

Naila Shofwati Putri, G0011146, 2014. The Effect of Ethanolic Extract of Pegagan Leaves (Centella asiatica L. Urban) on Histological Damage of Hepatocyte on Paracetamol Induced Mice Model. Mini Thesis, Faculty of Medicine, Sebelas Maret University, Surakarta. Background: Pegagan Leaves (Centella asiatica L. Urban) contain antioxidative compounds, that can protect hepatocytes from free radicals. The aim of this research is to know the effect of ethanolic extract of Pegagan Leaves (Centella asiatica L. Urban) on histological damage of hepatocyte on Paracetamol induced mice model. Methods: This study was an experimental laboratory with the post test only control group design. Samples were 30 male Swiss webster mice, with 2-3 months old and ± 20 gr body weight, divided into 5 groups equally, namely Kelompok Normal (KN), Kelompok Induksi (KI), Kelompok Perlakuan 1 (KP1), Kelompok Perlakuan 2 (KP2), and Kelompok Perlakuan 3 (KP3). KN and KI were given aquadest for 14 days. KP1, KP2, and KP3 were given ethanolic extract of Pegagan Leaves in dose 5 mg/20 gbw, 10 mg/20 gbw, and 20 mg/20 gbw respectively for 14 days. Paracetamol in dose 5.07 mg/20 gbw were given to KI, KP1, KP2, and KP3 on the 12th, 13th, 14th day. On the 15th day, mice were sacrificed and livers were taken for preparation with Paraffin Block method and stained HE. The features of histological damage of hepatocyte were measured by counting the numbers of nucleus damage of hepotcyte (pyknosis, karyorrhexis, karyolysis) of 100 cells in the sentrolobuler zone. The data was analyzed by One-Way ANOVA test and continued by Post Hoc LSD test. Results: The means of histological damage of hepatocyte were 16.08 ± 5.160; 66.00 ± 3.275; 44.58 ± 5.583; 31.67 ± 2.188; 26.17 ± 3.810 for KN, KI, KP1, KP2, KP3 respectively. The result of One-Way ANOVA test showed a significant difference of mean, at least in two groups, *p = 0.000. The result of Post Hoc LSD test also showed a significant difference between five groups, *p = 0.000 for KN-KI, KN-KP1, KN-KP2, KN-KP3, KI-KP1, KI-KP2, KI-KP3, KP1-KP2, KP1-KP3 and *p = 0.002 for KP2-KP3 Conclusion: The ethanolic extract of Pegagan Leaves can prevent histological damage of hepatocyte on Paracetamol induced mice model. Keywords: Ethanolic extract of Pegagan Leaves, Paracetamol, histological damage of hepatocyt