Institute for Medical Research and Occupational Health
Abstract
Ispitan je efekt 1,2-dihlorbenzena, 1,2,3,4- tetrahlorbenzena, 1,2,3,5- -tetrahlorbenzena, pentahlorbenzena, heksahlorbenzena i Arohlora 1254 na aktivnost enzima lipoperoksidaze i ksantinoksidaze, sadržaj citohroma P-450 i glutationa u izolovanim hepatocitima. Vijabilnost hepatocita bila je smanjena kod primene svih ispitivanih supstancija. 1,2- dihlorbenzen je smanjio sadržaj citohroma P-450 kao i aktivnost enzima, a ostali hlorbenzeni povećavali su aktivnosti enzima, ali nisu uticali na sadržaj citohroma P-450. Arohlor 1254 je povećao sadržaj citohroma P-450, a nije uticao na enzimsku aktivnost. Svi ispitivani spojevi su smanjili sadržaj glutationa.The effects of 1,2-dichlorbenzene, 1,2,3,4-tetrachlorbenzene, 1,2,3,5-tetrachlorbenzene, pentachlorbenzene, hexachlorbenzene and Arochlor 1254 on hepatocyte functions were investigated. The viability of hepatocytes as well as glutathione concentration were decreased in all treatments. 1,2- dichlorbenzene diminished the content of cytochrome P-450 and decreased lipoperoxydase and xantinoxydase activities. Under the same conditions the other compounds investigated increased lipoperoxydase and xantinoxydase activities without influencing cytochrome P-450 content. Arochlor 1254 increased the content of cytocrome P-450 in hepatocytes, but had no effect on enzyme activities
