Učinak kortikalnog autopresatka, komercijalnog pripravka demineralizirane koštane matrice, demineralizirane koštane matrice telećeg ploda i praška od epifizealnog diska telećeg ploda na cijeljenje kostiju u kunića.

Abstract

Trauma, developmental anomalies, infection, and pathological injuries can lead to defects in the bony skeleton and it is a current challenge for surgeons and investigators to restore lost tissues. This study was designed to evaluate the effects of cortical autograft, commercial-demineralized bone matrix (DBM), calf fetal demineralized bone matrix and calf fetal growth plate powder on bone healing in a rabbit model. Five round defects were created on a tibial bone with an electric drill in ten adult white New Zealand rabbits. One of the defects was left empty as a control group but the other defects were filled with different biomaterials. Radiographs of each hind limb were taken postoperatively on the 1st day and at the 2nd, 4th and 6th weeks post injury, to evaluate the bone healing criteria of the defect. The operated tibial bones were removed on the 42nd postoperative day and evaluated for histopathology criteria. Based on the radiology and histopathology findings of the present study, autografts, commercial-demineralized bone matrix, calf fetal demineralized bone matrix and calf fetal growth plate groups demonstrated superior osteogenic potential in healing of the tibial bone defects in the rabbit model. However, the control group was inferior to the autograft, commercial-DBM, calf fetal DBM and calf fetal growth plate powder groups at this stage.Trauma, razvoj anomalija, infekcije i patološka stanja mogu dovesti do različitih defekata kostiju. Njihovo cijeljenje i obnova izgubljenih tkiva predstavlja veliki izazov za kirurge i istraživače. Ovo istraživanje poduzeto je sa svrhom da se prosude učinci kortikalnog autopresatka, komercijalne demineralizirane koštane matrice, demineralizirane koštane matrice telećeg ploda i praška od epifizealnog diska telećeg ploda na cijeljenje kostiju na kunićjem modelu. Pet okruglih defekata učinjeno je električnim svrdlom na goljeničnoj kosti u 10 bijelih novozelandskih kunića. Kontrolni defekt ostavljen je prazan dok su ostali bili ispunjeni različitim biomaterijalima. Za procjenu cijeljenja defekta učinjene su radiografske slike stražnjih nogu prvog, drugog, četvrtog i šestog tjedna nakon ranjavanja. Operirane goljenične kosti uzete su 42. dana nakon operacije te pretražene patohistološki. Radiološki i patohistološki nalazi pokazali su da autopresadak, komercijalni pripravak demineralizirane koštane matrice, demineralizirana koštana matrica telećeg ploda i prašak od epifizealnog diska telećeg ploda imaju dobar učinak na cijeljenje defekta goljeničnog zgloba u pokusnih kunića. Cijeljenje defekta u kunića kontrolne skupine bilo je slabije