Tremorogeni učinci ekstrakata plijesni izoliranih iz prašine drveta u štakora

Abstract

Among workers in the Swedish wood industry acute alveolitis, known as “woodtrimmer\u27s disease” has been recognized for some time. One of the signs in the acute phase of the disease is transitory tremor. The etiology of the disease is still not clearly understood, but it is assumed to be associated with wood dust containing fungal spores. One of the dominant fungal strains isolated from wood at the saw-mills was found to be Aspergillus fumigatus. As it is known that Aspergillus fumigatus has the ability to produce tremorgenic mycotoxins, the moulds from the working environment were isolated, cultivated and tested for the ability to produce mycotoxins, to estimate a possible relation between fungal spores and tremorgenic effects. Extracts of moulds were prepared and tested on rats after oral administration. The ability to induce tremors in rats was found in six out of nine extracts tested. A very good agreement was found between the results of the chemical analysis of extracts and those of the study in vivo. The results imply that woodtrimmer\u27s disease may at least partly, be mycotoxicosis.U Švedskoj, u radnika zaposlenih u drvnoj industriji, javlja se akutni alveolitis poznat i pod nazivom Woodtrimmer\u27s disease, popraćen prolaznim tremorom. Pojava je nepoznate etiologije ali se povezuje s izloženošću drvenoj prašini u kojoj se nalaze spore različitih plijesni. Aspergillus fumigatus je plijesan koja se najčešće izolira u sušarama drveta. Iz radne sredine izolirane su plijesni Aspergillus flavusa te je nakon kultivacije testirana njihova sposobnost stvaranja tremorogenih mikotoksina analitičkim metodama i u pokusima na štakorima. Ekstrakti plijesni aplicirani su pokusnim životinjama oralno. Šest od devet ekstrakata izazvalo je pojavu tremora u tretiranih životinja. Nađena je vrlo dobra korelacija rezultata kemijskih analiza i rezultata dobivenih u pokusima in vivo. Podaci upućuju na moguću vezu između Woodtrimmer\u27s disease i izloženosti radnika tremorogenim mikotoksinima