Najveći problem vezan uz područje mikrobiologije goriva jest što mikrobiološke infekcije uzrokuju gubitak kvalitete proizvoda, formiranje muljeva, naslage na filtrima, cijevima i ventilima, oštećenja cjevovoda i spremnika goriva u rafinerijama zbog procesa biokorozije i time visoke troškove popravaka ili zamjene dijelova opreme, promjene u radu motora i, na kraju, gubitak ugleda (korisnici govore o problemima). U radu su prikazani rezultati ispitivanja potencijala autohtone mikrobne populacije izolirane iz tla za biorazgradnju dizelskog goriva (eurodizel) i biodizela (metilnih estera masnih kiselina FAME). Testirane su mješavine dizelskog goriva s 10 % (v/v) FAME (B10), dizelsko gorivo i biodizel (B100). Također su iz dizelskog goriva i biodizela izolirane mješovite kulture mikroorganizama. Rezultati istraživanja pokazali su da mješovita mikrobna populacija koja nije prethodno bila izložena naftnim ugljikovodicima u laboratorijskim uvjetima razgrađuje FAME u potpunosti nakon šest dana, a čisto dizelsko gorivo također, ali u manjoj mjeri. Razlika je uočena i u brzini rasta ovisno o izvoru ugljika (FAME ili čisti eurodizel) kao i u uvjetima rasta ovisno o sastavu hranjive podloge za uzgoj. Iz rezultata se može zaključiti da će u cijelom lancu proizvodnje, skladištenja i opskrbe uz dodatak FAME biti pojačana mikrobna proliferacija što može, ukoliko se ne primjenjuju mjere održavanja čistoće u svim segmentima proizvodnje i distribucije, imati posljedicu smanjenja kvalitete goriva, pojavu biokorozije i, na kraju, pad ugleda distributera na tržištu. Cilj je bio upoznati upravu kompanije s kojom vrstom problema vezanih na kvalitetu goriva će se suočiti uvođenjem biodizela u proces proizvodnje kao i na vrijeme uvesti brze i točne metode za utvrđivanje mogućih mikrobnih infekcija. Također, na temelju rezultata istraživanja, stanja lanca opskrbe i distribucije te iskustava naftne industrije u svijetu, predložene su preventivne i zaštitne mjere.The mayor microbial problem in the petroleum refining industry in the world is that microbial contamination of stored products leads to loss of product quality, sludge formation, deterioration of pipework and storage tank in the refinery due to bio corrosion processes, huge costs of equipment reparation and replacement, deposition and blockage of filters, pipes, valves, engine failures and finally loss of reputation (customers are talking about).
In this paper the results of biodegradability potential of autochthonous microbial culture isolated from hydrocarbon unpolluted soil are presented. Mixture of biodiesel/diesel B10 (10% biodiesel/diesel (v/v) and biodiesel B100 (FAME, fatty acid methyl ester) was tested. The results were compared to the results of reference fuel (euro diesel). From diesel and biodiesel fuel, mixed microbial cultures were isolated as well. The results showed that the mixed microbial population that has not previously been exposed to petroleum hydrocarbons in the laboratory degraded FAME completely after six days and pure diesel fuel but to a lower extent. The difference was observed in the growth rate depending on the source of carbon (FAME or pure eurodiesel) as well as in growth conditions, depending on the culture medium composition. From the study results, it can be concluded that in the entire fuel production, storage and supply chain after FAME addition there will be the enhanced microbial proliferation, which can, if "good housekeeping practice" will not be implemented, resulted with fuel quality reduction and biocorrosion.
The aim was to meet company authorities what kind of problems the company will face after the introduction of biodiesel (fatty acid methyl ester FAME) in the production process and to introduce the new fast and precise methods for fuel microbial infection detection. In addition, based on laboratory research result, company’s fuel and distribution supply chain conditions and experience from petroleum industry in the world preventive and protective measures were proposed.
fuels, biofuels, quality, biodegradatio
